Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,210,162 A→C 7.9% intergenic (‑28/+150) rnd ← / ← PP_4592 ribonuclease D/metal dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,210,1620AC 7.9% 133.9 / 2.8 49intergenic (‑28/+150)rnd/PP_4592ribonuclease D/metal dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (21/24);  new base C (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

GTAAAAGGTGTCTACCCGCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGTTCGCGGGCGTGCCCGCTC  >  NC_002947/5210015‑5210301
                                                                                                                                                   |                                                                                                                                           
gTAAAAGGTGTCTACCCGCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAg                                                                                                                                            >  3:222327/1‑149 (MQ=255)
   aaaGGTGTCTACCCGCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGta                                                                                                                                          >  5:200950/1‑148 (MQ=255)
          tCTACCCGCAAGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTTACATTGGCGCGCAGTATATACGgc                                                                                                                                  >  7:136233/1‑149 (MQ=255)
            tACCCGCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGgcgc                                                                                                                                >  3:221115/1‑149 (MQ=255)
              cccGCATGAATTCGGTGTCGACGGCGACGAAGGGCATTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCCGTATATACTGcgcgc                                                                                                                              <  7:182376/149‑2 (MQ=255)
                 gCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGgcg                                                                                                                                 >  1:141899/1‑143 (MQ=255)
                 gCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGgcg                                                                                                                                 <  2:141899/143‑1 (MQ=255)
                   aTGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACt                                                                                                                         <  1:163948/149‑1 (MQ=255)
                       aTTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCgg                                                                                                                     <  8:10736/149‑1 (MQ=255)
                       aTTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCgg                                                                                                                     <  3:76099/149‑1 (MQ=255)
                       aTTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGGCGCGGCAGTTTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCg                                                                                                                      <  5:175582/148‑1 (MQ=255)
                               tCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAAc                                                                                                              <  8:82143/148‑1 (MQ=255)
                                 gACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCCGTATATACTGcgcgccaatg                                                                                                                         >  6:232452/1‑129 (MQ=255)
                                 gACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCCGTATATACTGcgcgccaatg                                                                                                                         <  5:232452/135‑7 (MQ=255)
                                  tcGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTc                                                                                                          <  6:200950/148‑1 (MQ=255)
                                  aCGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTc                                                                                                          >  8:179607/1‑149 (MQ=255)
                                    ggCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTccc                                                                                                        >  3:108576/1‑149 (MQ=255)
                                           aaGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTc                                                                                                 <  4:222327/149‑1 (MQ=255)
                                            aGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCACGCTTTTCg                                                                                                >  4:100814/1‑149 (MQ=255)
                                                cAGTTGGTGCCAGTCTCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCggggg                                                                                            <  8:146768/149‑1 (MQ=255)
                                                        gCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGtatat                                                                                                                                       >  7:227224/1‑98 (MQ=255)
                                                        gCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGtatat                                                                                                                                       <  8:227224/98‑1 (MQ=255)
                                                         ccAGTCGCGGCAGTGTTCGTCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCAcc                                                                                   <  5:328470/149‑1 (MQ=255)
                                                         ccAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCAcc                                                                                   <  4:221115/149‑1 (MQ=255)
                                                           aGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCt                                                                                 >  3:195085/1‑149 (MQ=255)
                                                               gggggaGTGTTCGGCCAGGCTCTGGTGGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCACACTTACATTGGCGCGCAGTTTATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGTGCAGTCCACCCtggg                                                                              <  8:136233/143‑3 (MQ=255)
                                                                     gtgtTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCgg                                                                                               <  3:62226/125‑1 (MQ=255)
                                                                     gtgtTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCgg                                                                                               >  4:62226/1‑125 (MQ=255)
                                                                        ttCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGgcg                                                                    <  6:6874/149‑1 (MQ=255)
                                                                        ttCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGgc                                                                     <  1:140803/148‑1 (MQ=255)
                                                                        ttCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGgc                                                                     <  7:179607/148‑1 (MQ=255)
                                                                             ccAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCACGccc                                                               >  8:302392/1‑149 (MQ=255)
                                                                               aGGCTCTGGTCGTCACGGATCCTGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCcgc                                                             <  2:260121/149‑1 (MQ=255)
                                                                                 gCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCcgctc                                                           >  8:98261/1‑147 (MQ=255)
                                                                                  ctctGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCcgctcc                                                          <  7:98261/149‑4 (MQ=255)
                                                                                      ggTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGAGGGCGCGCCCGCGAATg                                                        >  6:224528/1‑147 (MQ=255)
                                                                                            cACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCaa                                                 >  8:176449/1‑148 (MQ=255)
                                                                                                    cAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGgtgt                                         <  4:195085/148‑1 (MQ=255)
                                                                                                     aGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTAt                                       <  3:100814/149‑1 (MQ=255)
                                                                                                                      acaAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCgg                      >  7:180292/1‑149 (MQ=255)
                                                                                                                              ctcCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCGGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGTTCGCgg              <  1:155828/149‑1 (MQ=255)
                                                                                                                                cccACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGgtgt                                         >  1:58991/1‑120 (MQ=255)
                                                                                                                                cccACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGgtgt                                         <  2:58991/120‑1 (MQ=255)
                                                                                                                                 ccACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGTTCGCGGGCg           >  4:90984/1‑149 (MQ=255)
                                                                                                                                     tAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACCGTGTATGGCCCGGGTTTGCCCGGGTTCGCGGGCGTGcc       >  7:184813/1‑149 (MQ=255)
                                                                                                                                         aTTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGt                    <  1:247178/132‑1 (MQ=255)
                                                                                                                                         aTTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGt                    >  2:247178/1‑132 (MQ=255)
                                                                                                                                          ttGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCAGTGGGAGCCGGCGCGCCCGCGAAAGAAGGCAACACCGTGTATGGCCCCGGTTTGCCCGGGTTAGCCGCCCTGCCCGcac  >  6:40873/1‑147 (MQ=255)
                                                                                                                                          tgcccgcgcCGTATATACTGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGTTCGCGGGCGTGCCCGCTc  >  2:229339/5‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GTAAAAGGTGTCTACCCGCATGAATTCGGTGTCGACGGCGACGAAGGGCAGTTGGTGCCAGTCGCGGCAGTGTTCGGCCAGGCTCTGGTCGTCACGGATCCAGTGAATTTCGATGGCCACAAGGCTCTCCCACTAACATTGGCGCGCAGTATATACGGCGCGGGCACTGCGGGTGAAACTGTCCCGCTTTTCGGGGGCAGTCCACCCTGTGGGAGCGGGCGCGCCCGCGAATGAAGGCAACACGGTGTATGGCCCGGGTTTGCCCGGGTTCGCGGGCGTGCCCGCTC  >  NC_002947/5210015‑5210301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑