Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,212,472	C→T	13.3%	intergenic (+36/+58)	PP_4593 → / ← PP_4594	hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,212,472	0	C	T	13.3%	111.3 / 8.4	45	intergenic (+36/+58)	PP_4593/PP_4594	hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base T (3/3);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

GGTAGCCGCCGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAG  >  NC_002947/5212325‑5212616                                                                                                                                                    |                                                                                                                                                 gttAGCCGCCTAAGGGAACGGCACCCACGACGAAGCCCTCTACAGCCTTCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGc                                                                                                                                                  <  2:291721/146‑1 (MQ=255)     gccgccGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGcc                                                                                                                                             >  7:164075/1‑149 (MQ=255)         ccgAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCt                                                                                                                                         <  5:85314/149‑1 (MQ=255)                    ggCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtc                                                                                                                              <  1:12542/149‑1 (MQ=255)                          aaCGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                          >  2:214471/1‑147 (MQ=255)                          aaCGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                          >  6:126087/1‑147 (MQ=255)                            cgacgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACTGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGc                                                                                                                      <  4:37608/149‑1 (MQ=38)                              acgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGCACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCgg                                                                                                                    <  2:197979/149‑1 (MQ=255)                              acgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCgg                                                                                                                    <  5:126087/149‑1 (MQ=255)                                 gcagcGTCAACCGCCTGCGCAAGGAAAGGTTGCTGCTCTAGGACAAGATCGAACGTATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGtt                                                                                                                 <  6:199214/145‑1 (MQ=255)                                     cGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAc                                                                                                              >  1:274086/1‑148 (MQ=255)                                              ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                    >  6:35969/1‑149 (MQ=255)                                                 gcgcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGcc                                                                                                 >  5:239517/1‑149 (MQ=255)                                                  cgcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCt                                                                                                <  2:274086/149‑1 (MQ=255)                                                        gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGcc                                                                                          <  6:239517/149‑1 (MQ=255)                                                             gTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcac                                                                                     <  3:175857/149‑1 (MQ=255)                                                             gTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTca                                                                                      >  3:213490/1‑148 (MQ=255)                                                              ttGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcaca                                                                                    >  1:243894/1‑149 (MQ=255)                                                                 cTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcacaca                                                                                  <  2:41348/148‑1 (MQ=255)                                                                 cTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcaca                                                                                    >  1:41348/1‑146 (MQ=255)                                                                  tGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGa                                                                                                        >  7:300537/1‑125 (MQ=255)                                                                  tGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGa                                                                                                        <  8:300537/125‑1 (MQ=255)                                                                          gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAgcg                                                                        >  5:28613/1‑149 (MQ=255)                                                                            cAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCt                                                                      >  3:208627/1‑149 (MQ=255)                                                                                 tCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCaa                                                                 >  4:309889/1‑149 (MQ=255)                                                                                    aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGc                                                              <  8:164075/149‑1 (MQ=255)                                                                                        gATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCa                                                          >  2:41439/1‑149 (MQ=255)                                                                                                         aaTCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAg                                         >  8:28242/1‑149 (MQ=255)                                                                                                            ccTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                            <  4:125300/143‑1 (MQ=255)                                                                                                            ccTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                            >  3:125300/1‑143 (MQ=255)                                                                                                                aCAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                  <  1:41439/149‑1 (MQ=255)                                                                                                                 cAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                  <  4:213490/148‑1 (MQ=255)                                                                                                                           ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                  <  3:259871/138‑1 (MQ=255)                                                                                                                           ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                  >  4:259871/1‑138 (MQ=255)                                                                                                                           ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGa                        <  5:35969/148‑1 (MQ=255)                                                                                                                            tGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                             <  1:301320/142‑1 (MQ=255)                                                                                                                            tGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGAt                       <  4:208627/148‑1 (MQ=255)                                                                                                                              aaTCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATccc                    <  7:28242/149‑1 (MQ=255)                                                                                                                                       gCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACaa           >  5:282204/1‑149 (MQ=255)                                                                                                                                            ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCg        >  2:80830/1‑147 (MQ=255)                                                                                                                                               gcgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcag     >  3:187294/1‑147 (MQ=255)                                                                                                                                                cgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag  >  2:217444/1‑149 (MQ=37)                                                                                                                                                cgGGTAAACCCGCTCCTACGACGGCGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                           <  4:111148/124‑1 (MQ=37)                                                                                                                                                cgGGTAAACCCGCTCCTACGACGGCGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                           >  3:111148/1‑124 (MQ=37)                                                                                                                                                 gggTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAg                                         <  2:276797/109‑1 (MQ=21)                                                                                                                                                 gggTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAg                                         >  1:276797/1‑109 (MQ=21)                                                                                                                                                    |                                                                                                                                                 GGTAGCCGCCGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAG  >  NC_002947/5212325‑5212616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑