Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,923 C→G 7.5% L60L (CTG→CTC)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,9230CG 7.5% 169.0 / 3.4 53L60L (CTG→CTC) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (22/27);  new base G (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGC  >  NC_002947/5427781‑5428070
                                                                                                                                              |                                                                                                                                                    
ccGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTGCAGCGCCTGGCGCTggagggcg                                                                                                                                                <  6:165331/149‑5 (MQ=255)
 cgcgTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCAcag                                                                                                                                               >  6:202353/1‑149 (MQ=255)
   cgTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCAcaggc                                                                                                                                             <  7:99851/149‑1 (MQ=255)
   cgTAAGTAGGCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCAcaggc                                                                                                                                             <  3:251639/149‑1 (MQ=255)
       agtagtCAGCGGCAGGTTCTCGGCGTAGTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGCCGCTGCAGCAcaggcagg                                                                                                                                          <  2:273616/148‑1 (MQ=255)
               gCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCt                                                                                                                                 >  2:151290/1‑149 (MQ=255)
               gCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCt                                                                                                                                 <  4:215360/149‑1 (MQ=255)
                  gCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTgcagca                                                                                                                                                  <  6:205428/129‑1 (MQ=255)
                  gCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTgcagca                                                                                                                                                  >  5:205428/1‑129 (MQ=255)
                        tctcGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTg                                                                                                                        >  1:198048/1‑149 (MQ=255)
                         ctcGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGGTCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCtt                                                                                                                         >  1:42576/1‑147 (MQ=255)
                         ctcGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCtt                                                                                                                         <  2:42576/147‑1 (MQ=255)
                           cGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCg                                                                                                                     >  2:143860/1‑149 (MQ=255)
                            ggCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGcc                                                                                                                      <  6:165317/147‑1 (MQ=255)
                            ggCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGc                                                                                                                       <  4:82389/146‑1 (MQ=255)
                              cGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTa                                                                                                                  >  5:106574/1‑149 (MQ=255)
                                  ttCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCg                                                                                                               <  2:294836/148‑1 (MQ=255)
                                       gcACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTgg                                                                                                        >  8:260498/1‑149 (MQ=255)
                                         acgaagttgtCCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGc                                                                                                      >  4:303457/6‑149 (MQ=255)
                                               ttgtGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCgg                                                                                                 <  3:245989/148‑1 (MQ=255)
                                                  tgCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCAt                                                                                             <  1:143860/149‑1 (MQ=255)
                                                  tgCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCAt                                                                                             <  2:198048/149‑1 (MQ=255)
                                                    cACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATc                                                                                            <  3:169162/148‑1 (MQ=255)
                                                     aCCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCg                                                                                                               <  2:279735/129‑1 (MQ=255)
                                                     aCCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCg                                                                                                               >  1:279735/1‑129 (MQ=255)
                                                            cGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGcgg                                                                                   >  5:150033/1‑149 (MQ=255)
                                                             ggCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTcgcg                                                                                    <  7:101888/147‑1 (MQ=255)
                                                                cAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTgg                                                                                                        <  3:158445/124‑1 (MQ=255)
                                                                cAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTgg                                                                                                        >  4:158445/1‑124 (MQ=255)
                                                                cAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGcggccgg                                                                               >  5:241119/1‑149 (MQ=255)
                                                                  gCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCggccggcc                                                                             <  4:7688/149‑1 (MQ=255)
                                                                                    gCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCt                                                           <  3:303457/149‑1 (MQ=255)
                                                                                       ccAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTg                                                        <  5:202353/149‑1 (MQ=255)
                                                                                        cAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTgg                                                       <  1:151290/149‑1 (MQ=255)
                                                                                         aaGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGc                                                      >  3:309749/1‑149 (MQ=255)
                                                                                                 gATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACcg                                              >  1:162201/1‑149 (MQ=255)
                                                                                                   tCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACcggg                                            <  5:114118/149‑1 (MQ=255)
                                                                                                       gTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACcggggtcg                                        <  7:260498/149‑1 (MQ=255)
                                                                                                                cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCggccggcc                                                                             >  8:94309/1‑103 (MQ=255)
                                                                                                                cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCggccggcc                                                                             <  7:94309/103‑1 (MQ=255)
                                                                                                                cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTg                                                        <  5:11026/124‑1 (MQ=255)
                                                                                                                cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTg                                                        >  6:11026/1‑124 (MQ=255)
                                                                                                                cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGgtggtg                                >  1:247104/1‑148 (MQ=255)
                                                                                                                cACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACAGGGGTCGGGgtggtg                                >  4:270246/1‑148 (MQ=255)
                                                                                                                        tgtgctgcAGCGCCTGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGATACTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAg                        >  1:257583/8‑149 (MQ=255)
                                                                                                                         gtgctgcAGCGCCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGtt                      >  2:293281/7‑149 (MQ=255)
                                                                                                                             tgcAGCGCCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATgg                                                             >  7:92172/3‑106 (MQ=255)
                                                                                                                             tgcAGCGCCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATgg                                                             <  8:92172/104‑1 (MQ=255)
                                                                                                                             tCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTtgctg                  >  8:243783/1‑149 (MQ=255)
                                                                                                                                 gcgcCTGGCGCTGGAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGc              <  1:293281/149‑1 (MQ=255)
                                                                                                                                   gcCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGt            <  7:243783/149‑1 (MQ=255)
                                                                                                                                    ccAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTg           <  6:106574/149‑1 (MQ=255)
                                                                                                                                     cAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGggtggt                                 <  2:239475/126‑1 (MQ=255)
                                                                                                                                     cAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGggtggt                                 >  1:239475/1‑126 (MQ=255)
                                                                                                                                       ggCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGt            >  7:72278/1‑145 (MQ=255)
                                                                                                                                       ggCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGt            <  8:72278/145‑1 (MQ=255)
                                                                                                                                              cagcaCAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTGCGCagcagc  <  7:205495/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCGCGTAAGTAGTCAGCGGCAGGTTCTCGGCGTATTCGCGCACGTTGTTGTGCACCTGCTCGGCCAGCTCGCGAGTGGGTACCAGCACCAAGGCGCGGATCGAGTTGCTGGCCACCTTCTCGCCCTCCAGCGCCAGGCGCTGCAGCACAGGCAGGGCAAAGCCTGCGGTCTTGCCGGTACCGGT‑CTGGGCTGCGGCCATCAGGTCGCGGCCGGCCAGCACGGCGGGGATGGCTTTGGCCTGCACCGGGGTCGGGGTGGTGTAGTCCAGTTGCTGCAGCGTGCGCAGCAGC  >  NC_002947/5427781‑5428070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑