Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,464,866	C→T	8.5%	V84V (GTG→GTA)	lipB ←	octanoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,464,866	0	C	T	8.5%	162.5 / 3.8	58	V84V (GTG→GTA)	lipB	octanoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base T (2/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCATCCT  >  NC_002947/5464718‑5465014                                                                                                                                                      |                                                                                                                                                     cTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGcc                                                                                                                                                      <  6:305161/149‑1 (MQ=255) cTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGcc                                                                                                                                                      <  6:300423/149‑1 (MQ=255)     gTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCAAGCACGCCAAAGCCC‑AGCCGGCGCCCATCAAGCAGCAGGTAGGccaccc                                                                                                                                                  >  7:129233/1‑148 (MQ=255)      tCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAg                                                                                                                                                 <  3:37502/149‑1 (MQ=255)           aTAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                            <  8:38678/149‑2 (MQ=255)              gACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGAACAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCgg                                                                                                                                         >  3:73960/1‑149 (MQ=255)                   ccGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCa                                                                                                                                    >  2:143881/1‑149 (MQ=255)                                cTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCt                                                                                                                       >  3:110944/1‑149 (MQ=255)                                   ggCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                                      <  1:143881/147‑1 (MQ=255)                                   ggCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTg                                                                                                                      <  4:110944/147‑1 (MQ=255)                                          gCCTGAACAGCATAGCTGGCGATCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCATCTCACGCACGCCAAATCCCTTGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCcgccgc                                                                                                              <  1:100733/149‑1 (MQ=255)                                          gCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCcgcg                                                                                                             >  7:169893/1‑149 (MQ=255)                                                  aGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCACGGTCGGTCt                                                                                                     >  2:278832/1‑149 (MQ=255)                                                    cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGGGACCTGGCCGCCGCGGTCGGTCTGc                                                                                                   <  2:251371/149‑1 (MQ=255)                                                    cATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGCTAGGTGACATGGCCGCCGCGGTCGGTCTg                                                                                                    >  5:26183/1‑148 (MQ=255)                                                       aGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATgg                                                                                                                                 <  1:289372/116‑1 (MQ=255)                                                       aGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATgg                                                                                                                                 >  2:289372/1‑116 (MQ=255)                                                                    gTCGATGAGGGCCAGCTCGATACGGCTGATCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGc                                                                                   <  4:73960/149‑1 (MQ=255)                                                                    gTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGc                                                                                   >  5:295527/1‑149 (MQ=255)                                                                      cGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGccc                                                                                 <  7:295930/149‑1 (MQ=255)                                                                         tGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                            <  2:292072/87‑2 (MQ=38)                                                                         tGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCTGGTGGCCTACCtgctgct                                                                                                                                            >  1:292072/1‑86 (MQ=38)                                                                             ggCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCAc                                                                            >  2:177535/1‑147 (MQ=255)                                                                                 aGCTAGATACGGCTGACCAGCTCACGCACGACAAAGCCC‑AGACGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCACGGGGCCCTGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGAACCACCGGAATGTCGCCCGGCACcagcag                                                                      >  2:276575/1‑149 (MQ=255)                                                                                   cTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGt                                                                    >  3:127773/1‑149 (MQ=255)                                                                                    tCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTg                                                                   >  2:231654/1‑149 (MQ=255)                                                                                      gATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGa                                                                                          >  3:289027/1‑124 (MQ=255)                                                                                      gATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGa                                                                                          <  4:289027/124‑1 (MQ=255)                                                                                        tACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCTGCTGGTGccgg                                                               <  5:128188/149‑4 (MQ=255)                                                                                           ggCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGcc                                                            >  8:109995/1‑149 (MQ=255)                                                                                                  cAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCgg                                                     >  2:122235/1‑149 (MQ=255)                                                                                                   aGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGc                                                    <  6:295527/149‑1 (MQ=255)                                                                                                   aGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGAACCAGCAGGTGCTCGGCCTTGCCGGc                                                    >  1:279886/1‑149 (MQ=255)                                                                                                        acgcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGcc                                                       <  5:305943/141‑1 (MQ=255)                                                                                                        acgcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGcc                                                       >  6:305943/1‑141 (MQ=255)                                                                                                          gcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                                  >  8:292860/1‑112 (MQ=255)                                                                                                          gcacgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGcc                                                                                  <  7:292860/112‑1 (MQ=255)                                                                                                            acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGc                                                        <  1:24288/136‑1 (MQ=255)                                                                                                            acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGc                                                        >  2:24288/1‑136 (MQ=255)                                                                                                            acgcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTg                                           >  3:164085/1‑149 (MQ=255)                                                                                                              gcCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                         <  8:169893/149‑1 (MQ=255)                                                                                                               ccAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                        <  1:177535/149‑1 (MQ=255)                                                                                                                cAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                        >  3:201384/1‑148 (MQ=255)                                                                                                                cAAAGACC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGt                                        >  5:98257/1‑148 (MQ=255)                                                                                                                  aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGTGTGaa                                     <  4:164085/149‑1 (MQ=255)                                                                                                                  aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGaa                                     <  1:231654/149‑1 (MQ=255)                                                                                                                  aaGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGaa                                     <  7:109995/149‑1 (MQ=255)                                                                                                                    gCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGa                                   >  1:69800/1‑149 (MQ=255)                                                                                                                     ccc‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGAc                                  <  1:160928/149‑1 (MQ=255)                                                                                                                       c‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCg                                >  7:302156/1‑149 (MQ=255)                                                                                                                                      cgggcagctgGTGGCCTACCTGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                         <  4:71819/123‑1 (MQ=255)                                                                                                                                      cgggcagctgGTGGCCTACCTGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTggg                                         >  3:71819/4‑126 (MQ=255)                                                                                                                                        agcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGc                                                <  7:45538/117‑1 (MQ=255)                                                                                                                                        agcagcagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGc                                                >  8:45538/1‑117 (MQ=255)                                                                                                                                             cagGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGa           <  2:279886/149‑1 (MQ=255)                                                                                                                                              agGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAt          >  1:11504/1‑149 (MQ=255)                                                                                                                                                gTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGAttt        >  6:171605/1‑149 (MQ=255)                                                                                                                                                 gaGGCCACCATCTGCCCGGGGACATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTc       <  8:129233/148‑1 (MQ=255)                                                                                                                                                   gtccaccaGCTGCCCGGCGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCAt     <  6:26183/147‑1 (MQ=255)                                                                                                                                                   ggCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCg                     <  6:153576/133‑1 (MQ=255)                                                                                                                                                   ggCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCg                     >  5:153576/1‑133 (MQ=255)                                                                                                                                                   ggCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCAt     <  8:302156/149‑1 (MQ=255)                                                                                                                                                     ccaccaGCTGCCCGGGGCCATGGTAGGTGACCTGCCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCATcc   >  3:132504/1‑149 (MQ=255)                                                                                                                                                      caccaGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCATCCt  >  8:180230/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CTCCGTCGACATAGACACCCGGGGCATCGGGCTTGGCAGACGCCTGAACAGCATAGCTGGCGAGCAGGTCGATGAGGGCCAGCTCGATACGGCTGACCAGCTCACGCACGCCAAAGCCC‑AGCCGGCGCACATCCAGCAGCAGGTAGGCCACCAGCTGCCCGGGGCCATGGTAGGTGACCTGGCCGCCGCGGTCGGTCTGCACCACCGGAATGTCGCCCGGCACCAGCAGGTGCTCGGCCTTGCCGGCCTGCCCCTGGGTGAAGACCGCAGGGTGCTCGACCAGCCAGATTTCATCCT  >  NC_002947/5464718‑5465014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑