| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,192,754 | C→A | 9.4% | intergenic (+135/‑67) | PP_4572 → / → PP_4573 | hypothetical protein/AAA family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,192,754 | 0 | C | A | 9.4% | 136.6 / 2.8 | 57 | intergenic (+135/‑67) | PP_4572/PP_4573 | hypothetical protein/AAA family ATPase |
| Reads supporting (aligned to +/- strand): ref base C (30/21); new base A (3/3); total (33/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01 | |||||||||||
CTGAGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGC > NC_002947/5192616‑5192901 | ctgaGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCtta‑tta < 7:160067/149‑1 (MQ=255) aCAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGGCCAATTACCGGGGTAAAACCCCCCCAACAAACTTA‑TTTGgc > 2:311092/1‑149 (MQ=255) aGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGATACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTAAAACCTTA‑TTAGGCGCgg > 5:137247/1‑149 (MQ=255) gCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCggg < 3:247764/149‑1 (MQ=255) aCGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCGCCTAATAAGGTT‑GTAGGGGCGGGTTTAc < 1:313327/149‑1 (MQ=25) gggCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCcg > 8:251713/1‑149 (MQ=255) ggCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCcgc < 1:16529/149‑1 (MQ=255) gCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCGCCTAATAAGGTT‑GTAGGGGCGGGTTTACCcgcg < 2:18519/149‑1 (MQ=25) aTTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCgg > 6:2935/1‑136 (MQ=255) aTTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCgg < 5:2935/136‑1 (MQ=255) cAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTTAAACCCCCCCGACAACCGTA‑TTATGCGCGGGGGTACAcgcgcacac > 5:35490/1‑144 (MQ=255) gACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTTGGCGCGGGTTTTCCCGGGAGGAGGccc > 7:262694/1‑149 (MQ=255) aCTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCg > 4:204988/1‑149 (MQ=255) aaTACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGGCCCGACACCCTTT‑TTAGGCGCGGGTTTTCCCGCGCAGCGGCCCggc > 7:268257/1‑145 (MQ=255) cccGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTGTACCCGCGAAGAGGCCCGCAAAGccc > 5:275033/1‑149 (MQ=255) cccGGGCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGTAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACACCCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGccc < 3:217640/149‑1 (MQ=255) cGGNATGCGTTTCCAGCCGCCAGGCCCCGCGTTATAGTCGTAAACAAATCCAGATCATGTTCTGCCTTTTC‑GGGCCTCTT‑CGCGGTTAAACCCCCCCCTACAACCTTA‑TTCGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTa < 6:315763/149‑1 (MQ=38) gTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTTAACCCGCCCCTTCAACCGTA‑TTTGGCGCGGGGTTACCCGCGAAGCGGCCCGCAAAGCCCGaaa > 5:306685/1‑149 (MQ=255) cAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACaaa > 2:246582/1‑149 (MQ=255) ccgccAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAccc < 7:251713/149‑1 (MQ=255) cgccAGGCCCAGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGGTTAAACGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAcccc > 3:169833/1‑149 (MQ=255) gccAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAcccc > 4:146735/1‑148 (MQ=255) gCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGaa < 4:117854/149‑1 (MQ=255) ccccGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGCTAAACCCGCCCCTACCACAATA‑TAAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCACCAGGaac > 8:288464/1‑148 (MQ=38) ccGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCTTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAc > 5:78593/1‑137 (MQ=255) ccGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCTTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAc < 6:78593/137‑1 (MQ=255) cgcgTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAAACCGCCCCGACAACCTTA‑TTATGCGCCGGGTTTCCCCCGAAGAGGCCCGCAAACCCCTAAACAGAGGACAAACCCCCATGAAGTTc > 5:40688/1‑149 (MQ=255) tCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGaa < 4:89320/142‑1 (MQ=255) tCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGaa > 3:89320/1‑142 (MQ=255) gAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCcgc > 2:40027/1‑148 (MQ=255) aCAAATCACTATCATGTGCTCCCTTTTC‑GGGCCTCTT‑CG‑GGGTTAACCCGCCCCTAAAACCATATTTCGGCGCGGTTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACt < 6:35490/149‑1 (MQ=255) aTCCATATAATTTGCTGCCTTTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGt < 5:14564/127‑1 (MQ=255) tCCAGATCATATGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTTGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTc > 8:261290/1‑149 (MQ=255) aGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTTAAACCGCCCCTACAACCGTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAAAGGACAAACCCCCAAGAAGTTAGAAGGCACCCGCCACTACGTCGc > 4:162107/1‑148 (MQ=38) aGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAg > 7:265715/1‑129 (MQ=255) aGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACATTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAg < 8:265715/129‑1 (MQ=255) gcCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCGACACCCTTA‑TTAGGCGCGGGTGTACCCGCGAAGAGGCCCGCAACGCCCGAAACAGAGGCCCACCCCCCAGGCAGGTCGAAGGCCCCCGCGACGACGGCGCCACAGAGGAc > 2:277402/1‑146 (MQ=25) ccTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCAGCCGCGACTACGTCGCCACAGATGACCTg > 5:288975/1‑148 (MQ=255) ccTGTTC‑GGGCCTCTT‑CGCGGGTAAAACCGCCCCTAAAACCATA‑TTAGGCGCGGGTTTTACCGCGAAGAGGCCCGCAAAACCCGAAAAAGAGGGCAAACCCCCATGAAGTTCGAAGGCACCCGCGACAAAGTCGCCACACATGCCCTg > 4:122105/1‑148 (MQ=37) tGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGaaa > 8:52042/1‑149 (MQ=255) tgc‑GGGCCTCTT‑CGCGGGTAAACCCGCGCCTAATAAGGTT‑GTAGGGGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGACACt > 8:210513/3‑149 (MQ=21) gggCCTCTT‑CGCGGGTAAACCCTCCCCTACAACCTTA‑TTCGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTgg < 4:148659/148‑1 (MQ=255) gggCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTTGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTgg < 7:261290/148‑1 (MQ=255) ctctT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGTCAAACCCCCATGAAGTTCGAAGGCACCCGCGACTAAGTCGCCACAGATGCCCTGAAACTGGCGGt > 5:199091/1‑148 (MQ=255) ctctT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGt > 5:80898/1‑148 (MQ=255) cgcgGGTAAACCGGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTaaa < 3:122105/146‑1 (MQ=255) cgcgGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAAc < 6:137247/147‑1 (MQ=255) cgGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCgg > 7:192554/1‑149 (MQ=255) gTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCCCCCGCGACTACGACGCCACAGCTGACCTGAAACTGGCGGTAAACGCGGCCa > 6:278534/1‑149 (MQ=255) tAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGTACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCAt < 1:246582/149‑1 (MQ=255) ctccGCCCCTACAACCTTA‑TTCGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTAc < 1:277402/147‑1 (MQ=255) aCCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAATCAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGcc < 7:95135/144‑1 (MQ=255) aCCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAGCAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGcc > 8:95135/1‑144 (MQ=255) ccGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTAccc < 2:22319/149‑1 (MQ=255) cGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCt < 8:262694/149‑1 (MQ=255) aCAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGc > 6:160110/1‑149 (MQ=255) aCAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGc > 3:97227/1‑149 (MQ=255) | CTGAGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTC‑GGGCCTCTT‑CGCGGGTAAACCCGCCCCTACAACCTTA‑TTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGC > NC_002947/5192616‑5192901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |