Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,317,521 G→C 8.4% L43V (CTG→GTG) ‡ ilvH ← acetolactate synthase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,317,5210GC 8.4% 171.6 / 5.7 60L43V (CTG→GTG) ‡ilvHacetolactate synthase small subunit
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base C (2/3);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

CCTTGACCAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTA  >  NC_002947/5317377‑5317655
                                                                                                                                                |                                                                                                                                      
ccttgaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtc                                                                                                                                    >  5:254561/1‑149 (MQ=255)
 cttgaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcg                                                                                                                                   >  8:186804/1‑149 (MQ=255)
 cttgaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcg                                                                                                                                   >  5:183229/1‑149 (MQ=255)
  ttgaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcgg                                                                                                                                  >  8:152205/1‑149 (MQ=255)
  tagaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcgg                                                                                                                                  >  5:195730/3‑149 (MQ=255)
    gaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcggg                                                                                                                                 <  3:181119/148‑1 (MQ=255)
     accAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcgggt                                                                                                                                <  3:151180/148‑1 (MQ=255)
      ccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCt                                                                                                                              >  8:36935/1‑149 (MQ=255)
      ccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCt                                                                                                                              >  6:209852/1‑149 (MQ=255)
       cAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCtt                                                                                                                             <  6:183229/149‑1 (MQ=255)
       cAACATCAGATCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGATGGTCAGCGTCAGACCCGACCCTGTCGCGTCtg                                                                                                                             <  1:112344/149‑2 (MQ=255)
              aGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTgg                                                                                                                      >  1:156316/1‑149 (MQ=255)
               gTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGgc                                                                                                                     >  2:238705/1‑149 (MQ=255)
               gTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtga                                                                                                                            <  5:219598/142‑3 (MQ=255)
               gTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtga                                                                                                                            >  6:219598/1‑140 (MQ=255)
                ttCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGgcg                                                                                                                    >  7:151771/1‑149 (MQ=255)
                   aCGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTa                                                                                                                 <  7:36935/149‑1 (MQ=255)
                   aCGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTa                                                                                                                 <  6:300673/149‑1 (MQ=255)
                     gCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAggg                                                                                                                                      >  2:304421/1‑126 (MQ=255)
                     gCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAggg                                                                                                                                      <  1:304421/126‑1 (MQ=255)
                     gCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACa                                                                                                               >  2:64001/1‑149 (MQ=255)
                          aTGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTGTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGTGTCTTCGGTTGTCGCTACAGTCAg                                                                                                          <  2:51692/149‑1 (MQ=255)
                             tgAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGc                                                                                                        <  5:175980/148‑1 (MQ=255)
                               aGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCttt                                                                                                     <  1:238705/149‑1 (MQ=255)
                               aGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCtt                                                                                                      >  3:52426/1‑148 (MQ=255)
                                       ccGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAAtgtt                                                                                              <  8:17898/148‑1 (MQ=255)
                                         gACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTa                                                                                           <  5:209852/149‑1 (MQ=255)
                                            aGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCTTGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGtt                                                                                        <  5:159657/149‑1 (MQ=255)
                                            aGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGtt                                                                                        <  1:64001/149‑1 (MQ=255)
                                              gTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTgc                                                                                      >  1:78093/1‑149 (MQ=255)
                                              gTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtg                                                                                                                             >  6:85703/1‑109 (MQ=255)
                                              gTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtg                                                                                                                             <  5:85703/110‑2 (MQ=255)
                                                  aCAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTg                                                                                  <  2:156316/149‑1 (MQ=255)
                                                  aCAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTg                                                                                  >  3:5941/1‑149 (MQ=255)
                                                    aaGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTggg                                                                                <  6:195730/149‑1 (MQ=255)
                                                        ttGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCa                                                                                                   <  1:278090/126‑1 (MQ=255)
                                                        ttGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCa                                                                                                   >  2:278090/1‑126 (MQ=255)
                                                         tGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGaa                                                                            >  7:43487/1‑148 (MQ=255)
                                                            ccACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAgg                                                                        <  4:52426/149‑1 (MQ=255)
                                                                ttCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtc                                                                                                                                    <  7:167495/85‑1 (MQ=255)
                                                                ttCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtc                                                                                                                                    >  8:167495/1‑85 (MQ=255)
                                                                ttCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGa                                                                    >  1:188624/1‑149 (MQ=255)
                                                                   gACCAGCTTGCTCAGGTTCTTGGTGATCTGTTCGATCACGTCGTCATGGCCACCGGTGGTCAGCGTCAGACGCGACAGGGGCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGAcc                                                                  <  8:116529/148‑1 (MQ=255)
                                                                        gCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCgg                                                            <  8:151771/149‑1 (MQ=255)
                                                                            gTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGAc                                                         <  2:78093/148‑1 (MQ=255)
                                                                             ttCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACaa                                                       >  1:85573/1‑149 (MQ=255)
                                                                               cAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCgg                                                            >  1:203775/1‑142 (MQ=255)
                                                                               cAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCgg                                                            <  2:203775/142‑1 (MQ=255)
                                                                                aGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACg                                                     <  4:5941/148‑1 (MQ=255)
                                                                                  gTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCAc                                                  >  3:114467/1‑149 (MQ=255)
                                                                                   ttCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTGGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCAcc                                                 <  7:186804/149‑1 (MQ=255)
                                                                                         gTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGTGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTc                                           >  5:226096/1‑149 (MQ=255)
                                                                                                   cGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCcag                                  <  4:114467/148‑1 (MQ=255)
                                                                                                         cTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAgg                           >  4:309733/1‑149 (MQ=255)
                                                                                                          ttCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAAc                                                                           <  8:225971/100‑1 (MQ=255)
                                                                                                          ttCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAAc                                                                           >  7:225971/1‑100 (MQ=255)
                                                                                                          ttCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGACGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAgg                           >  7:219694/1‑148 (MQ=255)
                                                                                                             gTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGaaa                       >  7:268708/1‑149 (MQ=255)
                                                                                                              tCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAat                      >  4:286300/1‑149 (MQ=255)
                                                                                                                aTGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAatg                     <  2:188624/148‑1 (MQ=255)
                                                                                                                aTGGCCAACGGTGGTCAGCGGCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAatga                    <  6:254561/149‑1 (MQ=255)
                                                                                                                  ggCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCcagcagc                              <  1:56271/137‑1 (MQ=255)
                                                                                                                  ggCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCcagcagc                              >  2:56271/1‑137 (MQ=255)
                                                                                                                    ccAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGa                         >  7:193797/1‑140 (MQ=255)
                                                                                                                    ccAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGa                         <  8:193797/140‑1 (MQ=255)
                                                                                                                        cGGGGGTTATCGTCAGACGCGACATGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGc            <  6:226096/149‑1 (MQ=255)
                                                                                                                              taatcGTCAGACGCGACATGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGGTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCa      <  8:219694/145‑1 (MQ=255)
                                                                                                                                 gCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATc       <  1:33671/145‑1 (MQ=255)
                                                                                                                                 gCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATc       >  2:33671/1‑145 (MQ=255)
                                                                                                                                  cGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTa  <  7:152205/149‑1 (MQ=255)
                                                                                                                                  cGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTa  >  2:977/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                      
CCTTGACCAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTA  >  NC_002947/5317377‑5317655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑