Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,427,915	A→T	10.3%	L63Q (CTG→CAG)	rhlE‑I ←	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,427,915	0	A	T	10.3%	149.3 / 7.3	58	L63Q (CTG→CAG)	rhlE‑I	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base T (3/3);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

CCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTG  >  NC_002947/5427770‑5428061                                                                                                                                                  |                                                                                                                                                    cccGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGgc                                                                                                                                                  >  8:101888/1‑149 (MQ=255) cccGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGgc                                                                                                                                                  >  3:82389/1‑149 (MQ=255) cccGCCATACACAGCGTAAGTAGTCAGTGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGgc                                                                                                                                                  >  4:169162/1‑149 (MQ=255)       aTACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTg                                                                                                                                              >  3:7688/1‑147 (MQ=255)            ccGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTGCAGCGCCTGGCGCTggagggcg                                                                                                                                       <  6:165331/149‑5 (MQ=255)             cgcgTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCAcag                                                                                                                                      >  6:202353/1‑149 (MQ=255)               cgTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCAcaggc                                                                                                                                    <  7:99851/149‑1 (MQ=255)               cgTAAGTAGGCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCAcaggc                                                                                                                                    <  3:251639/149‑1 (MQ=255)                   agtagtCAGCGGCAGGTTCTCGGCGTAGTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGCCGCTGCAGCAcaggcagg                                                                                                                                 <  2:273616/148‑1 (MQ=255)                           gCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCt                                                                                                                        <  4:215360/149‑1 (MQ=255)                           gCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCt                                                                                                                        >  2:151290/1‑149 (MQ=255)                              gCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTgcagca                                                                                                                                         >  5:205428/1‑129 (MQ=255)                              gCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTgcagca                                                                                                                                         <  6:205428/129‑1 (MQ=255)                                    tctcGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTg                                                                                                               >  1:198048/1‑149 (MQ=255)                                     ctcGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGGTCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCtt                                                                                                                >  1:42576/1‑147 (MQ=255)                                     ctcGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCtt                                                                                                                <  2:42576/147‑1 (MQ=255)                                       cGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCg                                                                                                            >  2:143860/1‑149 (MQ=255)                                        ggCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGcc                                                                                                             <  6:165317/147‑1 (MQ=255)                                        ggCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGc                                                                                                              <  4:82389/146‑1 (MQ=255)                                          cGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTa                                                                                                         >  5:106574/1‑149 (MQ=255)                                              ttCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCg                                                                                                      <  2:294836/148‑1 (MQ=255)                                                   gcACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTgg                                                                                               >  8:260498/1‑149 (MQ=255)                                                     acgaagttgtCCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGc                                                                                             >  4:303457/6‑149 (MQ=255)                                                           ttgtGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCgg                                                                                        <  3:245989/148‑1 (MQ=255)                                                              tgCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCAt                                                                                    <  1:143860/149‑1 (MQ=255)                                                              tgCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCAt                                                                                    <  2:198048/149‑1 (MQ=255)                                                                cACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATc                                                                                   <  3:169162/148‑1 (MQ=255)                                                                 aCCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCg                                                                                                      <  2:279735/129‑1 (MQ=255)                                                                 aCCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCg                                                                                                      >  1:279735/1‑129 (MQ=255)                                                                        cGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGcgg                                                                          >  5:150033/1‑149 (MQ=255)                                                                         ggCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTcgcg                                                                           <  7:101888/147‑1 (MQ=255)                                                                            cAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTgg                                                                                               >  4:158445/1‑124 (MQ=255)                                                                            cAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTgg                                                                                               <  3:158445/124‑1 (MQ=255)                                                                            cAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGcggccgg                                                                      >  5:241119/1‑149 (MQ=255)                                                                              gCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCggccggcc                                                                    <  4:7688/149‑1 (MQ=255)                                                                                                gCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCt                                                  <  3:303457/149‑1 (MQ=255)                                                                                                   ccAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTg                                               <  5:202353/149‑1 (MQ=255)                                                                                                    cAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTgg                                              <  1:151290/149‑1 (MQ=255)                                                                                                     aaGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGc                                             >  3:309749/1‑149 (MQ=255)                                                                                                             gATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACcg                                     >  1:162201/1‑149 (MQ=255)                                                                                                               tCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACcggg                                   <  5:114118/149‑1 (MQ=255)                                                                                                                   gTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACcggggtcg                               <  7:260498/149‑1 (MQ=255)                                                                                                                            cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCggccggcc                                                                    >  8:94309/1‑103 (MQ=255)                                                                                                                            cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCggccggcc                                                                    <  7:94309/103‑1 (MQ=255)                                                                                                                            cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTg                                               <  5:11026/124‑1 (MQ=255)                                                                                                                            cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTg                                               >  6:11026/1‑124 (MQ=255)                                                                                                                            cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGgtggtg                       >  1:247104/1‑148 (MQ=255)                                                                                                                            cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACAGGGGTCGGGgtggtg                       >  4:270246/1‑148 (MQ=255)                                                                                                                                    tgtgctgcAGCGCCTGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGATACTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAg               >  1:257583/8‑149 (MQ=255)                                                                                                                                     gtgctgcAGCGCCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGtt             >  2:293281/7‑149 (MQ=255)                                                                                                                                         tgcAGCGCCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATgg                                                    >  7:92172/3‑106 (MQ=255)                                                                                                                                         tgcAGCGCCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATgg                                                    <  8:92172/104‑1 (MQ=255)                                                                                                                                         tCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTtgctg         >  8:243783/1‑149 (MQ=255)                                                                                                                                             gcgcCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGc     <  1:293281/149‑1 (MQ=255)                                                                                                                                               gcCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGt   <  7:243783/149‑1 (MQ=255)                                                                                                                                                ccAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTg  <  6:106574/149‑1 (MQ=255)                                                                                                                                                 cAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGggtggt                        <  2:239475/126‑1 (MQ=255)                                                                                                                                                 cAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGggtggt                        >  1:239475/1‑126 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CCCGCCATACACCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTG  >  NC_002947/5427770‑5428061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑