Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,346,386 C→A 5.5% A595E (GCG→GAG)  acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,346,3860CA 5.5% 235.6 / 2.2 72A595E (GCG→GAG) acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (34/34);  new base A (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACG  >  NC_002947/5346241‑5346524
                                                                                                                                                 |                                                                                                                                          
ccccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgcgc                                                                                                                                          >  7:241369/1‑148 (MQ=255)
  ccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAg                                                                                                                                       >  4:272674/1‑149 (MQ=255)
  ccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAg                                                                                                                                       >  4:287780/1‑149 (MQ=255)
    aCGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAgcg                                                                                                                                     <  5:51304/149‑1 (MQ=255)
      gACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAgcgc                                                                                                                                    >  7:82972/1‑148 (MQ=255)
       aCCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTa                                                                                                                                  <  6:153341/149‑1 (MQ=255)
         cTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAcc                                                                                                                                >  1:222382/1‑149 (MQ=255)
         cTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAcc                                                                                                                                >  5:70116/1‑149 (MQ=255)
            aaGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCATGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAccc                                                                                                                               >  7:264436/1‑147 (MQ=255)
             aGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAg                                                                                                                            <  3:287780/149‑1 (MQ=255)
              gggCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGa                                                                                                                           <  3:272674/149‑1 (MQ=255)
              gggCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGa                                                                                                                           <  8:120781/149‑1 (MQ=255)
                    aGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCt                                                                                                                     >  7:256920/1‑149 (MQ=255)
                           tACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGc                                                                                                                >  3:137982/1‑147 (MQ=255)
                              gCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGc                                                                                                                >  4:28472/1‑144 (MQ=255)
                              gCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGAt                                                                                                           <  3:221277/149‑1 (MQ=255)
                                 ttCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCAt                                                                                                        >  7:246244/1‑149 (MQ=255)
                                   cGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATg                                                                                                       <  8:82972/148‑1 (MQ=255)
                                   cGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCACCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCAt                                                                                                        <  5:59056/147‑1 (MQ=255)
                                     tCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAg                                                                                                                                       >  2:130896/1‑114 (MQ=255)
                                     tCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAg                                                                                                                                       <  1:130896/114‑1 (MQ=255)
                                     tCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATgcgg                                                                                                    >  5:138974/1‑149 (MQ=255)
                                       aCGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAAGCAAGATCATgcggc                                                                                                   <  6:314819/148‑1 (MQ=255)
                                        cGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATgcggcg                                                                                                  >  2:192385/1‑148 (MQ=255)
                                        cGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGt                                                                                                 <  1:154394/149‑1 (MQ=255)
                                         gACCATGAATGGGGTGACTCCTGTTGACACGCTCAAGGCCGAGCTGCTGGCGCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCTGCGCTACCAAAGTCGCGCTCAGGCAAGATCATGCGGCGta                                                                                                <  7:98582/149‑1 (MQ=255)
                                         gACCATCAATGCCGTCACACCAGATGACACCCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGCAAATCGGCAGCTTCGCCAAGCCTGACATGATTAAGTGGGCGCCAGCGCTACCCAAGACCCGCTCAGGCAAGATCATGCGGCGta                                                                                                >  8:98582/1‑149 (MQ=255)
                                           ccATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGtata                                                                                              <  8:256920/149‑1 (MQ=255)
                                             aTGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACt                                                                                            >  7:169054/1‑149 (MQ=255)
                                                aaTGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTgcg                                                                                         >  1:15815/1‑149 (MQ=255)
                                                 aTGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTgcg                                                                                         >  3:272772/1‑148 (MQ=255)
                                                         acacCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGc                                                                                <  2:222382/149‑1 (MQ=255)
                                                          cacCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGc                                                                                <  8:241369/148‑1 (MQ=255)
                                                              aGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGc                                                                            >  6:198956/1‑148 (MQ=255)
                                                               gATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCaa                                                                          <  4:137982/149‑1 (MQ=255)
                                                                   acacGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAg                                                                      <  1:192385/149‑1 (MQ=255)
                                                                     acGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCt                                                                    >  6:248172/1‑149 (MQ=255)
                                                                     acGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCt                                                                    <  2:15815/149‑1 (MQ=255)
                                                                      cGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCTCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTg                                                                   <  8:264436/149‑1 (MQ=255)
                                                                         tCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGa                                                                 >  6:158046/1‑148 (MQ=255)
                                                                               ccGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTg                                                          >  3:141371/1‑149 (MQ=255)
                                                                               ccGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGACGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTg                                                          >  7:182251/1‑149 (MQ=255)
                                                                                 gAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTggg                                                        <  5:248172/149‑1 (MQ=255)
                                                                                       ctgGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATAc                                                  <  5:158046/149‑1 (MQ=255)
                                                                                          gCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCt                                                <  8:246244/148‑1 (MQ=255)
                                                                                          gCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCt                                                <  6:70116/148‑1 (MQ=255)
                                                                                          gCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTc                                               >  4:232533/1‑149 (MQ=255)
                                                                                           cccTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTc                                               >  1:224692/1‑148 (MQ=255)
                                                                                            ccTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGa                                             <  6:138974/149‑1 (MQ=255)
                                                                                            ccTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGa                                             >  5:12466/1‑149 (MQ=255)
                                                                                                   aGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGc                                      >  3:246233/1‑149 (MQ=255)
                                                                                                   aGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGc                                      <  8:169054/149‑1 (MQ=255)
                                                                                                   aGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGAACAAGCGGCGTATAATGAGAAAAATCGCCTGCAAAGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGc                                      >  1:262779/1‑149 (MQ=255)
                                                                                                      aaGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGaaa                                                               >  2:188984/1‑121 (MQ=255)
                                                                                                      aaGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGaaa                                                               <  1:188984/121‑1 (MQ=255)
                                                                                                      aaGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGa                                   >  7:78272/1‑149 (MQ=255)
                                                                                                       aGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGAc                                  <  6:12466/149‑1 (MQ=255)
                                                                                                       aGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGAGTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCCTCCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGAc                                  <  3:28472/149‑1 (MQ=255)
                                                                                                                gCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTg                                                                             <  4:155705/97‑1 (MQ=255)
                                                                                                                gCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTg                                                                             >  3:155705/1‑97 (MQ=255)
                                                                                                                gCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTg                         >  3:143575/1‑149 (MQ=255)
                                                                                                                 cAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTgg                        <  3:232533/149‑1 (MQ=255)
                                                                                                                    cTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGAt                                                    <  6:201221/118‑1 (MQ=255)
                                                                                                                    cTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGAt                                                    >  5:201221/1‑118 (MQ=255)
                                                                                                                     ttCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTa                      >  3:72237/1‑147 (MQ=255)
                                                                                                                       cGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAg                   <  4:272772/148‑1 (MQ=255)
                                                                                                                           aaGCCTGAGCTGATTCAGTGGGAGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCt                                                                    <  1:237177/95‑1 (MQ=255)
                                                                                                                           aaGCCTGAGCTGATTCAGTGGGAGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCt                                                                    <  1:241485/95‑1 (MQ=255)
                                                                                                                           aaGCCTGAGCTGATTCAGTGGGAGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCt                                                                    >  2:241485/1‑95 (MQ=255)
                                                                                                                           aaGCCTGAGCTGATTCAGTGGGAGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCt                                                                    >  2:237177/1‑95 (MQ=255)
                                                                                                                            aGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTg             <  2:224692/149‑1 (MQ=255)
                                                                                                                              ccTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGAt           <  8:78272/149‑1 (MQ=255)
                                                                                                                                       aTTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACg  >  1:60565/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
CCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATACCTCGACCCTGGCCGACCCGAGCGTGGTACAGGGCCTGATCGACAAACG  >  NC_002947/5346241‑5346524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑