Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 448,800 C→T 7.1% intergenic (+95/‑30) PP_0369 → / → PP_0370 diguanylate cyclase/acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947448,8000CT 7.1% 166.0 / 2.5 56intergenic (+95/‑30)PP_0369/PP_0370diguanylate cyclase/acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (29/23);  new base T (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CATGGGCGACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGA  >  NC_002947/448664‑448942
                                                                                                                                        |                                                                                                                                              
cATGGGCGACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCcccgaccc                                                                                                                                     <  8:91609/148‑1 (MQ=255)
cATGGGCGACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCcccgaccc                                                                                                                                     >  6:82322/1‑148 (MQ=255)
     gCGACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAg                                                                                                                               >  2:310303/1‑149 (MQ=255)
     gCGACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAg                                                                                                                               >  4:131166/1‑149 (MQ=255)
       gACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGc                                                                                                                              >  3:286963/1‑148 (MQ=255)
          aaTCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCga                                                                                                                            >  1:237779/1‑147 (MQ=255)
                cgcgTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGt                                                                                                                    >  6:2941/1‑149 (MQ=255)
                     ggggCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATgg                                                                                                               <  4:286963/149‑1 (MQ=255)
                     ggggCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATgg                                                                                                               >  2:104430/1‑149 (MQ=255)
                         cAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGa                                                                                                           >  3:191857/1‑149 (MQ=255)
                         cAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACCGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGa                                                                                                           >  5:172689/1‑149 (MQ=255)
                                            gAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACCGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGATTATAAAGCGCCGCTgcgc                                                                                        <  6:172689/149‑1 (MQ=255)
                                                 agaCTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACAt                                                                                   >  3:149535/1‑149 (MQ=255)
                                                  gaCTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATg                                                                                  <  5:244901/149‑1 (MQ=255)
                                                  gaCTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAAACCCAGTGTCGAGGTTCCACGAGACAGCCCCGACCCGACTAGCGACAGGTTGTCATGGCTGACTATAAAGCGCAGCTGCGCGACATg                                                                                  >  7:127959/1‑149 (MQ=255)
                                                    cTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATgc                                                                                 <  3:131166/148‑1 (MQ=255)
                                                         cAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGa                                                                                      <  2:75176/138‑1 (MQ=255)
                                                         cAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGa                                                                                      >  1:75176/1‑138 (MQ=255)
                                                         cAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCACCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCg                                                                           <  5:231543/149‑1 (MQ=255)
                                                           gTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGa                                                                                                           >  6:309064/1‑115 (MQ=255)
                                                           gTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGa                                                                                                           <  5:309064/115‑1 (MQ=255)
                                                            tCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTAc                                                                        >  5:111187/1‑149 (MQ=255)
                                                              gATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGagag                                                                                                                         >  1:176085/1‑98 (MQ=255)
                                                              gATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGagag                                                                                                                         <  2:176085/98‑1 (MQ=255)
                                                                  gatgaCCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACtgaat                                                                   <  5:82322/148‑1 (MQ=255)
                                                                    tgccccTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACtgaatgaa                                                                <  6:251531/146‑1 (MQ=255)
                                                                    tgaCCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACtgaatgaa                                                                <  3:255738/149‑1 (MQ=255)
                                                                     gaCCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGATCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAg                                                               >  8:46026/1‑149 (MQ=255)
                                                                     gaCCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGATCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAg                                                               >  8:41370/1‑149 (MQ=255)
                                                                        ccTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCt                                                            >  8:75375/1‑149 (MQ=255)
                                                                                  gCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGc                                                  <  4:149535/149‑1 (MQ=255)
                                                                                  gCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGc                                                  >  8:126747/1‑149 (MQ=255)
                                                                                  gCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGc                                                  >  7:103519/1‑149 (MQ=255)
                                                                                  gCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGc                                                  >  6:70927/1‑149 (MQ=255)
                                                                                   taaaaGTTGTTCGGTTACACTGCCAGTAACCCGAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGcc                                                 <  8:127959/148‑1 (MQ=255)
                                                                                    aaaaGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGcc                                                 >  2:201457/1‑148 (MQ=255)
                                                                                            ttCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTg                                         <  4:191857/148‑1 (MQ=255)
                                                                                                   acacTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGa                                               >  1:106919/1‑135 (MQ=255)
                                                                                                   acacTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGa                                               <  2:106919/135‑1 (MQ=255)
                                                                                                   acacTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAg                                  <  1:201457/148‑1 (MQ=255)
                                                                                                       tGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGcc                             >  7:95680/1‑149 (MQ=255)
                                                                                                         ccAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGccc                            <  2:237779/148‑1 (MQ=255)
                                                                                                          cAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCgg                          <  8:103519/149‑1 (MQ=255)
                                                                                                           aGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCggg                         >  4:182046/1‑149 (MQ=255)
                                                                                                               aCCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTgg                     <  8:95680/149‑1 (MQ=255)
                                                                                                                 cccAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGcc                   >  7:243356/1‑149 (MQ=255)
                                                                                                                  ccAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCg                  >  7:169945/1‑149 (MQ=255)
                                                                                                                    aGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAg                <  7:126747/149‑1 (MQ=255)
                                                                                                                     gtgtCGCGGTTCCCCGAGATCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAgg               <  7:46026/149‑1 (MQ=255)
                                                                                                                     gtgtCGCGGTTCCCCGAGATCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAgg               <  7:41370/149‑1 (MQ=255)
                                                                                                                      tgtCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGt              >  5:320564/1‑149 (MQ=255)
                                                                                                                           cgGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCg         <  3:182046/149‑1 (MQ=255)
                                                                                                                             gTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTgg                     >  3:313016/1‑135 (MQ=255)
                                                                                                                             gTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTgg                     <  4:313016/135‑1 (MQ=255)
                                                                                                                                 cccGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGcc    >  4:231628/1‑148 (MQ=255)
                                                                                                                                  ccGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGa  >  1:91938/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                              
CATGGGCGACAATCACCGCGTGGGGCAGTGCGTACGGCGTGACCGAATGAGACTATGCAGTCGATTGATGACCCTATGTCTCGCAAAAGTTGTTCGGTTACACTGCCAGTAACCCCAGTGTCGCGGTTCCCCGAGACCGCCCCGACCCGACTAGCGAGAGGTTGTCATGGCTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGA  >  NC_002947/448664‑448942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑