| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,406,053 | C→G | 6.6% | A185A (GCG→GCC) | PP_4750 ← | amino acid ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,406,053 | 0 | C | G | 6.6% | 194.4 / 2.6 | 60 | A185A (GCG→GCC) | PP_4750 | amino acid ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (25/31); new base G (2/2); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCGATAC > NC_002947/5405912‑5406201 | caAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCgg < 3:255228/149‑1 (MQ=255) aGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGgtg > 6:17652/1‑148 (MQ=255) aGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGgtg > 6:12949/1‑148 (MQ=255) ccTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGa < 4:288826/149‑1 (MQ=255) cTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGAc > 7:305115/1‑149 (MQ=255) acaACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCt > 6:103417/1‑147 (MQ=255) aaCCATTGCATCCACATCGTCATCAGTTCGCTCATCGTGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAg < 7:293139/149‑1 (MQ=255) aTTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGttt < 5:12949/149‑1 (MQ=255) aTTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGttt < 5:17652/149‑1 (MQ=255) cATCCCCATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCt < 6:150411/148‑1 (MQ=255) aTCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCtgt < 2:187281/149‑1 (MQ=255) aTCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCtgt < 2:9290/149‑1 (MQ=255) agcagTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTaa > 8:104227/1‑149 (MQ=255) ttCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGcc > 5:134653/1‑116 (MQ=255) ttCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGcc < 6:134653/116‑1 (MQ=255) aTCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCt < 4:30851/148‑1 (MQ=255) gggCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGc > 6:113856/1‑119 (MQ=255) gggCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGc < 5:113856/119‑1 (MQ=255) gggCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAtt > 4:153146/1‑149 (MQ=255) ggCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGa > 4:164772/1‑123 (MQ=255) ggCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGa < 3:164772/123‑1 (MQ=255) gCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAttt > 3:8859/1‑148 (MQ=255) aaTCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCAc < 8:305115/149‑1 (MQ=255) aaTCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCAc < 3:296511/149‑1 (MQ=255) ctctGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAAc < 1:155814/149‑1 (MQ=255) tctGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGttt < 7:245542/108‑1 (MQ=255) tctGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGttt > 8:245542/1‑108 (MQ=255) tctGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACg > 6:67092/1‑149 (MQ=255) ttCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAg < 4:257/99‑1 (MQ=255) ttCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAg > 3:257/1‑99 (MQ=255) cAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAAtc < 3:153146/148‑1 (MQ=255) aaCGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAAtcgt > 7:103243/1‑149 (MQ=255) gTGGCCGACAGCCAACGAGAGAACAAGGCCATTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAAtcgtcgt < 8:70705/149‑2 (MQ=255) gTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt > 8:202942/1‑128 (MQ=255) gTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt < 7:202942/128‑1 (MQ=255) gTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGa < 7:104227/149‑1 (MQ=255) cGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCa < 4:8859/149‑1 (MQ=255) gCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCTGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGcc < 8:113325/149‑1 (MQ=255) cAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCt > 6:269530/1‑106 (MQ=255) cAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCt < 5:269530/106‑1 (MQ=255) cGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGa < 8:282330/146‑1 (MQ=255) cGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGa > 7:282330/1‑146 (MQ=255) gagagaACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTcc > 7:96569/1‑149 (MQ=255) gagaACAAGGCCACTGGCACGCTGAGCACCAGGTACACAACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCAACGGCCGAGTCCtt > 3:97767/1‑149 (MQ=255) aaCAAGGCCACTGGCACGCTGAGCAACAGGTACACCACCGCCGCCGCGGTCTAGACGCTCAGGCCCT‑GAAAGGTTTGCAGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAg > 5:157810/1‑149 (MQ=255) aaGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt > 8:255400/1‑104 (MQ=255) aaGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt < 7:255400/104‑1 (MQ=255) aaGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCAT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAg < 8:103243/149‑1 (MQ=255) ggCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGc < 5:103417/149‑1 (MQ=255) gCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCATTGGCg < 4:97767/149‑1 (MQ=255) aCGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGACTCCTTGATCAGGCCAATGGCg > 8:74055/1‑147 (MQ=255) cTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGt < 8:96569/149‑1 (MQ=255) cACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTg > 8:202488/1‑149 (MQ=255) gtctacaccGCGGCGGCGGTGTAGACGCTCAGGCCCTAGAAAGGTTTGCTGTGAACCCTGGTACGCCTGAAAGGTGATTTCCCCACCGCCAATCGTCGAGGCCACGGCCGCGTCGTTGAg > 1:57391/6‑120 (MQ=255) gtctacaccGCGGCGGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAg < 2:57391/114‑1 (MQ=255) ctacaccGCGGCGGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTgg < 6:202566/143‑1 (MQ=255) ctacaccGCGGCGGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTgg > 5:202566/4‑146 (MQ=255) caccGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGa > 6:237133/1‑71 (MQ=255) caccGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGa < 5:237133/71‑1 (MQ=255) caccGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAg > 6:15956/1‑114 (MQ=255) ccgccgcGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACAAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCGATa > 6:19921/1‑149 (MQ=255) cgccgcGGTGTAGACGCTCAGGCCGT‑GAATGGTTTGCTGTGAAACCTGGTTAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTTAGCAGGACAATGGCGTAGGTGGCTGAGGTGGGAATGGCGATAc < 6:157810/149‑1 (MQ=255) | CAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCT‑GAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCGATAC > NC_002947/5405912‑5406201 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |