Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,552,915 C→A 17.4% intergenic (+578/+83) PP_4882 → / ← PP_5717 iron ABC transporter permease/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,552,9150CA17.4% 115.7 / 5.1 51intergenic (+578/+83)PP_4882/PP_5717iron ABC transporter permease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (14/26);  new base A (8/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTA  >  NC_002947/5552769‑5553059
                                                                                                                                                  |                                                                                                                                                
tACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCg                                                                                                                                                 >  5:136186/1‑148 (MQ=255)
        ttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGccc                                                                                                                                         <  8:168592/148‑1 (MQ=255)
        ttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGccc                                                                                                                                         <  1:320113/148‑1 (MQ=255)
         tCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAGCCCg                                                                                                                                        <  8:250395/148‑1 (MQ=255)
                       gTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCGTTTGGTAGCAAAGCCCCGCAACAGGGGgtt                                                                                                                           >  4:148830/1‑147 (MQ=17)
                         cACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCGGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAg                                                                                                                        <  4:224793/148‑1 (MQ=255)
                            tGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg                                                                                                                    >  7:193161/1‑149 (MQ=255)
                              cgcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg                                                                                                                    <  7:299276/147‑1 (MQ=255)
                               gcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg                                                                                                                 <  8:9272/149‑1 (MQ=255)
                               gcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg                                                                                                                 <  6:136186/149‑1 (MQ=255)
                                 aTCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGcc                                                                                                                                          <  3:173412/122‑1 (MQ=255)
                                  tCAGTTGGTACGTCTGGCGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg                                                                                                                               <  4:260367/132‑1 (MQ=255)
                                  tCAGTTGGTACGTCTGGCGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg                                                                                                                               >  3:260367/1‑132 (MQ=255)
                                     gTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg                                                                                                                               <  8:50364/129‑1 (MQ=255)
                                     gTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTggg                                                                                                                               >  7:50364/1‑129 (MQ=255)
                                     gTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCtt                                                                                                           >  1:210353/1‑149 (MQ=255)
                                            ttcttGGTGAACCGGCTGTACACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGGGTTGGTTAGCAAATCCCGGGAAAAGGGGTTTGAGCTGGTGGGTGCGTGTGtttt                                                                                                    >  7:114609/5‑149 (MQ=9)
                                            cGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGtt                                                                                                    <  3:148830/149‑1 (MQ=255)
                                              tCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTGTTAGCCAAGCCCCGCAAAAGGGGGTTGAGCGGGTGGGTGGTTGAGTGtttt                                                                                                  >  3:301306/1‑148 (MQ=17)
                                                     tACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAAc                                                                                            <  5:191663/148‑1 (MQ=255)
                                                             gTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTAGCAAAGCCCGGAAACTGGGGTTTAAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTg                                                                                   >  8:164789/1‑149 (MQ=255)
                                                                 aCCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTAGTAGCAAAGCCCGGCAACTGGCGTTTGAGCTGGTGGGTGGTTGAGTGGGTATAAAACAAATGGtttgaa                                                                                >  3:137993/1‑144 (MQ=14)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg                                                                                                                 <  6:91073/113‑1 (MQ=255)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg                                                                                                                 >  5:91073/1‑113 (MQ=255)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg                                                                                                                    <  4:258520/110‑1 (MQ=255)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtgg                                                                                                                    >  3:258520/1‑110 (MQ=255)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAGccc                                                                                                                                         >  6:269070/1‑89 (MQ=14)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAGccc                                                                                                                                         <  5:269070/89‑1 (MQ=14)
                                                                   cAAGAAGCAGGCTTATGCTGAACAAGGTCTAGAGGAAATCCGAACCAGAAACCAATACAGGCAGGTCCGGGCTATGCTTCGCAAAGCCCGGAAAATGGGGTTTCAGGTGGTGGCTGGTTGAGTGTTTAGAAAACAATGCGTTGCATTCt                                                                             >  1:241402/1‑149 (MQ=255)
                                                                    aagaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGGTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAAt                                                                                         >  5:108613/1‑136 (MQ=255)
                                                                    aagaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAAt                                                                                         <  6:108613/136‑1 (MQ=255)
                                                                      gaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGtt                                                                          <  2:210353/149‑1 (MQ=255)
                                                                       aagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGttt                                                                         <  2:258165/149‑1 (MQ=255)
                                                                               ttATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTGGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATTGGTTGGATTCTGGTTGTCCAggg                                                                 >  5:107889/1‑149 (MQ=17)
                                                                                  tGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGGTTAGCAAAGCCCGGGATCTGGGGTTTGAGCTGGTGGCTGCTTGAGTGTTTTGAAAACAATTGGGTGCATTCTTTTTGACCAgggggt                                                              >  1:204317/1‑146 (MQ=17)
                                                                                   gCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCtggtgg                                                                                                                 <  7:129060/97‑1 (MQ=255)
                                                                                   gCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTa                                                             >  3:92362/1‑149 (MQ=255)
                                                                                         cAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTc                                                         >  1:22079/1‑147 (MQ=255)
                                                                                          aaGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGc                                                                                                                       <  1:222747/84‑1 (MQ=255)
                                                                                          aaGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAg                                                      <  3:13328/149‑1 (MQ=255)
                                                                                              tCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTAct                                                  <  7:164789/149‑1 (MQ=255)
                                                                                                   aGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATc                                             >  5:255129/1‑149 (MQ=255)
                                                                                                    ggAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATcc                                            <  8:114609/149‑1 (MQ=255)
                                                                                                        ttCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAg                                        <  4:154184/149‑1 (MQ=255)
                                                                                                           cGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGaaa                                     <  4:301306/149‑1 (MQ=255)
                                                                                                                   aaGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGaa                             <  6:255129/149‑1 (MQ=255)
                                                                                                                           aCCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGt                     >  7:229953/1‑149 (MQ=255)
                                                                                                                             cGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGc                   <  4:92362/149‑1 (MQ=255)
                                                                                                                                    tCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAg             >  3:314213/1‑148 (MQ=255)
                                                                                                                                       gggCTTTGCGAAGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCaa          >  7:110686/1‑148 (MQ=255)
                                                                                                                                               cTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTa  <  8:193161/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTA  >  NC_002947/5552769‑5553059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑