Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,317,527 G→C 8.3% P41A (CCG→GCG)  ilvH ← acetolactate synthase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,317,5270GC 8.3% 171.5 / 6.1 60P41A (CCG→GCG) ilvHacetolactate synthase small subunit
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base C (2/3);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

TTGACCAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTACGCTCCGTCTTGCT  >  NC_002947/5317379‑5317669
                                                                                                                                                    |                                                                                                                                              
ttgaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcgg                                                                                                                                                >  8:152205/1‑149 (MQ=255)
tagaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcgg                                                                                                                                                >  5:195730/3‑149 (MQ=255)
  gaccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcggg                                                                                                                                               <  3:181119/148‑1 (MQ=255)
   accAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAgggtcgggt                                                                                                                                              <  3:151180/148‑1 (MQ=255)
    ccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCt                                                                                                                                            >  8:36935/1‑149 (MQ=255)
    ccAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCt                                                                                                                                            >  6:209852/1‑149 (MQ=255)
     cAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCtt                                                                                                                                           <  6:183229/149‑1 (MQ=255)
     cAACATCAGATCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGATGGTCAGCGTCAGACCCGACCCTGTCGCGTCtg                                                                                                                                           <  1:112344/149‑2 (MQ=255)
            aGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTgg                                                                                                                                    >  1:156316/1‑149 (MQ=255)
             gTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGgc                                                                                                                                   >  2:238705/1‑149 (MQ=255)
             gTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtga                                                                                                                                          <  5:219598/142‑3 (MQ=255)
             gTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtga                                                                                                                                          >  6:219598/1‑140 (MQ=255)
              ttCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGgcg                                                                                                                                  >  7:151771/1‑149 (MQ=255)
                 aCGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTa                                                                                                                               <  6:300673/149‑1 (MQ=255)
                 aCGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTa                                                                                                                               <  7:36935/149‑1 (MQ=255)
                   gCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACa                                                                                                                             >  2:64001/1‑149 (MQ=255)
                        aTGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTGTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGTGTCTTCGGTTGTCGCTACAGTCAg                                                                                                                        <  2:51692/149‑1 (MQ=255)
                           tgAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGc                                                                                                                      <  5:175980/148‑1 (MQ=255)
                             aGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCttt                                                                                                                   <  1:238705/149‑1 (MQ=255)
                             aGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCtt                                                                                                                    >  3:52426/1‑148 (MQ=255)
                                     ccGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAAtgtt                                                                                                            <  8:17898/148‑1 (MQ=255)
                                       gACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTa                                                                                                         <  5:209852/149‑1 (MQ=255)
                                          aGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCTTGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGtt                                                                                                      <  5:159657/149‑1 (MQ=255)
                                          aGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGtt                                                                                                      <  1:64001/149‑1 (MQ=255)
                                            gTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTgc                                                                                                    >  1:78093/1‑149 (MQ=255)
                                            gTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtg                                                                                                                                           >  6:85703/1‑109 (MQ=255)
                                            gTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACCCGACCCTGTCGCGTCtg                                                                                                                                           <  5:85703/110‑2 (MQ=255)
                                                aCAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTg                                                                                                >  3:5941/1‑149 (MQ=255)
                                                aCAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTg                                                                                                <  2:156316/149‑1 (MQ=255)
                                                  aaGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTggg                                                                                              <  6:195730/149‑1 (MQ=255)
                                                      ttGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCa                                                                                                                 >  2:278090/1‑126 (MQ=255)
                                                      ttGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCa                                                                                                                 <  1:278090/126‑1 (MQ=255)
                                                       tGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGaa                                                                                          >  7:43487/1‑148 (MQ=255)
                                                          ccACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAgg                                                                                      <  4:52426/149‑1 (MQ=255)
                                                              ttCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGa                                                                                  >  1:188624/1‑149 (MQ=255)
                                                                 gACCAGCTTGCTCAGGTTCTTGGTGATCTGTTCGATCACGTCGTCATGGCCACCGGTGGTCAGCGTCAGACGCGACAGGGGCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGAcc                                                                                <  8:116529/148‑1 (MQ=255)
                                                                      gCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCgg                                                                          <  8:151771/149‑1 (MQ=255)
                                                                          gTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGAc                                                                       <  2:78093/148‑1 (MQ=255)
                                                                           ttCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACaa                                                                     >  1:85573/1‑149 (MQ=255)
                                                                             cAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCgg                                                                          <  2:203775/142‑1 (MQ=255)
                                                                             cAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCgg                                                                          >  1:203775/1‑142 (MQ=255)
                                                                              aGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACg                                                                   <  4:5941/148‑1 (MQ=255)
                                                                                gTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCAc                                                                >  3:114467/1‑149 (MQ=255)
                                                                                 ttCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTGGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCAcc                                                               <  7:186804/149‑1 (MQ=255)
                                                                                       gTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGTGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTc                                                         >  5:226096/1‑149 (MQ=255)
                                                                                                 cGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCcag                                                <  4:114467/148‑1 (MQ=255)
                                                                                                       cTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAgg                                         >  4:309733/1‑149 (MQ=255)
                                                                                                        ttCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAAc                                                                                         <  8:225971/100‑1 (MQ=255)
                                                                                                        ttCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAAc                                                                                         >  7:225971/1‑100 (MQ=255)
                                                                                                        ttCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGACGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAgg                                         >  7:219694/1‑148 (MQ=255)
                                                                                                           gTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGaaa                                     >  7:268708/1‑149 (MQ=255)
                                                                                                            tCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAat                                    >  4:286300/1‑149 (MQ=255)
                                                                                                              aTGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAatg                                   <  2:188624/148‑1 (MQ=255)
                                                                                                              aTGGCCAACGGTGGTCAGCGGCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAatga                                  <  6:254561/149‑1 (MQ=255)
                                                                                                                ggCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCcagcagc                                            <  1:56271/137‑1 (MQ=255)
                                                                                                                ggCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCcagcagc                                            >  2:56271/1‑137 (MQ=255)
                                                                                                                  ccAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGa                                       >  7:193797/1‑140 (MQ=255)
                                                                                                                  ccAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGa                                       <  8:193797/140‑1 (MQ=255)
                                                                                                                      cGGGGGTTATCGTCAGACGCGACATGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGc                          <  6:226096/149‑1 (MQ=255)
                                                                                                                            taatcGTCAGACGCGACATGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGGTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCa                    <  8:219694/145‑1 (MQ=255)
                                                                                                                               gCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATc                     >  2:33671/1‑145 (MQ=255)
                                                                                                                               gCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATc                     <  1:33671/145‑1 (MQ=255)
                                                                                                                                cGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTa                <  7:152205/149‑1 (MQ=255)
                                                                                                                                cGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTa                >  2:977/1‑149 (MQ=255)
                                                                                                                                               ggtcgggtcTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTACGCTCCGTCTTGCt  >  5:3084/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TTGACCAACATCAGTTCACGCTCGATGTGAGCGCTTTCCGACAGGTCGACAAGCTTGACCACTTCGACCAGCTTGTTCAGGTTCTTGGTGATCTGTTCGATCACTTCGTCATGGCCAACGGTGGTCAGCGTCAGACGCGACAGGGTCGGGTCTTCGGTTGGCGCTACAGTCAGGCTTTCAATGTTGTAGTTGCGCTGGGAGAACAGGCCGACCACGCGGGACAACGCACCTGGTTCGTTTTCCAGCAGCAGGGAAATGATGTGCCGCATATCAGGTACGCTCCGTCTTGCT  >  NC_002947/5317379‑5317669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑