Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,202,981	C→A	5.6%	L430M (CTG→ATG) ‡	PP_4583 →	putative Peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,202,981	0	C	A	5.6%	234.3 / 3.4	72	L430M (CTG→ATG) ‡	PP_4583	putative Peptidase
Reads supporting (aligned to +/- strand):  ref base C (37/31);  new base A (2/2);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

AAGCGCTCAACCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCCTGCTGGAGCGCGGCGTGGCCTTT  >  NC_002947/5202835‑5203127                                                                                                                                                   |                                                                                                                                                    aaGCGCTCAACCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTg                                                                                                                                                   >  2:204024/1‑149 (MQ=255)    cgcTCAACCGCCCGGCCCAGGTGCACCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTg                                                                                                                                                >  6:318334/1‑149 (MQ=255)         aaCCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGAt                                                                                                                                          >  8:282417/1‑149 (MQ=255)         aaCCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCAGGGTG‑CCgtc                                                                                                                                          <  4:99013/149‑3 (MQ=255)         aaCCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCAGGGTG‑CCgtc                                                                                                                                          >  3:99013/1‑147 (MQ=255)          aCCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGAt                                                                                                                                          >  3:148289/1‑148 (MQ=255)           ccGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGAt                                                                                                                                          >  2:92324/1‑147 (MQ=255)           ccGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCa                                                                                                                                        <  1:204024/149‑1 (MQ=255)            cGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCAGGGTG‑CCgtc                                                                                                                                          >  3:132784/1‑144 (MQ=255)            cGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCAGGGTG‑CCgtc                                                                                                                                          <  4:132784/146‑3 (MQ=255)             gCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGc                                                                                                                                      >  7:197886/1‑149 (MQ=255)             gCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGc                                                                                                                                      <  6:110366/149‑1 (MQ=255)              cccGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑c                                                                                                                                              <  7:34583/140‑1 (MQ=255)              cccGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑c                                                                                                                                              >  8:34583/1‑140 (MQ=255)                cGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTg                                                                                                                                   <  4:148289/149‑1 (MQ=255)                cGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTg                                                                                                                                   <  8:197886/149‑1 (MQ=255)                      aGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTAcgc                                                                                                                             >  8:227611/1‑149 (MQ=255)                        gTGCGCCAACTGACGTTGCCCACGGGCGCCAAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTAcgccg                                                                                                                           >  4:280729/1‑149 (MQ=255)                             ccAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACgccgccgcc                                                                                                                      <  4:217474/149‑1 (MQ=255)                              cAACTGACGTTGCCCACGGGCGCCCAGGTGTTGCTCAAGCAATCCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCa                                                                                                                     <  7:227611/149‑1 (MQ=255)                               aaCTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAg                                                                                                                    >  2:162016/1‑149 (MQ=255)                                  tGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCa                                                                                                                     <  6:288258/145‑1 (MQ=255)                                  tGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCa                                                                                                                     >  5:288258/1‑145 (MQ=255)                                       ttGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTggg                                                                                                            <  1:144349/149‑1 (MQ=255)                                            cccGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGa                                                                                                       <  1:79412/147‑1 (MQ=255)                                            cACGGGCGCCCAGGTGGTGATCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGa                                                                                                       >  5:4166/1‑149 (MQ=255)                                               gggCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCg                                                                                                    >  2:137684/1‑149 (MQ=255)                                                   gcCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGc                                                                                                 >  3:190893/1‑148 (MQ=255)                                                    cccAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCa                                                                                               <  6:43928/149‑1 (MQ=255)                                                          tggtgCTCAAGCAAACCCCGGTGCCCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCActgtac                                                                                         <  7:282417/149‑1 (MQ=255)                                                               cTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCActgtacctgt                                                                                     <  3:280729/148‑1 (MQ=255)                                                                  aaGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTGTACGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCActgtacctgtac                                                                                   <  1:92324/148‑1 (MQ=255)                                                                  aaGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACgg                                                                                 <  1:162016/149‑1 (MQ=255)                                                                    gCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGc                                                                                                           <  8:191739/121‑1 (MQ=255)                                                                    gCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGc                                                                                                           >  7:191739/1‑121 (MQ=255)                                                                        aCCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATgg                                                                           >  6:318196/1‑149 (MQ=255)                                                                            cGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGcc                                                                       >  6:150342/1‑149 (MQ=255)                                                                               tGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTAt                                                                    <  4:190893/149‑1 (MQ=255)                                                                                 cTCGGTGCATTCGATGCCGATGACTATGTCAGAGAGCGCCTGTGGCCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATgg                                                                  >  7:99351/1‑149 (MQ=255)                                                                                          ttCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCt                                                         >  6:192627/1‑149 (MQ=255)                                                                                          ttCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCt                                                         <  1:137684/149‑1 (MQ=255)                                                                                             gATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCg                                                       <  5:190100/148‑1 (MQ=255)                                                                                              aTGCCGATGACTATGTCATTGAGCTCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGTCTATGGTGCCTATGGCGAAAGCCTCGAc                                                     <  1:13039/149‑1 (MQ=255)                                                                                                  cGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCt                                                                                                               >  5:7196/1‑87 (MQ=255)                                                                                                  cGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCt                                                                                                               <  6:7196/87‑1 (MQ=255)                                                                                                  cGATGACTATGTCAGTGAGCGACTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCt                                                 >  2:72226/1‑149 (MQ=255)                                                                                                   gATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTg                                                >  7:67927/1‑149 (MQ=255)                                                                                                    aTGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTgg                                               >  6:8551/1‑149 (MQ=255)                                                                                                        cTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTtct                                           <  8:67927/149‑1 (MQ=255)                                                                                                        cTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACACTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTtct                                           >  5:312729/1‑149 (MQ=255)                                                                                                          aTGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTca                                         >  4:69473/1‑149 (MQ=255)                                                                                                            gTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGAc                                                     >  1:73037/1‑135 (MQ=255)                                                                                                            gTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGAc                                                     <  2:73037/135‑1 (MQ=255)                                                                                                             tCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTcaca                                       <  5:318196/148‑1 (MQ=255)                                                                                                                     cgcCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCt                              <  6:78408/149‑1 (MQ=255)                                                                                                                      gcCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCt                              >  3:238646/1‑148 (MQ=255)                                                                                                                      gcCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTg                             <  4:238646/149‑1 (MQ=255)                                                                                                                        cTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACAt                                      >  5:33521/1‑138 (MQ=255)                                                                                                                        cTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACAt                                      <  6:33521/138‑1 (MQ=255)                                                                                                                        cTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAg                           >  1:273283/1‑149 (MQ=255)                                                                                                                         tgtgGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGc                          >  1:17221/1‑149 (MQ=255)                                                                                                                           tgGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCct                        >  2:227938/1‑149 (MQ=255)                                                                                                                               cAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGa                            >  1:12984/1‑141 (MQ=255)                                                                                                                               cAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGa                            <  2:12984/141‑1 (MQ=255)                                                                                                                               cAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCctgctg                    <  6:312729/149‑1 (MQ=255)                                                                                                                                aaCCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCctgctg                    >  5:97219/1‑148 (MQ=255)                                                                                                                                aaCCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCctgctg                    >  3:177902/1‑148 (MQ=255)                                                                                                                                aaCCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCGGGTTCTCACCTGCGCGCCTGAGCCTGCTgg                   >  5:92677/1‑149 (MQ=255)                                                                                                                                   cGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACgg                                                                                 <  3:258274/84‑1 (MQ=255)                                                                                                                                   cGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACgg                                                                                 >  4:258274/1‑84 (MQ=255)                                                                                                                                   cGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCCTGCTGGAgc                <  6:4166/149‑1 (MQ=255)                                                                                                                                               cACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCCTGCTGGAGCGCGGCGTGGCCt    >  3:267369/1‑149 (MQ=255)                                                                                                                                                 cccTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCCTGCTGGAGCGCGGCGTGGCCttt  <  4:177902/149‑1 (MQ=255)                                                                                                                                                  ccTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTtctc                                          >  6:207872/1‑108 (MQ=255)                                                                                                                                                  ccTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTtctc                                          <  5:207872/108‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AAGCGCTCAACCGCCCGGCCCAGGTGCGCCAACTGACGTTGCCCACGGGCGCCCAGGTGGTGCTCAAGCAAACCCCGGTGCTCGGTGCATTCGATGCCGATGACTATGTCAGTGAGCGCCTGTGGGCAACCGCAGCGGACGGCACCCTGGTG‑CCGATCAGCCTGGTACGCCGCCGCCAGGACCTGGGCAAGACCGTGCCACTGTACCTGTACGGCTATGGTGCCTATGGCGAAAGCCTCGACCCCTGGTTCTCACATGCGCGCCTGAGCCTGCTGGAGCGCGGCGTGGCCTTT  >  NC_002947/5202835‑5203127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑