Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,472,156 T→A 8.9% K95M (AAG→ATG) ‡ rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,472,1560TA 8.9% 184.4 / 5.0 64K95M (AAG→ATG) ‡rlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base A (3/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGCGGCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGG  >  NC_002947/5472009‑5472303
                                                                                                                                                   |                                                                                                                                                   
gTCAGCGGCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCtt                                                                                                                                                    <  3:107260/149‑1 (MQ=255)
   aGCGGCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGcc                                                                                                                                                 <  2:174663/149‑1 (MQ=255)
   aGCGGCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGc                                                                                                                                                  >  5:22926/1‑148 (MQ=255)
       gcgACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGGCATg                                                                                                                                              >  7:87520/1‑148 (MQ=255)
        cgACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATGGa                                                                                                                                            <  8:119736/149‑1 (MQ=255)
        cgACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCCCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATGGa                                                                                                                                            <  6:260525/149‑1 (MQ=255)
           cAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCt                                                                                                                                         <  5:193067/149‑1 (MQ=255)
             gCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCg                                                                                                                                       <  1:80990/149‑1 (MQ=255)
             gCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCg                                                                                                                                       <  7:190353/149‑1 (MQ=255)
              cGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTc                                                                                                                                        >  4:25918/1‑147 (MQ=255)
              cGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGa                                                                                                                                      <  1:154911/149‑1 (MQ=255)
              cGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGa                                                                                                                                      <  7:95846/149‑1 (MQ=255)
               gACCAACGTTGCTCGGCGCGCGCGCAAACCTCCGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAg                                                                                                                                     <  4:220567/149‑1 (MQ=255)
               gACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAg                                                                                                                                     <  3:25918/149‑1 (MQ=255)
                    aCGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAg                                                                                                                                     <  6:161884/144‑1 (MQ=255)
                    aCGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAg                                                                                                                                     >  5:161884/1‑144 (MQ=255)
                                gcgcgcgcAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAggg                                                                                                                                   <  6:93747/134‑1 (MQ=255)
                                gcgcgcgcAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAggg                                                                                                                                   >  5:93747/1‑134 (MQ=255)
                                     cgcAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGc                                                                                                               <  2:138827/149‑1 (MQ=255)
                                             tCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATGGagtacc                                                                                                                                       <  2:59231/117‑6 (MQ=255)
                                             tCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCATGGagtacc                                                                                                                                       >  1:59231/1‑112 (MQ=255)
                                              cAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAgg                                                                                                                >  2:64483/1‑139 (MQ=255)
                                              cAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAgg                                                                                                                <  1:64483/139‑1 (MQ=255)
                                               aGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGc                                                                                                     >  3:63342/1‑149 (MQ=255)
                                                 gcgcCTGGCCCTCCTGGCCGCCCACCTTGAATTTCACCGTGCGCGCATCCAGGCGCCAGCTGTCCAGCTCGGTCGCCAGCTGCTCGGTACTGCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTg                                                                                                             <  1:264221/139‑1 (MQ=255)
                                                 gcgcCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCCACAGCTGCTCGGTACTCCATGGCTTGCCAAGGACCTCGACGGTGACAATGCGTTCCCCAGGCTg                                                                                                             >  2:264221/1‑139 (MQ=255)
                                                   gcCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAg                                                                                                 <  8:87520/149‑1 (MQ=255)
                                                         cccTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGc                                                                                           >  3:114316/1‑149 (MQ=255)
                                                              cGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTc                                                                                        <  2:262132/147‑1 (MQ=255)
                                                              cGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCg                                                                                       <  6:22926/148‑1 (MQ=255)
                                                               gggCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGcc                                                                                     >  7:1806/1‑149 (MQ=255)
                                                                      ccaccaTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCAAGGACCTCGAGGCTGACAAAGCGTTCCCCAGGAAGCACTATGCTCACCATGCCTTCGCCCGCCTg                                                                               >  8:262442/1‑148 (MQ=255)
                                                                          caTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAAGGTGACAATGCGTTcccc                                                                                                                   >  4:39105/1‑108 (MQ=255)
                                                                          caTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAAGGTGACAATGCGTTcccc                                                                                                                   <  3:39105/108‑1 (MQ=255)
                                                                          caTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCCGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCac                                                                                                                                               >  4:221120/1‑79 (MQ=255)
                                                                           aTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGCCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCGCCTGatccgt                                                                         >  3:97218/1‑144 (MQ=255)
                                                                            tCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGCCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCGCCTGatccgtc                                                                        <  4:97218/149‑7 (MQ=255)
                                                                                aTTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggc                                                                    >  6:222174/1‑149 (MQ=255)
                                                                                aTTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggc                                                                    >  5:209443/1‑149 (MQ=255)
                                                                                   cACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggcgg                                                                  <  6:73061/148‑1 (MQ=255)
                                                                                    aCCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggcgggc                                                                <  5:222174/149‑1 (MQ=255)
                                                                                       gTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTTCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTGTGCTCAGCATGGCTTCGCCCGCCTGATCCGTCAGGAGGGCGaa                                                             <  7:262442/149‑2 (MQ=255)
                                                                                             gcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACg                                                                            >  7:101310/1‑128 (MQ=255)
                                                                                             gcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACg                                                                            <  8:101310/128‑1 (MQ=255)
                                                                                             gcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGAAATCGAGGGTTACAATGCGTTCCCCAGTCTGCACTTTGCTCAGCATGGCTTCGCCCGCCTGATCCGTCAGGAGGGCGaagccatg                                                       <  4:112433/149‑8 (MQ=37)
                                                                                             gcATCCAGGCGCCAGCGGTACAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCgg                                                        >  1:31341/1‑148 (MQ=255)
                                                                                                  cAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCt                                                  >  3:305129/1‑149 (MQ=255)
                                                                                                  cAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCt                                                  <  4:114316/149‑1 (MQ=255)
                                                                                                   cgccgcCAGCGGTCCAGCTCGGTCGCCCGCTGCTCGGTACTCCAGGGCTGGCCCTGGACCTCGAGGGTGACAATGCGGTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                                 <  4:251571/146‑1 (MQ=255)
                                                                                                   aGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                                 >  2:109163/1‑149 (MQ=255)
                                                                                                   aGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                                 >  4:179177/1‑149 (MQ=255)
                                                                                                       gcCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCa                                             <  4:305129/149‑1 (MQ=255)
                                                                                                        ccAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCAc                                            <  6:209443/149‑1 (MQ=255)
                                                                                                                     tCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCgg                               >  8:181863/1‑149 (MQ=255)
                                                                                                                      cGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCgg                               >  3:164497/1‑148 (MQ=255)
                                                                                                                       ggTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCggg                              <  4:164497/148‑1 (MQ=255)
                                                                                                                             cAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTcca                       >  6:112263/1‑149 (MQ=255)
                                                                                                                             cAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTcca                       >  3:45769/1‑149 (MQ=255)
                                                                                                                              aGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTccac                      <  4:63342/149‑1 (MQ=255)
                                                                                                                                ctgctCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTccac                      >  8:190450/1‑147 (MQ=255)
                                                                                                                                         ggtCCATGGCAAGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGc            >  2:2994/3‑148 (MQ=255)
                                                                                                                                          cTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGa          >  7:163079/1‑149 (MQ=255)
                                                                                                                                            ccATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGa          >  8:278195/1‑147 (MQ=255)
                                                                                                                                             cATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAg       >  1:19227/1‑149 (MQ=255)
                                                                                                                                                  cTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGTTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATCCAGCGGGATTTCCACCAGCTCCAGCGACAGCTcgg  >  3:62574/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCAGCGGCGACAGCGACCAACGTTGCTCGGCGCGCGCGCAAACCTCAGGCGCCAGGCCCTCCGGGCCGCCCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGG  >  NC_002947/5472009‑5472303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑