Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,346,033 C→T 6.9% H477H (CAC→CAT)  acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,346,0330CT 6.9% 169.2 / 2.3 58H477H (CAC→CAT) acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/29);  new base T (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

ACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGC  >  NC_002947/5345889‑5346179
                                                                                                                                                  |                                                                                                                                                  
aCCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGc                                                                                                                                                <  4:64510/149‑1 (MQ=255)
  ccGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGc                                                                                                                                                >  2:65947/1‑147 (MQ=255)
  ccGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGc                                                                                                                                                <  1:65947/147‑1 (MQ=255)
     gCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAATAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATg                                                                                                                                           >  3:245209/1‑149 (MQ=255)
         cgcCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTc                                                                                                                                        <  1:320916/148‑1 (MQ=255)
           ccACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCATCCGCTGGTGGTCgcc                                                                                                                                     <  1:243056/149‑3 (MQ=255)
           ccACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATAAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGa                                                                                                                                      >  8:254980/1‑148 (MQ=255)
             aCCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGacac                                                                                                                                   >  2:191882/1‑149 (MQ=255)
                cAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTa                                                                                                                                >  1:279881/1‑149 (MQ=255)
                cAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTa                                                                                                                                >  6:5158/1‑149 (MQ=255)
                    cGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTc                                                                                                                            <  8:239884/149‑1 (MQ=255)
                       tGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAg                                                                                                                         <  7:254980/149‑1 (MQ=255)
                        gTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGACCCACCAGCGGATGGTCGACACCTACTTCaa                                                                                                                          <  1:191882/148‑1 (MQ=255)
                        gTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTa                                                                                                                                >  3:133799/1‑141 (MQ=255)
                        gTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTa                                                                                                                                <  4:133799/141‑1 (MQ=255)
                         ttCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGcc                                                                                                                       >  8:76822/1‑149 (MQ=255)
                         ttCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGc                                                                                                                        <  8:118404/148‑1 (MQ=255)
                                   aaCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGc                                                                                                             <  6:222159/149‑1 (MQ=255)
                                      cGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGctac                                                                                                          <  1:206977/149‑1 (MQ=255)
                                          gctgctGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTactact                                                                                                      >  7:101393/1‑149 (MQ=255)
                                              ctgGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTc                                                                                                    <  5:5158/147‑1 (MQ=255)
                                                ggACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTc                                                                                                                                        <  1:236745/109‑1 (MQ=255)
                                                ggACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTc                                                                                                                                        >  2:236745/1‑109 (MQ=255)
                                                 gACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGTCCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCAc                                                                                                  <  1:88645/147‑1 (MQ=255)
                                                 gACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTg                                                                                                <  7:76822/148‑1 (MQ=255)
                                                    gAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGa                                                                                            >  6:12616/1‑149 (MQ=255)
                                                     aaaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGccc                                                                                                                      <  7:240353/122‑1 (MQ=255)
                                                     aaaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGccc                                                                                                                      >  8:240353/1‑122 (MQ=255)
                                                      aaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACg                                                                                          >  8:233167/1‑149 (MQ=255)
                                                        aaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGt                                                                                        <  4:277021/149‑1 (MQ=255)
                                                                 tGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTcgc                                                                               >  1:86671/1‑149 (MQ=255)
                                                                 tGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTcgc                                                                               >  7:41358/1‑149 (MQ=255)
                                                                    tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACtt                                                                                                     <  6:257504/124‑1 (MQ=255)
                                                                    tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACtt                                                                                                     >  5:257504/1‑124 (MQ=255)
                                                                      gAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGc                                                                          >  2:303227/1‑149 (MQ=255)
                                                                       aaGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGcc                                                                         >  3:151803/1‑149 (MQ=255)
                                                                          gCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGTTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGAc                                                                      <  6:206521/149‑1 (MQ=255)
                                                                           ccccGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCACTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGACGTCg                                                                     >  8:232190/1‑149 (MQ=255)
                                                                            cccGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCTGTCGCGATGCCGACgg                                                                    >  8:163403/1‑149 (MQ=255)
                                                                                    cggcgTGCTGGCGATCAAACCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGactact                                                            <  7:267253/149‑1 (MQ=255)
                                                                                      gcgTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTgg                                                          <  7:65625/149‑1 (MQ=255)
                                                                                              gCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCAcc                                                    <  2:42015/147‑1 (MQ=255)
                                                                                              gCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCgg                                                  >  8:23773/1‑149 (MQ=255)
                                                                                               cGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCggg                                                 <  8:101393/149‑1 (MQ=255)
                                                                                                gATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGacta                                                              <  2:245789/135‑1 (MQ=255)
                                                                                                gATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGAATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGacta                                                              >  1:245789/1‑135 (MQ=255)
                                                                                                      aGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATcg                                          <  4:151803/149‑1 (MQ=255)
                                                                                                       gCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATcga                                         <  7:23773/149‑1 (MQ=255)
                                                                                                            cTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGAtg                                    <  2:86671/149‑1 (MQ=255)
                                                                                                               gCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGa                                 <  2:279881/149‑1 (MQ=255)
                                                                                                                   gggCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGa                                 <  7:34969/145‑1 (MQ=255)
                                                                                                                   gggCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGa                                 >  8:34969/1‑145 (MQ=255)
                                                                                                                       aGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACgtg                         >  7:68843/1‑149 (MQ=255)
                                                                                                                             gCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACgt                          <  5:217909/142‑1 (MQ=255)
                                                                                                                             gCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACgt                          >  6:217909/1‑142 (MQ=255)
                                                                                                                                         gtcGA‑CCATCCGCTGGTGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCgg        >  4:212742/3‑149 (MQ=255)
                                                                                                                                          tcGA‑CCATCCGCTGGTGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCAcc               >  7:120669/2‑141 (MQ=255)
                                                                                                                                          tcGA‑CCATCCGCTGGTGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCAcc               <  8:120669/140‑1 (MQ=255)
                                                                                                                                                caccaGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGc  >  2:36922/1‑149 (MQ=255)
                                                                                                                                                caccaGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGc  <  8:68843/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACG‑GCGA‑CCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGC  >  NC_002947/5345889‑5346179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑