Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,470,163 A→T 7.4% V581D (GTT→GAT)  mrdA‑II ← transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,470,1630AT 7.4% 151.4 / 2.6 54V581D (GTT→GAT) mrdA‑IItranspeptidase
Reads supporting (aligned to +/- strand):  ref base A (24/26);  new base T (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01

ACAGGGCCGATTCCTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGG  >  NC_002947/5470017‑5470306
                                                                                                                                                  |                                                                                                                                               
acaGGTCCGATTCCTGCGTAACTGTGGCTGGCGCGAACTCGGGTTTTAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGTCGCGGCGACACGCGAGCCTGACTCACCGTTCTCGACCATCAACGaaaa                                                                                                                                                <  2:148947/148‑2 (MQ=255)
acaGGGCCGATTCCTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGACCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAAcc                                                                                                                                               <  7:167211/149‑1 (MQ=255)
 caGGGCCGATTCCTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCGCCGTTCTCGACCATCACCGAAACCa                                                                                                                                              >  8:168259/1‑149 (MQ=255)
 caGGGCCGATTCCTGGGTAACGGGGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCa                                                                                                                                              <  2:103968/149‑1 (MQ=255)
    ggCCGATTCCTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGa                                                                                                                                           >  2:67629/1‑149 (MQ=255)
          ttCCTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCg                                                                                                                                     <  1:170446/149‑1 (MQ=255)
            ccTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCgg                                                                                                                                    <  4:195159/148‑1 (MQ=255)
               gggTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCt                                                                                                                                >  7:212073/1‑149 (MQ=255)
                   aaCGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCgg                                                                                                                            >  6:72974/1‑149 (MQ=255)
                    aCGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCGCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCgg                                                                                                                            <  7:168259/148‑1 (MQ=255)
                       ctGGCCGGCGCGAACTCGGGTGTCAGCCGGCCATTTTCGTCGAGCACCCAGGCGTCCATGATCTCGCGTACCACCGCAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTgg                                                                                                                        <  6:195620/148‑1 (MQ=255)
                        tGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTggg                                                                                                                       <  5:72974/149‑1 (MQ=255)
                          gcgggcgCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTgggg                                                                                                                      <  4:202521/148‑1 (MQ=255)
                                         ggTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAg                                                                                                      <  5:157297/149‑1 (MQ=255)
                                                    gCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTcgc                                                                                           <  8:212073/149‑1 (MQ=255)
                                                        ttttCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTg                                                                                       <  6:40001/149‑1 (MQ=255)
                                                        ttttCGTCGAGCAGCCAGGCGTCCATGAGCTGGCGTAGCACCGGAGCGGCGACACCCGTGCCGGTCTCACAGTTCTCGACCTTCACCGATACCACGATTTTCGGGGCTTCGGCTGGTGCAAACGCGACGAACAGGGCATGGTCGCGGTg                                                                                       <  6:273930/149‑1 (MQ=255)
                                                               cgAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAATCCACGATTTTCGGGGCTTCGGCTggg                                                                                                                       >  2:318788/1‑110 (MQ=255)
                                                               cgAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAATCCACGATTTTCGGGGCTTCGGCTggg                                                                                                                       >  2:313967/1‑110 (MQ=255)
                                                               cgAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAATCCACGATTTTCGGGGCTTCGGCTggg                                                                                                                       <  1:318788/110‑1 (MQ=255)
                                                               cgAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAATCCACGATTTTCGGGGCTTCGGCTggg                                                                                                                       <  1:313967/110‑1 (MQ=255)
                                                                  gcagcCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTgg                                                                             <  7:256589/149‑1 (MQ=255)
                                                                      ccAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGtt                                                                         >  8:165297/1‑149 (MQ=255)
                                                                        aGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCACACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTttg                                                                       <  1:67629/149‑1 (MQ=255)
                                                                        aGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTttg                                                                       <  1:306035/149‑1 (MQ=255)
                                                                                aTGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCa                                                                                                  <  7:42823/114‑1 (MQ=255)
                                                                                aTGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCa                                                                                                  >  8:42823/1‑114 (MQ=255)
                                                                                aTGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATc                                                               >  5:153281/1‑149 (MQ=255)
                                                                                       ggCGTACCACCGGAGCGGCGACACCCGAGCCCGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTtc                                                        >  3:261878/1‑149 (MQ=255)
                                                                                           tACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTcgcg                                                                                          <  8:250947/111‑1 (MQ=255)
                                                                                           tACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTcgcg                                                                                          >  7:250947/1‑111 (MQ=255)
                                                                                               accGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTg                                                >  5:253899/1‑149 (MQ=255)
                                                                                                ccGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGt                                                             >  8:240769/1‑135 (MQ=255)
                                                                                                ccGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGt                                                             <  7:240769/135‑1 (MQ=255)
                                                                                                   gAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTg                                            <  7:165297/149‑1 (MQ=255)
                                                                                                               ccGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCaccacc                                >  6:306249/1‑149 (MQ=255)
                                                                                                                 gAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTg                              <  6:253899/149‑1 (MQ=255)
                                                                                                                       gACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCgg                         >  3:274296/1‑148 (MQ=255)
                                                                                                                          tCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTAcc                     >  5:242526/1‑149 (MQ=255)
                                                                                                                          tCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTAcc                     >  7:258870/1‑149 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGc                                                                   >  1:72968/1‑101 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGc                                                                   <  2:72968/101‑1 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATc                                         <  5:317887/127‑1 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATc                                         >  6:317887/1‑127 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCg                    <  8:258870/148‑1 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGc                   >  4:260740/1‑149 (MQ=255)
                                                                                                                            aCCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGc                   >  3:142619/1‑149 (MQ=255)
                                                                                                                              ctttCTCGACCATCACCCAAACCACGATTTTCGGGGCTTCGCCTGGGGCAAACGAGACGAACAGGGCATAGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATAGTACTTCTCGCCCTGCTAGATCGCCACCACCTGCGCGGTACCGctc                 >  2:79037/3‑149 (MQ=37)
                                                                                                                                     gACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTTGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCg           <  6:153281/148‑1 (MQ=255)
                                                                                                                                     gACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGAGGTACCGCTCTTTCCgg          >  4:2966/1‑149 (MQ=255)
                                                                                                                                       ccATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGc         >  4:132566/1‑148 (MQ=255)
                                                                                                                                             ccGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCgg  <  4:274296/149‑1 (MQ=255)
                                                                                                                                             ccGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCgg  >  7:65058/1‑149 (MQ=255)
                                                                                                                                              cGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGc                           >  3:74575/1‑123 (MQ=255)
                                                                                                                                              cGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGc                           <  4:74575/123‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACAGGGCCGATTCCTGGGTAACGGTGGCGGGCGCGAACTCGGGTTTGAGCCGGCCATTTTCGTCGAGCAGCCAGGCGTCCATGATCTGGCGTACCACCGGAGCGGCGACACCCGAGCCGGACTCACCGTTCTCGACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGGCAATGCGG  >  NC_002947/5470017‑5470306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑