Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,472,222 T→G 7.7% E73A (GAG→GCG)  rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,472,2220TG 7.7% 188.4 / 4.3 62E73A (GAG→GCG) rlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base T (33/24);  new base G (2/3);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTCGGCATGCGCGAGCCGAC  >  NC_002947/5472079‑5472367
                                                                                                                                               |                                                                                                                                                 
ccaccaTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCAAGCCAAGGACCTCGAGGCTGACAAAGCGTTCCCCAGGAAGCACTATGCTCACCATGCCTTCGCCCGCCTg                                                                                                                                               >  8:262442/1‑148 (MQ=255)
     aTCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGCCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCGCCTGatccgt                                                                                                                                         >  3:97218/1‑144 (MQ=255)
      tCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGCCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCGCCTGatccgtc                                                                                                                                        <  4:97218/149‑7 (MQ=255)
          aTTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggc                                                                                                                                    >  5:209443/1‑149 (MQ=255)
          aTTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggc                                                                                                                                    >  6:222174/1‑149 (MQ=255)
             cACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggcgg                                                                                                                                  <  6:73061/148‑1 (MQ=255)
              aCCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAggcgggc                                                                                                                                <  5:222174/149‑1 (MQ=255)
                 gTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTTCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTGTGCTCAGCATGGCTTCGCCCGCCTGATCCGTCAGGAGGGCGaa                                                                                                                             <  7:262442/149‑2 (MQ=255)
                       gcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACg                                                                                                                                            <  8:101310/128‑1 (MQ=255)
                       gcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACg                                                                                                                                            >  7:101310/1‑128 (MQ=255)
                       gcATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGAAATCGAGGGTTACAATGCGTTCCCCAGTCTGCACTTTGCTCAGCATGGCTTCGCCCGCCTGATCCGTCAGGAGGGCGaagccatg                                                                                                                       <  4:112433/149‑8 (MQ=37)
                       gcATCCAGGCGCCAGCGGTACAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCgg                                                                                                                        >  1:31341/1‑148 (MQ=255)
                            cAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCt                                                                                                                  <  4:114316/149‑1 (MQ=255)
                            cAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCt                                                                                                                  >  3:305129/1‑149 (MQ=255)
                             cgccgcCAGCGGTCCAGCTCGGTCGCCCGCTGCTCGGTACTCCAGGGCTGGCCCTGGACCTCGAGGGTGACAATGCGGTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                                                                                                 <  4:251571/146‑1 (MQ=255)
                             aGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                                                                                                 >  2:109163/1‑149 (MQ=255)
                             aGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCtt                                                                                                                 >  4:179177/1‑149 (MQ=255)
                                 gcCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCa                                                                                                             <  4:305129/149‑1 (MQ=255)
                                  ccAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCAc                                                                                                            <  6:209443/149‑1 (MQ=255)
                                               tCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCgg                                                                                               >  8:181863/1‑149 (MQ=255)
                                                cGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCgg                                                                                               >  3:164497/1‑148 (MQ=255)
                                                 ggTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCggg                                                                                              <  4:164497/148‑1 (MQ=255)
                                                       cAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTcca                                                                                       >  3:45769/1‑149 (MQ=255)
                                                       cAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTcca                                                                                       >  6:112263/1‑149 (MQ=255)
                                                        aGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTccac                                                                                      <  4:63342/149‑1 (MQ=255)
                                                          ctgctCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTccac                                                                                      >  8:190450/1‑147 (MQ=255)
                                                                   ggtCCATGGCAAGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGc                                                                            >  2:2994/3‑148 (MQ=255)
                                                                    cTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGa                                                                          >  7:163079/1‑149 (MQ=255)
                                                                      ccATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGa                                                                          >  8:278195/1‑147 (MQ=255)
                                                                       cATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAg                                                                       >  1:19227/1‑149 (MQ=255)
                                                                            cTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGTTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATCCAGCGGGATTTCCACCAGCTCCAGCGACAGCTcgg                                                                  >  3:62574/1‑149 (MQ=255)
                                                                               gCCATGGACCTCGAGGGTGGCAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTcggcgg                                                               >  8:127737/1‑149 (MQ=255)
                                                                                ccATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCggg                                                              >  4:56697/1‑149 (MQ=255)
                                                                                 cATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCTCTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCggg                                                              >  6:95815/1‑148 (MQ=255)
                                                                                 cATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCggg                                                              >  2:217810/1‑148 (MQ=255)
                                                                                   tGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAg                                                           >  4:104628/1‑149 (MQ=255)
                                                                                    ggACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCggg                                                              <  4:213567/145‑1 (MQ=255)
                                                                                    ggACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCggg                                                              >  3:213567/1‑145 (MQ=255)
                                                                                       ccTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGt                                                                                                        <  5:216416/100‑1 (MQ=255)
                                                                                       ccTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGt                                                                                                        >  6:216416/1‑100 (MQ=255)
                                                                                           gAGGGTGGCAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTg                                                    <  7:127737/148‑1 (MQ=255)
                                                                                           gAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCtt                                                     <  7:278195/147‑1 (MQ=255)
                                                                                           gAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTgg                                                   <  7:190450/149‑1 (MQ=255)
                                                                                           gAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTg                                                    <  1:109163/148‑1 (MQ=255)
                                                                                           gAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTg                                                    <  2:31341/148‑1 (MQ=255)
                                                                                               gTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCg                                                                                                <  3:131153/100‑1 (MQ=255)
                                                                                               gTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCg                                                                                                >  4:131153/1‑100 (MQ=255)
                                                                                                tGACAATGCGTTCCCCAGGCTGCTCTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCatat                                              <  5:95815/149‑1 (MQ=255)
                                                                                                   cAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCa                                           >  7:293332/1‑149 (MQ=255)
                                                                                                    aaTGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCAt                                          >  4:270540/1‑149 (MQ=255)
                                                                                                             cccAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCa                                      <  2:242902/144‑1 (MQ=255)
                                                                                                             cccAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCa                                      >  1:242902/1‑144 (MQ=255)
                                                                                                              ccAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTc                                >  5:231118/1‑149 (MQ=255)
                                                                                                               cAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTcc                               >  2:290737/1‑149 (MQ=255)
                                                                                                                      cACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGAccc                        <  7:181863/149‑1 (MQ=255)
                                                                                                                            gCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTc                   <  3:104628/148‑1 (MQ=255)
                                                                                                                             cTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTCg                  <  2:19227/148‑1 (MQ=255)
                                                                                                                              tCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTCgg                 >  8:108748/1‑148 (MQ=255)
                                                                                                                                 gCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGAccc                        >  6:111361/1‑138 (MQ=255)
                                                                                                                                 gCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGAccc                        <  5:111361/138‑1 (MQ=255)
                                                                                                                                    tGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTCGGCATgcgc          <  1:217810/149‑1 (MQ=255)
                                                                                                                                            cccTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTCGGCATGCGCGAGCCGAc  >  5:292208/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CCACCATCAAATTCACCGTGCGCGCATCCAGGCGCCAGCGGTCCAGCTCGGTCGCCAGCTGCTCGGTACTCCATGGCTTGCCATGGACCTCGAGGGTGACAATGCGTTCCCCAGGCTGCACTTTGCTCAGCATGGCTTCGCCCTCCTGACGGATCAGGCGGGCGACGTCGGCATTCTTGCCACGGGTATTCAGCGGGATTTCCACCAGCTCCAGCGACAGCTCGGCGGGCAGGCGCTTGGCATATTCATGCCAGCCTTCCTCGACCCACTTCGGCATGCGCGAGCCGAC  >  NC_002947/5472079‑5472367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑