| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,257,883 | T→C | 5.8% | intergenic (‑82/+62) | PP_4634 ← / ← PP_4635 | alpha/beta family hydrolase/trans‑2‑enoyl‑CoA reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,257,883 | 0 | T | C | 5.8% | 264.0 / 4.5 | 87 | intergenic (‑82/+62) | PP_4634/PP_4635 | alpha/beta family hydrolase/trans‑2‑enoyl‑CoA reductase |
| Reads supporting (aligned to +/- strand): ref base T (34/47); new base C (3/2); total (38/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
GCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAG > NC_002947/5257736‑5258031 | gccgAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTa > 4:53465/1‑149 (MQ=255) gCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAggg < 1:113004/148‑1 (MQ=255) tGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAgggg > 6:65860/1‑147 (MQ=255) tATCCGTCGGCCGCGGAGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAggggg > 2:5646/1‑146 (MQ=255) tATCCGTCGGCCGCGGAGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAggggg < 1:5646/146‑1 (MQ=255) tCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAAc < 3:53465/149‑1 (MQ=255) gTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCtg < 1:248041/149‑1 (MQ=255) ggCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGGGAACCtgtg < 1:258970/148‑1 (MQ=255) ggCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCtgtg < 5:159537/148‑1 (MQ=255) ggCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTgg < 7:57018/149‑1 (MQ=255) ccGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGa < 1:267976/149‑1 (MQ=255) ccGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCGGTgg > 8:248588/1‑147 (MQ=255) ccGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCACTCCTACAGGGGGAGAACCTGTgg > 5:215703/1‑147 (MQ=255) agcgGGGTTGCGAAATGGGGTAGCTGTTTTGATGATGCTACTAATGGCTCATGTCATGTTGTTGTGCGCAAAAACTAGAAAAGATTTTGTAGCCTTTACTGGCCTCTTCGCGGGTGAACCCTCTCCTACAGGGGGAGAACCTGTGGGa < 8:267853/147‑1 (MQ=37) ggggggGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCgg < 5:50050/149‑1 (MQ=255) ggggTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCgg < 6:48974/149‑1 (MQ=255) ggggTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCgg < 2:319478/149‑1 (MQ=255) gCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCgggttcac < 6:319524/149‑6 (MQ=255) gCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCATGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGc > 1:232616/1‑149 (MQ=255) gAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGccc > 8:179829/1‑149 (MQ=255) gggTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAag < 8:179770/149‑1 (MQ=255) gggTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCACTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAag < 6:215703/149‑1 (MQ=255) ggTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAaga < 4:97095/149‑1 (MQ=255) gTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAagag < 5:314512/149‑1 (MQ=255) gTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGCAAGCCCGCTCCCACAGGTTCTCCCCCTGTAGGAGCGGGTTCACCCGCGAagag < 6:241977/149‑1 (MQ=17) gCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGcc > 8:291227/1‑149 (MQ=255) gCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGc < 2:146218/148‑1 (MQ=255) gCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGc < 2:141304/148‑1 (MQ=255) cGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCg < 6:289362/149‑1 (MQ=255) cGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGGTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGGGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCg < 5:327433/149‑1 (MQ=255) gTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTa < 7:179829/149‑1 (MQ=255) gATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAgg < 8:87578/149‑1 (MQ=255) gATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAg < 5:179408/148‑1 (MQ=255) gATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAg < 5:65860/148‑1 (MQ=255) gattcTCATGCCGCATGTCCGGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTCCTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTAcc < 8:220023/145‑1 (MQ=255) tCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGAt > 3:231497/1‑148 (MQ=255) aTGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTa < 6:290106/148‑1 (MQ=255) aTGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTa > 3:240645/1‑148 (MQ=255) ggCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAg > 2:97051/1‑149 (MQ=255) gcgcATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAg < 7:291227/148‑1 (MQ=255) aTGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAg > 2:175668/1‑128 (MQ=255) aTGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAg < 1:175668/128‑1 (MQ=255) cTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAg > 6:18847/1‑123 (MQ=255) cTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAg < 5:18847/123‑1 (MQ=255) cTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCAGATTACAGCTCGAAGCAg > 3:200791/1‑149 (MQ=255) tgttgttgtGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGGGAACCTGTGGGAGCGGGCTTGCCCGCCAAGAGGCCGGTACCGGCTTACCCGATTACAGCAAGCCGCAGt > 6:216463/1‑149 (MQ=38) gtgtgCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGTTGAACCCGCTCCTACTGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAAc < 3:274627/148‑1 (MQ=255) tgCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACtt > 7:233708/1‑148 (MQ=255) tgCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTc < 7:145593/149‑1 (MQ=255) ccAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTa > 2:182873/1‑123 (MQ=255) ccAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTa < 1:182873/123‑1 (MQ=255) cAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATc > 7:267364/1‑149 (MQ=255) cAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACACGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATc > 5:256910/1‑149 (MQ=255) aaCTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGt < 1:74148/149‑1 (MQ=255) cTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCAACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCCCGAAGAGGCCGGTACAGGCATACCCGATTACAGCACGACGCAGTCGAACGTCACATCCGTg‑g > 2:317940/1‑149 (MQ=38) cTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCCCTGCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCCGTACAGGCATAACCCAATACAGCACGACGCAGTCGAACTTCACATCGGTg‑g > 2:313119/1‑149 (MQ=38) aaaGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACg > 5:282481/1‑149 (MQ=255) aaaGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACg > 1:152427/1‑149 (MQ=255) aaaGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTACTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCGGCCAAGAGGCCGGTACAGGCTTACCCGATTTCATCTCGACGCCGTCGAACTTCACATCCGAG‑GCCACg > 6:188177/1‑149 (MQ=38) ttttGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCttt < 4:240645/149‑1 (MQ=255) tGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCGTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGt < 3:132254/148‑1 (MQ=255) ccTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGt < 2:232616/149‑1 (MQ=255) cTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTAcc < 3:195616/87‑1 (MQ=255) cTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTAcc > 4:195616/1‑87 (MQ=255) tGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGt > 5:120608/1‑148 (MQ=255) gCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAg > 2:44613/1‑149 (MQ=255) gCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAg > 1:302821/1‑149 (MQ=255) ctctTCGCGGGCAAGCCCGCTCCCACAGGTTCTCCCCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAgcg > 2:211654/1‑149 (MQ=17) tctTCGCGGGCAAGCCCGCTCCCACAGGTTCTCCCCCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAgcgc > 2:208189/1‑149 (MQ=17) tctTCGCGGGCAAGCCCGCTCCCACAGGTTCTCCCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGCACTTCACATCGGTG‑GCCACGTCTTTGTCg > 5:299639/1‑133 (MQ=17) tctTCGCGGGCAAGCCCGCTCCCACAGGTTCTCCCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCg < 6:299639/133‑1 (MQ=18) gcgGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGGCGTAGTCAACGTCAGCGCGCTc > 7:159197/1‑148 (MQ=255) gggTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGaa < 8:233708/149‑1 (MQ=255) tgTGAACCCGCTCCTACAGGGGGATAACCTGTGGGAGCGGGCTTGCCGGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAg < 6:256910/148‑1 (MQ=255) ggTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTc > 4:227392/1‑145 (MQ=255) ggTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTc < 3:227392/145‑1 (MQ=255) gTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTc > 7:264320/1‑100 (MQ=255) gTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTc < 8:264320/100‑1 (MQ=255) aaCCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGc > 7:161327/1‑146 (MQ=255) aaCCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGc < 8:161327/146‑1 (MQ=255) aaCCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCg < 4:231497/148‑1 (MQ=255) aaCCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCg > 2:79879/1‑148 (MQ=255) cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGAGGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAAc < 1:97051/149‑1 (MQ=255) cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGcc < 8:159732/145‑1 (MQ=255) cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGcc > 7:159732/1‑145 (MQ=255) tCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCAGTCAGCCGAACAGGtt > 1:111613/1‑149 (MQ=255) cTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGCGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTg < 1:79879/149‑1 (MQ=255) tACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAg > 2:152869/1‑149 (MQ=255) tACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAg > 4:116859/1‑149 (MQ=255) | GCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTG‑GCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAG > NC_002947/5257736‑5258031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |