Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,245,902	C→T	7.0%	R920Q (CGG→CAG)	PP_4623 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,245,902	0	C	T	7.0%	166.3 / 2.6	57	R920Q (CGG→CAG)	PP_4623	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/22);  new base T (2/2);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

GTACGATCCCCCACTCCACCACCACGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCC  >  NC_002947/5245756‑5246049                                                                                                                                                   |                                                                                                                                                     gTACGATCCCCCACTCCACCACCACGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCg                                                                                                                                                     >  5:118071/1‑148 (MQ=255)     gaccccccACTCCACCACCACGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGc                                                                                                                                                >  2:31129/4‑149 (MQ=255)                  accaccacGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTc                                                                                                                                       <  4:254573/145‑1 (MQ=255)                  accaccacGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTc                                                                                                                                       >  3:254573/1‑145 (MQ=255)                     accacGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGAc                                                                                                                                <  6:118071/149‑1 (MQ=255)                          ggACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATACCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACCCCCCTGCCTCGGGCGACGG‑CAt                                                                                                                          >  5:32117/1‑149 (MQ=255)                              gAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCTGCACGCCCCTGCCTCGGGCGACGG‑CATCCAg                                                                                                                      >  8:101282/1‑149 (MQ=255)                              gAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAg                                                                                                                      >  6:119821/1‑149 (MQ=255)                                     gCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCaccacc                                                                                                               >  5:134991/1‑149 (MQ=255)                                              cGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCCCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCa                                                                                                      <  8:14901/149‑1 (MQ=255)                                                 cGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCg                                                                                                   >  2:132018/1‑149 (MQ=255)                                                    cgcTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCg                                                                                                <  8:186078/149‑1 (MQ=255)                                                           ttCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGcac                                                                                                                   <  2:323505/123‑1 (MQ=255)                                                           ttCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGcac                                                                                                                   >  1:323505/1‑123 (MQ=255)                                                             cAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCa                                                                                         <  4:260369/147‑1 (MQ=255)                                                                cGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CGTCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGc                                                                                    >  6:280513/1‑149 (MQ=255)                                                                       gTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCg                                                                              <  5:119821/148‑1 (MQ=255)                                                                          gATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATc                                                                           >  3:160810/1‑148 (MQ=255)                                                                          gATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATc                                                                           >  4:298209/1‑148 (MQ=255)                                                                           atcatcCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCaa                                                                         <  7:80838/149‑1 (MQ=255)                                                                             catcCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCACGGGCGACCG‑CATCCAGCACCACCCCAATGCCAACGCCGGCGCCCAGTTGCTCTAAGATAAATg                                                                       >  6:219908/1‑149 (MQ=255)                                                                              atcCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGc                                                                      >  8:64120/1‑149 (MQ=255)                                                                                   ccTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTc                                                                  >  5:209546/1‑148 (MQ=255)                                                                                       aTACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAg                                                               <  1:132018/147‑1 (MQ=255)                                                                                           gTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGAc                                                         >  1:316177/1‑149 (MQ=255)                                                                                            tttCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAAt                                                                        <  6:241523/133‑1 (MQ=255)                                                                                            tttCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAAt                                                                        >  5:241523/1‑133 (MQ=255)                                                                                               ctcCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGc                                                     >  4:194816/1‑149 (MQ=255)                                                                                                      tCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATaa                                              <  3:298209/149‑1 (MQ=255)                                                                                                      tCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATaa                                              >  5:95503/1‑149 (MQ=255)                                                                                                           gACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCa                                         >  7:211859/1‑149 (MQ=255)                                                                                                            aCAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCaa                                                                >  4:212962/1‑125 (MQ=255)                                                                                                            aCAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCaa                                                                <  3:212962/125‑1 (MQ=255)                                                                                                             cAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGt                                                                    <  3:150027/120‑1 (MQ=255)                                                                                                               ggATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCAcgcg                                     <  8:211859/149‑1 (MQ=255)                                                                                                                  tCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCCTCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTc                                  >  5:218399/1‑149 (MQ=255)                                                                                                                  tCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTc                                  >  7:298861/1‑149 (MQ=255)                                                                                                                   ccccGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCt                                 >  5:40494/1‑149 (MQ=255)                                                                                                                     ccGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGAACGCGGTCTTg                               >  3:151073/1‑149 (MQ=255)                                                                                                                     ccGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACACCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTg                               <  5:219908/149‑1 (MQ=255)                                                                                                                      cGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTgg                              <  3:194816/149‑1 (MQ=255)                                                                                                                       gATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGc                             <  7:64120/149‑1 (MQ=255)                                                                                                                           cGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATa                         <  6:209546/149‑1 (MQ=255)                                                                                                                            gCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGGACTTCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCGCGATAACGGCACGCGGTCTTGGCAAt                          >  1:76373/1‑148 (MQ=255)                                                                                                                                ggTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTg                    <  1:31129/149‑1 (MQ=255)                                                                                                                                 gTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGc                   <  4:7060/149‑1 (MQ=255)                                                                                                                                 gTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGc                   >  3:7060/1‑149 (MQ=255)                                                                                                                                     ccccTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAg               >  7:205057/1‑149 (MQ=255)                                                                                                                                     ccccTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCCGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAg               >  3:156352/1‑149 (MQ=255)                                                                                                                                      cccTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGt              >  2:309150/1‑149 (MQ=255)                                                                                                                                        cTATCGGCGGCTGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTc             >  4:257668/1‑148 (MQ=255)                                                                                                                                        cTATCGGCGGCTGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTc             <  3:257668/148‑1 (MQ=255)                                                                                                                                        cTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCg            >  4:107015/1‑149 (MQ=255)                                                                                                                                         tATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATaa                                              >  1:153645/1‑114 (MQ=255)                                                                                                                                         tATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATaa                                              <  2:153645/114‑1 (MQ=255)                                                                                                                                           tCGGCGGCTGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTg          <  7:101282/148‑1 (MQ=255)                                                                                                                                            cggcggCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGc         <  6:40494/148‑1 (MQ=255)                                                                                                                                                  ccGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGcc  <  6:95503/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTACGATCCCCCACTCCACCACCACGGACGAAATACGCTCGGCAGCGTCGGCGCTGTCTTCAGCGGCTTCGTTGATCATCCGCCTGATACGTTTCTCCAGGTCCTGGACAGGATCCCCGATACGCCCGGTCTCCCCTATCGGCGGCCGCACGCCCCTGCCTCGGGCGACGG‑CATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCC  >  NC_002947/5245756‑5246049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑