Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	58,439	G→C	12.2%	intergenic (‑350/+338)	PP_5424 ← / ← PP_0050	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	58,439	0	G	C	12.2%	108.8 / 8.1	41	intergenic (‑350/+338)	PP_5424/PP_0050	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base C (3/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

GCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAAT  >  NC_002947/58300‑58585                                                                                                                                            |                                                                                                                                                   gccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgc                                                                                                                                           <  7:225421/149‑2 (MQ=255)   cggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgctg                                                                                                                                         <  2:212214/149‑4 (MQ=255)               tGCATCAGATCTTGATTGACTCGCGGGCCGCTTTGGATAGCCTTGCTATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACGAGCATGAGCCCGCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGAcggcgg                                                                                                                             <  1:308088/149‑1 (MQ=255)                gCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCggg                                                                                                                            >  4:178873/1‑149 (MQ=255)                gCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCggg                                                                                                                            >  6:45325/1‑149 (MQ=255)                gCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCggg                                                                                                                            >  6:289514/1‑149 (MQ=255)                 cATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCggg                                                                                                                            >  5:204616/1‑148 (MQ=255)                  aTCAGATCTTGATTGACACGCGTGCCGCATTGCATAGCCTTGCAATTGAGGGCTGCAACTACGCATTTCTCGGGCCAGGGTACCAGCATGAGCACCCGCAATATTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGAcgtccgcca                                                                                                                          >  2:186277/1‑142 (MQ=255)                     aGATCTTGATTGACACGCGTGCCGCGTTGGATAGCCTTGCAATTGAGGTCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTg                                                                                                                       <  5:139524/149‑1 (MQ=255)                          ttgattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGt                                                                                                                  >  3:47840/1‑149 (MQ=255)                          ttgattgaCACGCGTGACGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGt                                                                                                                  >  2:14967/1‑149 (MQ=255)                            gattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTc                                                                                                                 <  1:41323/148‑1 (MQ=255)                            gattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTc                                                                                                                 <  3:178873/148‑1 (MQ=255)                                          ccGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCaa                                                                                                  <  4:47840/149‑1 (MQ=255)                                          ccGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTACGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGTGGGCATTGCCAGTCGGATTCTGAACCCaa                                                                                                  <  1:186277/149‑1 (MQ=255)                                                       ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGAc                                                                                       >  3:129070/1‑147 (MQ=255)                                                       ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTa                                                                                     <  5:289514/149‑1 (MQ=255)                                                                    ggCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGAt                                                                        <  4:95891/149‑1 (MQ=255)                                                                          aaCTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGc                                                                  <  1:14967/149‑1 (MQ=255)                                                                             tGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGac                                                               >  8:34941/1‑149 (MQ=255)                                                                                 cATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGactact                                                           >  8:162529/1‑149 (MQ=255)                                                                                       tCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGAc                                                                                       >  6:242173/1‑115 (MQ=255)                                                                                       tCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGAc                                                                                       <  5:242173/115‑1 (MQ=255)                                                                                        cGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGAGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATc                                                    >  4:14441/1‑149 (MQ=255)                                                                                             cTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTa                                               <  5:45325/149‑1 (MQ=255)                                                                                                   aCCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGac                                                               >  1:75367/1‑127 (MQ=255)                                                                                                   aCCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGac                                                               <  2:75367/127‑1 (MQ=255)                                                                                                       gCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGt                                      >  2:40764/1‑148 (MQ=255)                                                                                                         aTGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc                                   <  3:14441/149‑1 (MQ=255)                                                                                                          tGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAcg                                  >  3:107235/1‑149 (MQ=255)                                                                                                             gCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAcgcg                                <  4:129070/148‑1 (MQ=255)                                                                                                                 ccGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcggt                           >  8:169507/1‑149 (MQ=255)                                                                                                                       ttttGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTaaa                     >  3:184655/1‑149 (MQ=255)                                                                                                                           tacttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGc                 >  6:14233/6‑149 (MQ=255)                                                                                                                           ggCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACgg                  <  4:184655/148‑1 (MQ=255)                                                                                                                                cagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCttt            >  7:45849/1‑149 (MQ=255)                                                                                                                                  gagGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTc           >  7:178605/1‑148 (MQ=255)                                                                                                                                       aGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAg                                                                                                 <  2:226624/57‑1 (MQ=255)                                                                                                                                       aGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAg                                                                                                 >  1:226624/1‑57 (MQ=255)                                                                                                                                       aGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGc      >  2:141055/1‑148 (MQ=255)                                                                                                                                       aGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGc      <  1:141055/148‑1 (MQ=255)                                                                                                                                            gTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAAt  <  1:40764/147‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                   GCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAAT  >  NC_002947/58300‑58585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑