Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,464,864	G→C	8.3%	A85G (GCC→GGC)	lipB ←	octanoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,464,864	0	G	C	8.3%	179.9 / 7.1	60	A85G (GCC→GGC)	lipB	octanoyltransferase
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base C (2/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

GCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCAT  >  NC_002947/5464717‑5465011                                                                                                                                                     |                                                                                                                                                    gCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGc                                                                                                                                                    <  2:48117/149‑1 (MQ=255) gCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGc                                                                                                                                                    <  2:43429/149‑1 (MQ=255)  cTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGcc                                                                                                                                                   <  6:300423/149‑1 (MQ=255)  cTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGcc                                                                                                                                                   <  6:305161/149‑1 (MQ=255)      gTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCAAGCACGCCAAAGCCC‑AGCCGGCGCCCATCAAGCAGCAGGTAGGccaccc                                                                                                                                               >  7:129233/1‑148 (MQ=255)       tCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAg                                                                                                                                              <  3:37502/149‑1 (MQ=255)            aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                         <  8:38678/149‑2 (MQ=255)               gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGAACAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCgg                                                                                                                                      >  3:73960/1‑149 (MQ=255)                    ccGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCa                                                                                                                                 >  2:143881/1‑149 (MQ=255)                                 cTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCt                                                                                                                    >  3:110944/1‑149 (MQ=255)                                    ggCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                                   <  1:143881/147‑1 (MQ=255)                                    ggCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                                   <  4:110944/147‑1 (MQ=255)                                           gCCTGAACAGCATAGCTGGCGATCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCATCTCACGCACGCCAAATCCCTTGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCcgccgc                                                                                                           <  1:100733/149‑1 (MQ=255)                                           gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                          >  7:169893/1‑149 (MQ=255)                                                   aGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCACGGTCGGTCt                                                                                                  >  2:278832/1‑149 (MQ=255)                                                     cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGGGACCTGGCCGCCGCGGTCGGTCTGc                                                                                                <  2:251371/149‑1 (MQ=255)                                                     cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGCTAGGTGACATGGCCGCCGCGGTCGGTCTg                                                                                                 >  5:26183/1‑148 (MQ=255)                                                        aGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATgg                                                                                                                              <  1:289372/116‑1 (MQ=255)                                                        aGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATgg                                                                                                                              >  2:289372/1‑116 (MQ=255)                                                                     gTCGATGAGGGCCAGCTCGATACGGCTGATCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGc                                                                                <  4:73960/149‑1 (MQ=255)                                                                     gTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGc                                                                                >  5:295527/1‑149 (MQ=255)                                                                       cGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGccc                                                                              <  7:295930/149‑1 (MQ=255)                                                                          tGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                         <  2:292072/87‑2 (MQ=38)                                                                          tGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                         >  1:292072/1‑86 (MQ=38)                                                                              ggCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCAc                                                                         >  2:177535/1‑147 (MQ=255)                                                                                  aGCTAGATACGGCTGACCAGCTCACGCACGACAAAGCCC‑AGACGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCACGGGGCCCTGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGAACCACCGGAATGTCGCCCGGCACcagcag                                                                   >  2:276575/1‑149 (MQ=255)                                                                                    cTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGt                                                                 >  3:127773/1‑149 (MQ=255)                                                                                     tCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTg                                                                >  2:231654/1‑149 (MQ=255)                                                                                       gATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGa                                                                                       >  3:289027/1‑124 (MQ=255)                                                                                       gATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGa                                                                                       <  4:289027/124‑1 (MQ=255)                                                                                         tACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgg                                                            <  5:128188/149‑4 (MQ=255)                                                                                            ggCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGcc                                                         >  8:109995/1‑149 (MQ=255)                                                                                                   cAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCgg                                                  >  2:122235/1‑149 (MQ=255)                                                                                                    aGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGc                                                 <  6:295527/149‑1 (MQ=255)                                                                                                    aGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGAACCAGCAGGTGCTCGGCCTTGCCGGc                                                 >  1:279886/1‑149 (MQ=255)                                                                                                         acgcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGcc                                                    >  6:305943/1‑141 (MQ=255)                                                                                                         acgcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGcc                                                    <  5:305943/141‑1 (MQ=255)                                                                                                           gcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                               <  7:292860/112‑1 (MQ=255)                                                                                                           gcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                               >  8:292860/1‑112 (MQ=255)                                                                                                             acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGc                                                     <  1:24288/136‑1 (MQ=255)                                                                                                             acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGc                                                     >  2:24288/1‑136 (MQ=255)                                                                                                             acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTg                                        >  3:164085/1‑149 (MQ=255)                                                                                                               gcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                      <  8:169893/149‑1 (MQ=255)                                                                                                                ccAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                     <  1:177535/149‑1 (MQ=255)                                                                                                                 cAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                     >  3:201384/1‑148 (MQ=255)                                                                                                                 cAAAGACC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                     >  5:98257/1‑148 (MQ=255)                                                                                                                   aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGTGTGaa                                  <  4:164085/149‑1 (MQ=255)                                                                                                                   aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGaa                                  <  7:109995/149‑1 (MQ=255)                                                                                                                   aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGaa                                  <  1:231654/149‑1 (MQ=255)                                                                                                                     gCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGa                                >  1:69800/1‑149 (MQ=255)                                                                                                                      ccc‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGAc                               <  1:160928/149‑1 (MQ=255)                                                                                                                        c‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCg                             >  7:302156/1‑149 (MQ=255)                                                                                                                                       cgggcagctgGTGGCCTACCTGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                      <  4:71819/123‑1 (MQ=255)                                                                                                                                       cgggcagctgGTGGCCTACCTGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                      >  3:71819/4‑126 (MQ=255)                                                                                                                                         agcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGc                                             >  8:45538/1‑117 (MQ=255)                                                                                                                                         agcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGc                                             <  7:45538/117‑1 (MQ=255)                                                                                                                                              cagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGa        <  2:279886/149‑1 (MQ=255)                                                                                                                                               agGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAt       >  1:11504/1‑149 (MQ=255)                                                                                                                                                 gTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAttt     >  6:171605/1‑149 (MQ=255)                                                                                                                                                  gaGGCCACCATCTGCCCGGGGACATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTc    <  8:129233/148‑1 (MQ=255)                                                                                                                                                    ggCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCg                  <  6:153576/133‑1 (MQ=255)                                                                                                                                                    ggCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCg                  >  5:153576/1‑133 (MQ=255)                                                                                                                                                    ggCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCAt  <  8:302156/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCAT  >  NC_002947/5464717‑5465011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑