| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,552,913 | T→G | 11.4% | intergenic (+576/+85) | PP_4882 → / ← PP_5717 | iron ABC transporter permease/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,552,913 | 0 | T | G | 11.4% | 125.5 / 6.7 | 51 | intergenic (+576/+85) | PP_4882/PP_5717 | iron ABC transporter permease/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (19/26); new base G (4/2); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
CGGTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTA > NC_002947/5552766‑5553059 | cGGTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCt < 6:19191/148‑1 (MQ=255) tACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCg > 5:136186/1‑148 (MQ=255) ttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGccc < 8:168592/148‑1 (MQ=255) ttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGccc < 1:320113/148‑1 (MQ=255) tCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAGCCCg < 8:250395/148‑1 (MQ=255) gTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCGTTTGGTAGCAAAGCCCCGCAACAGGGGgtt > 4:148830/1‑147 (MQ=17) cACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCGGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAg < 4:224793/148‑1 (MQ=255) tGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg > 7:193161/1‑149 (MQ=255) cgcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg < 7:299276/147‑1 (MQ=255) gcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg < 8:9272/149‑1 (MQ=255) gcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg < 6:136186/149‑1 (MQ=255) aTCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGcc < 3:173412/122‑1 (MQ=255) tCAGTTGGTACGTCTGGCGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg < 4:260367/132‑1 (MQ=255) tCAGTTGGTACGTCTGGCGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg > 3:260367/1‑132 (MQ=255) gTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg < 8:50364/129‑1 (MQ=255) gTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg > 7:50364/1‑129 (MQ=255) gTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCtt > 1:210353/1‑149 (MQ=255) ttcttGGTGAACCGGCTGTACACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGGGTTGGTTAGCAAATCCCGGGAAAAGGGGTTTGAGCTGGTGGGTGCGTGTGtttt > 7:114609/5‑149 (MQ=9) cGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGtt < 3:148830/149‑1 (MQ=255) tCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTGTTAGCCAAGCCCCGCAAAAGGGGGTTGAGCGGGTGGGTGGTTGAGTGtttt > 3:301306/1‑148 (MQ=17) tACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAAc < 5:191663/148‑1 (MQ=255) gTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTAGCAAAGCCCGGAAACTGGGGTTTAAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTg > 8:164789/1‑149 (MQ=255) aCCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTAGTAGCAAAGCCCGGCAACTGGCGTTTGAGCTGGTGGGTGGTTGAGTGGGTATAAAACAAATGGtttgaa > 3:137993/1‑144 (MQ=14) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg < 6:91073/113‑1 (MQ=255) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg > 5:91073/1‑113 (MQ=255) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg < 4:258520/110‑1 (MQ=255) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg > 3:258520/1‑110 (MQ=255) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAGccc > 6:269070/1‑89 (MQ=14) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAGccc < 5:269070/89‑1 (MQ=14) cAAGAAGCAGGCTTATGCTGAACAAGGTCTAGAGGAAATCCGAACCAGAAACCAATACAGGCAGGTCCGGGCTATGCTTCGCAAAGCCCGGAAAATGGGGTTTCAGGTGGTGGCTGGTTGAGTGTTTAGAAAACAATGCGTTGCATTCt > 1:241402/1‑149 (MQ=255) aagaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGGTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAAt > 5:108613/1‑136 (MQ=255) aagaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAAt < 6:108613/136‑1 (MQ=255) gaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGtt < 2:210353/149‑1 (MQ=255) aagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGttt < 2:258165/149‑1 (MQ=255) ttATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTGGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATTGGTTGGATTCTGGTTGTCCAggg > 5:107889/1‑149 (MQ=17) tGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGGTTAGCAAAGCCCGGGATCTGGGGTTTGAGCTGGTGGCTGCTTGAGTGTTTTGAAAACAATTGGGTGCATTCTTTTTGACCAgggggt > 1:204317/1‑146 (MQ=17) gCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg < 7:129060/97‑1 (MQ=255) gCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTa > 3:92362/1‑149 (MQ=255) cAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTc > 1:22079/1‑147 (MQ=255) aaGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGc < 1:222747/84‑1 (MQ=255) aaGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAg < 3:13328/149‑1 (MQ=255) tCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTAct < 7:164789/149‑1 (MQ=255) aGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATc > 5:255129/1‑149 (MQ=255) ggAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATcc < 8:114609/149‑1 (MQ=255) ttCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAg < 4:154184/149‑1 (MQ=255) cGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGaaa < 4:301306/149‑1 (MQ=255) aaGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGaa < 6:255129/149‑1 (MQ=255) aCCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGt > 7:229953/1‑149 (MQ=255) cGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGc < 4:92362/149‑1 (MQ=255) tCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAg > 3:314213/1‑148 (MQ=255) gggCTTTGCGAAGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCaa > 7:110686/1‑148 (MQ=255) cTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTa < 8:193161/148‑1 (MQ=255) | CGGTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTA > NC_002947/5552766‑5553059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |