Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,389,358 G→A 9.5% intergenic (+57/‑263) dld2 → / → PP_4738 D‑lactate dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,389,3580GA 9.5% 109.8 / 3.2 42intergenic (+57/‑263)dld2/PP_4738D‑lactate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base A (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

CTGCGAGATAGGCCTGTCGAGCCACAGCGGCATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTACACAGCCCGCCGTATGCGCGGTAAACCCGCGAACACCGG  >  NC_002947/5389211‑5389502
                                                                                                                                                   |                                                                                                                                                
cTGCGAGATAGGCCTGTCGAGCCACAGCGGCATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCTGGTTTACCCGCGAAGAGGCCgg                                                                                                                                                 <  2:17558/149‑1 (MQ=255)
     agaTAGGCCTGTCGAGCCACAGCGGCATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCctg                                                                                                                                            >  1:198157/1‑149 (MQ=255)
           gCCTGTCGCGCCACAGCGGCATCGACTACCACGGCCTGGTCTCCCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTACCGCGGTGAGATAGGGGGGGAGCGCTGCGTAGGCGCGGGTGTACCCCCGAAGCGGCCGGTccgtcgtgc                                                                                                                                       >  3:217594/1‑141 (MQ=255)
                tCGAGCCACAGCGGCATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTACCGCGTGGATATAGGTGGGGAGCGCTGCGTCGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCGGGCACATcc                                                                                                                                 >  6:177960/1‑149 (MQ=255)
                   aGCCACAGCGGCATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCccca                                                                                                                              >  3:70450/1‑149 (MQ=255)
                              cATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCa                                                                                                                   <  6:165208/149‑1 (MQ=255)
                                   aCTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTc                                                                                                                     <  7:22986/142‑1 (MQ=255)
                                   aCTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTc                                                                                                                     >  8:22986/1‑142 (MQ=255)
                                   aCTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCa                                                                                                              >  7:45468/1‑149 (MQ=255)
                                   aCTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCa                                                                                                              >  7:94160/1‑149 (MQ=255)
                                       ccACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGcca                                                                                                          >  8:288467/1‑149 (MQ=255)
                                        cACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGccac                                                                                                         >  5:247222/1‑149 (MQ=255)
                                            gCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCCCTGTCCACCGCAGCCccaacc                                                                                                     >  2:169603/1‑149 (MQ=255)
                                            gCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCACCACCCACTGTCCACCGCAGCCaccacc                                                                                                     >  1:97128/1‑149 (MQ=255)
                                                 gTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCAt                                                                                                 <  7:104922/148‑1 (MQ=255)
                                                      ccTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCAc                                                                                                                  >  2:216836/1‑126 (MQ=255)
                                                      ccTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCAc                                                                                                                  <  1:216836/126‑1 (MQ=255)
                                                        tGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCgg                                                                                           <  4:107337/147‑1 (MQ=255)
                                                        tGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCgg                                                                                           >  3:107337/1‑147 (MQ=255)
                                                          gTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCaccacc                                                                                                     >  1:304982/1‑135 (MQ=255)
                                                          gTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCaccacc                                                                                                     <  2:304982/135‑1 (MQ=255)
                                                              aCCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAAccc                                                                                   >  4:254143/1‑149 (MQ=255)
                                                                 gcgTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCt                                                                                <  2:198157/149‑1 (MQ=255)
                                                                  cgTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTc                                                                               <  7:288467/149‑1 (MQ=255)
                                                                         cGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCa                                                                        <  6:247222/149‑1 (MQ=255)
                                                                                gCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATtggt                                                                 <  3:254143/149‑1 (MQ=255)
                                                                                gCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCAGGTCCTGCTGGCCAATCCCAACCCAATGTCCACCGCAGCCACCACAGCATTAGCGGGTAAACCCGCTCCTACCCAGATaggt                                                                 >  5:165264/1‑149 (MQ=37)
                                                                                         aaCGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGcc                                                           <  3:216222/146‑1 (MQ=255)
                                                                                         aaCGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGcc                                                           >  4:212130/1‑146 (MQ=255)
                                                                                         aaCGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGcc                                                           >  4:216222/1‑146 (MQ=255)
                                                                                         aaCGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGcc                                                           <  3:212130/146‑1 (MQ=255)
                                                                                                   tataGGGGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGGCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCggtgg                                              <  5:177960/149‑1 (MQ=255)
                                                                                                    ataGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGt                                                                                         <  8:97599/105‑1 (MQ=255)
                                                                                                    ataGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGt                                                                                         >  7:97599/1‑105 (MQ=255)
                                                                                                    ataGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCggtgg                                              <  8:45468/148‑1 (MQ=255)
                                                                                                     taGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCggtgggt                                            <  4:70450/149‑1 (MQ=255)
                                                                                                           gggAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTaca                                       >  4:123135/1‑148 (MQ=255)
                                                                                                            ggAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTAcaca                                     <  3:123135/149‑1 (MQ=255)
                                                                                                              aGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTACACAGc                                   <  1:169603/149‑1 (MQ=255)
                                                                                                                          gAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTACACAGCCCGCCGTATgcg                       <  8:94160/149‑1 (MQ=255)
                                                                                                                                  ttACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTc                                                                                               <  2:129153/69‑1 (MQ=255)
                                                                                                                                  ttACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTc                                                                                               >  1:129153/1‑69 (MQ=255)
                                                                                                                                                gCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTACACAGCCCGCCGTATGCGCGGTAAACCCGCGAACACCgg  >  4:131387/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTGCGAGATAGGCCTGTCGAGCCACAGCGGCATCGACTACCACGGCCTGGTCTACCTGGTAGACCGCGTCACCCGGCCACGCAGCCTCTAACGCGTTGATATAGGTGGGGAGCGCTGCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTCCTGCTGGCCAATCCCCACCCACTGTCCACCGCAGCCACCACCGCATTCGCGGGTAAACCCGCTCCTACGCAGATTGGTGGTGCCCACACATCGGTGGGTGTACACAGCCCGCCGTATGCGCGGTAAACCCGCGAACACCGG  >  NC_002947/5389211‑5389502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑