Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,323,923 A→G 7.3% Q186R (CAG→CGG)  PP_4684 → kinase‑like domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,323,9230AG 7.3% 242.5 / 5.9 83Q186R (CAG→CGG) PP_4684kinase‑like domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (40/36);  new base G (3/3);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGTTTCACCTGCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCAT  >  NC_002947/5323776‑5324067
                                                                                                                                                   |                                                                                                                                                
ccGAGTTTCACCTGCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAg                                                                                                                                                 >  5:226637/1‑149 (MQ=255)
 cGAGTTTCACCTGCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAgg                                                                                                                                                >  6:137221/1‑149 (MQ=255)
        cACCTGCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGc                                                                                                                                         >  3:70123/1‑149 (MQ=255)
         aCCTGCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGcc                                                                                                                                        <  2:101797/149‑1 (MQ=255)
             gCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCg                                                                                                                                      >  8:156226/1‑147 (MQ=255)
                 gCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCg                                                                                                                                      <  3:277972/143‑1 (MQ=255)
                 gCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCg                                                                                                                                      >  4:277972/1‑143 (MQ=255)
                   cccACGTGAGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTCGAGCAGAACTTCGAGCAGATTCGCCCAATCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCt                                                                                                                              >  2:164937/1‑149 (MQ=255)
                    ccACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCgcagggcgtc                                                                                                                             <  5:137221/149‑7 (MQ=255)
                    ccACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCtt                                                                                                                             >  3:258525/1‑149 (MQ=255)
                    ccACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCtt                                                                                                                             <  4:230665/149‑1 (MQ=255)
                     cACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTc                                                                                                                            <  8:187113/149‑1 (MQ=255)
                         tgtgCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAgc                                                                                                                        <  7:156226/149‑1 (MQ=255)
                              cGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCGGGCCCAGGCCTgcaggg                                                                                                                                 <  1:40308/135‑3 (MQ=255)
                              cGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCGGGCCCAGGCCTgcaggg                                                                                                                                 >  2:40308/1‑133 (MQ=255)
                                      cACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGgct                                                                                                           >  7:3162/1‑149 (MQ=255)
                                         ccGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCATCTCGTCGCCCTGCTGGCCTGGTCCCGCAGCAGCTTCGAGCGCCTGCATGGgctgct                                                                                                        <  1:164937/149‑1 (MQ=255)
                                         ccGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGgctgct                                                                                                        <  5:21086/149‑1 (MQ=255)
                                               ggCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGaa                                                                                                    >  1:231128/1‑147 (MQ=255)
                                                  aCGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCGGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAgcg                                                                                               >  3:235776/1‑149 (MQ=255)
                                                  aCGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAgcg                                                                                               >  1:307168/1‑149 (MQ=255)
                                                     ccAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAgcgc                                                                                              >  2:154615/1‑147 (MQ=255)
                                                     ccAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCaa                                                                                            <  4:70123/149‑1 (MQ=255)
                                                      cAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGgctg                                                                                                          >  5:315596/1‑134 (MQ=255)
                                                      cAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGgctg                                                                                                          <  6:315596/134‑1 (MQ=255)
                                                       agagagCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAgg                                                                                          <  2:307168/149‑1 (MQ=255)
                                                        gagagCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGc                                                                                         >  7:57288/1‑149 (MQ=255)
                                                         agagCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGcc                                                                                        <  3:249397/149‑1 (MQ=255)
                                                               tGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGc                                                                                  >  6:271152/1‑149 (MQ=255)
                                                                 aTGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCt                                                                                 <  4:258525/148‑1 (MQ=255)
                                                                     ccccGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATc                                                                            >  4:295417/1‑149 (MQ=255)
                                                                     ccccGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATc                                                                            >  2:273910/1‑149 (MQ=255)
                                                                       ccGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCg                                                                          >  6:234106/1‑149 (MQ=255)
                                                                          gTGGAGCATAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCTCAGCAGCTTAGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGa                                                                       <  1:270126/149‑1 (MQ=255)
                                                                          gTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCGGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGa                                                                       <  4:235776/149‑1 (MQ=255)
                                                                          gTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGa                                                                       <  5:271152/149‑1 (MQ=255)
                                                                          gTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGa                                                                       <  3:295417/149‑1 (MQ=255)
                                                                             gAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGt                                                                     <  3:100656/148‑1 (MQ=255)
                                                                               gCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGc                                                                   >  8:230200/1‑148 (MQ=255)
                                                                                  gAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACg                                                               >  3:65637/1‑149 (MQ=255)
                                                                                     cTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTg                                                            <  1:154615/149‑1 (MQ=255)
                                                                                      ttCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTg                                                            >  6:25673/1‑148 (MQ=255)
                                                                                        cGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTg                                                            <  5:25673/146‑1 (MQ=255)
                                                                                         gAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCaa                                                                                            >  1:47914/1‑113 (MQ=255)
                                                                                         gAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCaa                                                                                            <  2:47914/113‑1 (MQ=255)
                                                                                         gAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCt                                                                                 >  3:97407/1‑124 (MQ=255)
                                                                                         gAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCt                                                                                 <  4:97407/124‑1 (MQ=255)
                                                                                             aGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCAc                                                    <  8:3162/149‑1 (MQ=255)
                                                                                             aGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCAc                                                    <  5:234106/149‑1 (MQ=255)
                                                                                                     ccATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCaa                                            >  6:165705/1‑149 (MQ=255)
                                                                                                           cTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGctgctg                                                                                                       <  3:20099/84‑1 (MQ=255)
                                                                                                           cTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGctgctg                                                                                                       >  4:20099/1‑84 (MQ=255)
                                                                                                            tCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCAcc                                     >  7:199484/1‑149 (MQ=255)
                                                                                                               gCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAgc                                                                                                                        >  1:322898/1‑63 (MQ=255)
                                                                                                               gCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAgc                                                                                                                        <  2:322898/63‑1 (MQ=255)
                                                                                                                cGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGa                                 <  8:199484/149‑1 (MQ=255)
                                                                                                                cGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGa                                 <  6:226637/149‑1 (MQ=255)
                                                                                                                 gACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGAt                                >  3:89630/1‑149 (MQ=255)
                                                                                                                   cAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATc                               >  4:263946/1‑148 (MQ=255)
                                                                                                                      aGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATg                           <  3:272199/149‑1 (MQ=255)
                                                                                                                      aGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATCGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATg                           >  4:272199/1‑149 (MQ=255)
                                                                                                                       gCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATgg                          >  6:269521/1‑149 (MQ=255)
                                                                                                                               gcagcaACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGt                                                                     <  5:83593/98‑1 (MQ=255)
                                                                                                                               gcagcaACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGt                                                                     >  6:83593/1‑98 (MQ=255)
                                                                                                                                cagcaACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAgg                     <  5:269521/145‑1 (MQ=255)
                                                                                                                                 agcaACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAggtggt                 >  3:80334/1‑148 (MQ=255)
                                                                                                                                 agcaACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAggtggt                 >  3:75936/1‑148 (MQ=255)
                                                                                                                                   caACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAggtgg                  <  6:264253/145‑1 (MQ=255)
                                                                                                                                   caACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAggtgg                  >  5:264253/1‑145 (MQ=255)
                                                                                                                                     aCTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAAc                                           <  6:176232/118‑1 (MQ=255)
                                                                                                                                     aCTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAAc                                           >  5:176232/1‑118 (MQ=255)
                                                                                                                                     aCTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCt            <  8:57288/149‑1 (MQ=255)
                                                                                                                                     aCTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCt            >  5:151109/1‑149 (MQ=255)
                                                                                                                                       tCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTc          >  7:201086/1‑149 (MQ=255)
                                                                                                                                       tCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTc          >  8:151320/1‑149 (MQ=255)
                                                                                                                                         gACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGa        >  7:245093/1‑149 (MQ=255)
                                                                                                                                          agctgcTGCGGGCCCAGGCCTGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAggtgg                  <  7:157959/133‑1 (MQ=255)
                                                                                                                                          agctgcTGCGGGCCCAGGCCTGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAggtgg                  >  8:157959/6‑138 (MQ=255)
                                                                                                                                          aCGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGAt       <  1:273910/149‑1 (MQ=255)
                                                                                                                                             cccTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGc    <  3:263946/149‑1 (MQ=255)
                                                                                                                                              ccTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTACGATTGCa   >  6:28384/1‑149 (MQ=255)
                                                                                                                                               cTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCAt  <  4:80334/149‑1 (MQ=255)
                                                                                                                                               cTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCAt  <  4:75936/149‑1 (MQ=255)
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CCGAGTTTCACCTGCAGGCCCCACGTGTGCCGATGGAGCACCCGCTGGGCACGCCAGAGAGCGTGATGGCCCCGGTGGAGCAGAACTTCGAGCAGATTCGCCCATTCCTCAGCGACAAAGCCGATCTGCAGCAACTCGACGCCCTGCAGGCCTGGGCCCGCAGCAGCTTCGAGCGCCTGCAAGGGCTGCTGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCAT  >  NC_002947/5323776‑5324067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑