Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,289,590	G→C	7.2%	L246V (CTC→GTC) ‡	PP_4663 ←	transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,289,590	0	G	C	7.2%	223.5 / 6.5	69	L246V (CTC→GTC) ‡	PP_4663	transmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (44/20);  new base C (3/2);  total (47/22)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

GGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTG  >  NC_002947/5289442‑5289734                                                                                                                                                       |                                                                                                                                                 ggCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAg                                                                                                                                                  <  8:263109/149‑1 (MQ=255)   ccGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCTATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAggg                                                                                                                                                <  4:8939/149‑1 (MQ=255)           gACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCCTCGGCGGCCac                                                                                                                                         <  6:171440/148‑2 (MQ=255)             ctatctcTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCTCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGc                                                                                                                                       <  7:163148/142‑1 (MQ=255)              gTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑AGCCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGcc                                                                                                                                      >  1:245951/1‑149 (MQ=255)              gTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGcc                                                                                                                                      >  5:327410/1‑148 (MQ=255)                gCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGAt                                                                                                                                   >  5:208855/1‑149 (MQ=255)                gCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGAt                                                                                                                                   >  3:308270/1‑149 (MQ=255)                gCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGAt                                                                                                                                   >  3:50918/1‑149 (MQ=255)                    tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCTCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGc                                                                                                                                       >  8:163148/1‑141 (MQ=255)                    tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGc                                                                                                                                       >  4:147901/1‑141 (MQ=255)                    tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGc                                                                                                                                       <  3:147901/141‑1 (MQ=255)                    tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGaa                                                                                                                                >  8:10243/1‑148 (MQ=255)                         ccTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATg                                                                                                                                  >  6:85542/1‑141 (MQ=255)                         ccTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATg                                                                                                                                  <  5:85542/141‑1 (MQ=255)                             ccGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGc                                                                                                                      >  8:71874/1‑149 (MQ=255)                              cGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCt                                                                                                                     >  3:124151/1‑149 (MQ=255)                               gaaaaTGGCCGCCTTCTTCGAAAAGCGCTTGTACT‑A‑CCTGTAGCGCTATTTCTATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCGGCTAACCGTTTATCAGCTGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCtt                                                                                                                    <  4:308270/145‑1 (MQ=255)                                  caTGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCTATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGaa                                                                                                                 <  2:245951/149‑1 (MQ=255)                                    tGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAca                                                                                                               <  6:327410/149‑1 (MQ=255)                                     ggCCGCCTTCTTCGAAAAGCGCATGGACTGA‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCCTCGGCGGCCac                                                                                                                                         >  1:187277/1‑122 (MQ=255)                                     ggCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCCTCGGCGGCCac                                                                                                                                         <  2:187277/122‑2 (MQ=255)                                                  gAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATc                                                                                                 >  8:152185/1‑149 (MQ=255)                                                   aaaaGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAATCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAg                                                                                                                                         >  5:326946/1‑108 (MQ=255)                                                   aaaaGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAATCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAg                                                                                                                                         <  6:326946/108‑1 (MQ=255)                                                   aaaaGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATcc                                                                                                >  5:35572/1‑149 (MQ=255)                                                         gcATGGACT‑C‑CCTGGCGCGCGATTTCGATATCCAGCGGGTCGCCCTGCTCAGCGCTTCGTCCGCGACCCGTTGATCAGAAGGTGGCCGCCGAGGGCGGCCAGGCCGAGGAAAAAGCCACGCTTGACCAGCAGCGCGCTGCTCCGcggcc                                                                                          >  2:243136/1‑145 (MQ=255)                                                            tGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCa                                                                                       <  5:332984/149‑1 (MQ=255)                                                               aCT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGc                                                                                     >  3:276792/1‑148 (MQ=255)                                                                     ccTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTg                                                                                >  8:13235/1‑149 (MQ=255)                                                                           gcgcGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGc                                                                          >  7:150601/1‑149 (MQ=255)                                                                               gATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAg                                                                       >  7:122700/1‑148 (MQ=255)                                                                                  ttCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGc                                                                          >  5:49635/1‑142 (MQ=255)                                                                                      atatCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGcc                                                                 >  3:106843/1‑147 (MQ=255)                                                                                       tatCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGa                                                               <  4:106843/148‑1 (MQ=255)                                                                                           cAGCGGGTCGACATGCTCAGCGCTTCGTTCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAg                                                          >  1:309327/1‑149 (MQ=255)                                                                                           cAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAg                                                          >  3:292456/1‑149 (MQ=255)                                                                                              cGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGcc                                                       <  4:292456/149‑1 (MQ=255)                                                                                                 gTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGgc                                                    <  4:124151/149‑1 (MQ=255)                                                                                                   cGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCTCTGGCCCGCCAGCATGCCGAGCAAGGCCGgcgc                                                  >  7:123403/1‑149 (MQ=255)                                                                                                         gCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                            >  4:293371/1‑149 (MQ=255)                                                                                                          cTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGgcgc                                                  >  4:37955/1‑142 (MQ=255)                                                                                                          cTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGgcgc                                                  <  3:37955/142‑1 (MQ=255)                                                                                                            cAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                         >  8:41810/1‑149 (MQ=255)                                                                                                             aGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                         >  5:273163/1‑148 (MQ=255)                                                                                                                  ttCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGa                                   >  7:279232/1‑149 (MQ=255)                                                                                                                        cGCTAACCGTGCATCAGCTGGGGGCCGCCTCGGGGGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGcccc                             <  8:122700/149‑1 (MQ=255)                                                                                                                          cTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCTATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCa                           >  8:78062/1‑149 (MQ=255)                                                                                                                           tAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAg                          >  2:240688/1‑149 (MQ=255)                                                                                                                             aCCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAgcg                        >  3:223348/1‑149 (MQ=255)                                                                                                                               cGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGcc                      >  7:177685/1‑149 (MQ=255)                                                                                                                                gTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCt                     >  6:254271/1‑149 (MQ=255)                                                                                                                                gTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCt                     >  8:22780/1‑149 (MQ=255)                                                                                                                                 ttGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                            <  6:178908/125‑1 (MQ=255)                                                                                                                                 ttGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                            >  5:178908/1‑125 (MQ=255)                                                                                                                                 ttGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGccc                              >  7:128450/1‑139 (MQ=255)                                                                                                                                 ttGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTg                    <  7:41810/149‑1 (MQ=255)                                                                                                                                     tCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCa                >  2:86446/1‑149 (MQ=255)                                                                                                                                       agcagGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAc               <  7:10243/148‑1 (MQ=255)                                                                                                                                       agcagGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAc               <  4:276792/148‑1 (MQ=255)                                                                                                                                          gcctGGCCGCCCTCGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAccc             >  1:77427/4‑147 (MQ=255)                                                                                                                                          gcatGGCCGCCCTCGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCCGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCaa           >  2:181396/4‑149 (MQ=255)                                                                                                                                            gTGGCGGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg         >  7:287045/1‑149 (MQ=255)                                                                                                                                            gTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg         >  3:112009/1‑149 (MQ=255)                                                                                                                                            gTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg         >  6:52271/1‑149 (MQ=255)                                                                                                                                            gTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg         <  7:152185/149‑1 (MQ=255)                                                                                                                                            gTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg         >  5:64138/1‑149 (MQ=255)                                                                                                                                                 cgccgAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATcc    >  4:66951/1‑149 (MQ=255)                                                                                                                                                  gccgAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCa                                                                        >  7:299827/1‑80 (MQ=255)                                                                                                                                                  gccgAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCa                                                                        <  8:299827/80‑1 (MQ=255)                                                                                                                                                   ccgTGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTg  <  6:208855/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                 GGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACT‑A‑CCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTG  >  NC_002947/5289442‑5289734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑