Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,464,862	A→G	8.5%	Y86H (TAC→CAC)	lipB ←	octanoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,464,862	0	A	G	8.5%	164.6 / 4.7	59	Y86H (TAC→CAC)	lipB	octanoyltransferase
Reads supporting (aligned to +/- strand):  ref base A (28/26);  new base G (2/3);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTC  >  NC_002947/5464717‑5465009                                                                                                                                                   |                                                                                                                                                    gCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGc                                                                                                                                                  <  2:48117/149‑1 (MQ=255) gCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGc                                                                                                                                                  <  2:43429/149‑1 (MQ=255)  cTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGcc                                                                                                                                                 <  6:305161/149‑1 (MQ=255)  cTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGcc                                                                                                                                                 <  6:300423/149‑1 (MQ=255)      gTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCAAGCACGCCAAAGCCC‑AGCCGGCGCCCATCAAGCAGCAGGTAGGccaccc                                                                                                                                             >  7:129233/1‑148 (MQ=255)       tCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAg                                                                                                                                            <  3:37502/149‑1 (MQ=255)            aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                       <  8:38678/149‑2 (MQ=255)               gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGAACAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCgg                                                                                                                                    >  3:73960/1‑149 (MQ=255)                    ccGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCa                                                                                                                               >  2:143881/1‑149 (MQ=255)                                 cTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCt                                                                                                                  >  3:110944/1‑149 (MQ=255)                                    ggCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                                 <  1:143881/147‑1 (MQ=255)                                    ggCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                                 <  4:110944/147‑1 (MQ=255)                                           gCCTGAACAGCATAGCTGGCGATCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCATCTCACGCACGCCAAATCCCTTGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCcgccgc                                                                                                         <  1:100733/149‑1 (MQ=255)                                           gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                        >  7:169893/1‑149 (MQ=255)                                                   aGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCACGGTCGGTCt                                                                                                >  2:278832/1‑149 (MQ=255)                                                     cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGGGACCTGGCCGCCGCGGTCGGTCTGc                                                                                              <  2:251371/149‑1 (MQ=255)                                                     cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGCTAGGTGACATGGCCGCCGCGGTCGGTCTg                                                                                               >  5:26183/1‑148 (MQ=255)                                                     cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGAAACGGCTCACCACCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTa                                                                                                                                                     >  3:181594/1‑94 (MQ=255)                                                        aGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATgg                                                                                                                            >  2:289372/1‑116 (MQ=255)                                                        aGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATgg                                                                                                                            <  1:289372/116‑1 (MQ=255)                                                                     gTCGATGAGGGCCAGCTCGATACGGCTGATCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGc                                                                              <  4:73960/149‑1 (MQ=255)                                                                     gTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGc                                                                              >  5:295527/1‑149 (MQ=255)                                                                       cGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGccc                                                                            <  7:295930/149‑1 (MQ=255)                                                                          tGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                       <  2:292072/87‑2 (MQ=38)                                                                          tGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                       >  1:292072/1‑86 (MQ=38)                                                                              ggCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCAc                                                                       >  2:177535/1‑147 (MQ=255)                                                                                  aGCTAGATACGGCTGACCAGCTCACGCACGACAAAGCCC‑AGACGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCACGGGGCCCTGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGAACCACCGGAATGTCGCCCGGCACcagcag                                                                 >  2:276575/1‑149 (MQ=255)                                                                                    cTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGt                                                               >  3:127773/1‑149 (MQ=255)                                                                                     tCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTg                                                              >  2:231654/1‑149 (MQ=255)                                                                                       gATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGa                                                                                     >  3:289027/1‑124 (MQ=255)                                                                                       gATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGa                                                                                     <  4:289027/124‑1 (MQ=255)                                                                                         tACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgg                                                          <  5:128188/149‑4 (MQ=255)                                                                                            ggCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGcc                                                       >  8:109995/1‑149 (MQ=255)                                                                                                   cAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCgg                                                >  2:122235/1‑149 (MQ=255)                                                                                                    aGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGc                                               <  6:295527/149‑1 (MQ=255)                                                                                                    aGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGAACCAGCAGGTGCTCGGCCTTGCCGGc                                               >  1:279886/1‑149 (MQ=255)                                                                                                         acgcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGcc                                                  <  5:305943/141‑1 (MQ=255)                                                                                                         acgcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGcc                                                  >  6:305943/1‑141 (MQ=255)                                                                                                           gcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                             <  7:292860/112‑1 (MQ=255)                                                                                                           gcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                             >  8:292860/1‑112 (MQ=255)                                                                                                             acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGc                                                   >  2:24288/1‑136 (MQ=255)                                                                                                             acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGc                                                   <  1:24288/136‑1 (MQ=255)                                                                                                             acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTg                                      >  3:164085/1‑149 (MQ=255)                                                                                                               gcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                    <  8:169893/149‑1 (MQ=255)                                                                                                                ccAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                   <  1:177535/149‑1 (MQ=255)                                                                                                                 cAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                   >  3:201384/1‑148 (MQ=255)                                                                                                                 cAAAGACC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                   >  5:98257/1‑148 (MQ=255)                                                                                                                   aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGTGTGaa                                <  4:164085/149‑1 (MQ=255)                                                                                                                   aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGaa                                <  7:109995/149‑1 (MQ=255)                                                                                                                   aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGaa                                <  1:231654/149‑1 (MQ=255)                                                                                                                     gCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGa                              >  1:69800/1‑149 (MQ=255)                                                                                                                      ccc‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGAc                             <  1:160928/149‑1 (MQ=255)                                                                                                                        c‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCg                           >  7:302156/1‑149 (MQ=255)                                                                                                                                       cgggcagctgGTGGCCTACCTGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                    <  4:71819/123‑1 (MQ=255)                                                                                                                                       cgggcagctgGTGGCCTACCTGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                    >  3:71819/4‑126 (MQ=255)                                                                                                                                         agcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGc                                           >  8:45538/1‑117 (MQ=255)                                                                                                                                         agcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGc                                           <  7:45538/117‑1 (MQ=255)                                                                                                                                              cagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGa      <  2:279886/149‑1 (MQ=255)                                                                                                                                               agGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAt     >  1:11504/1‑149 (MQ=255)                                                                                                                                                 gTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAttt   >  6:171605/1‑149 (MQ=255)                                                                                                                                                  gaGGCCACCATCTGCCCGGGGACATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTc  <  8:129233/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTC  >  NC_002947/5464717‑5465009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑