Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,561,233	A→T	6.1%	A145A (GCT→GCA)	hflC ←	integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,561,233	0	A	T	6.1%	200.3 / 2.3	65	A145A (GCT→GCA)	hflC	integral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (33/28);  new base T (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCGGGCTTCACGCTCACGCTCGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGT  >  NC_002947/5561086‑5561381                                                                                                                                                    |                                                                                                                                                      gcgGGCTTCACGCTCACGCTCGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAg                                                                                                                                                      >  5:260118/1‑149 (MQ=255)      cTTCACGCTCACGCTCGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCAt                                                                                                                                                  >  6:16125/1‑148 (MQ=255)            gctcacgctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCa                                                                                                                                           >  5:329021/1‑149 (MQ=255)               cacgctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGt                                                                                                                                              <  4:53330/143‑1 (MQ=255)               cacgctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGt                                                                                                                                              >  3:53330/1‑143 (MQ=255)                   ctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGtt                                                                                                                                    <  5:16125/149‑1 (MQ=255)                             aTGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGaa                                                                                                                            <  4:46998/147‑1 (MQ=255)                             aTGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGaa                                                                                                                            <  6:329021/147‑1 (MQ=255)                              tGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAAc                                                                                                                          >  2:76326/1‑148 (MQ=255)                              tGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGCTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTACTTGCTCGCCATGCTATTCAGAGATGCACTGATGGCTGACATCACTGCATc                                                                                                                                         >  1:307815/1‑133 (MQ=255)                              tGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGAATGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCTATGCACTGATGGCTGACATCACTGCATc                                                                                                                                         <  2:307815/133‑1 (MQ=255)                                cGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTg                                                                                                                                             <  6:49414/127‑1 (MQ=255)                                cGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTg                                                                                                                                             >  5:49414/1‑127 (MQ=255)                                          cGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAg‑g                                                                                                            <  6:260118/149‑1 (MQ=255)                                            cTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGt                                                                                                          <  8:141120/149‑1 (MQ=255)                                             tGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTa                                                                                                         >  7:229154/1‑149 (MQ=255)                                               cGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACg                                                                                                       >  1:23160/1‑149 (MQ=255)                                                     aCTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAAc                                                                                                                          <  3:92197/125‑1 (MQ=255)                                                     aCTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAAc                                                                                                                          >  4:92197/1‑125 (MQ=255)                                                       ttccttcGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCg                                                                                                >  4:157886/1‑148 (MQ=255)                                                         ccttcGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGa                                                                                               <  2:23160/147‑1 (MQ=255)                                                               gCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTcg                                                                                       >  5:203710/1‑149 (MQ=255)                                                                 aGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTcgcg                                                                                     >  3:99635/1‑149 (MQ=255)                                                                      gATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGc                                                                                >  6:43503/1‑149 (MQ=255)                                                                            cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAg‑g                                                                                                            >  2:191071/1‑115 (MQ=255)                                                                            cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCa                                                                                   <  1:151746/140‑1 (MQ=255)                                                                            cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCa                                                                                   >  2:151746/1‑140 (MQ=255)                                                                            cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACttt                                                                          >  1:185835/1‑149 (MQ=255)                                                                            cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACAACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACttt                                                                          >  4:247001/1‑149 (MQ=255)                                                                              tGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTAc                                                                                                  >  5:305855/1‑123 (MQ=255)                                                                              tGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTAc                                                                                                  <  6:305855/123‑1 (MQ=255)                                                                               gACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTcc                                                                        <  5:43503/148‑1 (MQ=255)                                                                                aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTc                                                                         >  6:278430/1‑146 (MQ=255)                                                                                aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTc                                                                         >  4:66216/1‑146 (MQ=255)                                                                                aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTc                                                                         >  4:198070/1‑146 (MQ=255)                                                                                aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCa                                                                       <  8:140265/148‑1 (MQ=255)                                                                                 cgcgCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAga                                                                     <  3:155287/149‑1 (MQ=255)                                                                                        gTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGAcgcg                                                              <  8:229154/149‑1 (MQ=255)                                                                                          ccagcaccTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGa                                                             >  5:44617/5‑148 (MQ=255)                                                                                           gACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAGAGGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGa                                                             <  1:76326/148‑1 (MQ=255)                                                                                             ccaccTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGa                                                         >  7:206101/1‑149 (MQ=255)                                                                                              caccTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGCCCACTTTCCAGACGACGCGACAGAc                                                        >  4:252413/1‑149 (MQ=255)                                                                                                    gATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCtc                                                    <  4:99635/147‑1 (MQ=255)                                                                                                            gTTCCTTGCTCGCCATGCGATTCAGCGATGCAGGGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATc                                          <  3:223820/149‑1 (MQ=255)                                                                                                               ccTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTg                                        <  6:203710/148‑1 (MQ=255)                                                                                                                cTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCt                                      <  2:185835/149‑1 (MQ=255)                                                                                                                cTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCt                                      <  3:157886/149‑1 (MQ=255)                                                                                                                cTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCt                                      <  3:198070/149‑1 (MQ=255)                                                                                                                       gCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGc                                <  6:44617/148‑1 (MQ=255)                                                                                                                            gCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACg                          >  3:176006/1‑149 (MQ=255)                                                                                                                             cGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGt                         >  1:87312/1‑149 (MQ=255)                                                                                                                               aTTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                     >  7:63691/1‑135 (MQ=255)                                                                                                                               aTTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                     <  8:63691/135‑1 (MQ=255)                                                                                                                               aTTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTgg                       >  8:151467/1‑149 (MQ=255)                                                                                                                                  cAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCg                     <  2:87312/148‑1 (MQ=255)                                                                                                                                   cgtgATGCACTGATGGCTGACATCACTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgt                   <  6:230281/146‑1 (MQ=255)                                                                                                                                   cgtgATGCACTGATGGCTGACATCACTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgt                   >  5:230281/4‑149 (MQ=255)                                                                                                                                   aGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgt                   >  2:231544/1‑149 (MQ=255)                                                                                                                                    gCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgtg                  >  4:297522/1‑149 (MQ=255)                                                                                                                                             tGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                     >  2:69253/1‑121 (MQ=255)                                                                                                                                             tGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                     <  1:69253/121‑1 (MQ=255)                                                                                                                                               aTGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGtt        <  5:278430/148‑1 (MQ=255)                                                                                                                                               aTGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGtt        <  3:66216/148‑1 (MQ=255)                                                                                                                                                tGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGtt        >  3:275182/1‑147 (MQ=255)                                                                                                                                                   cAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCg   >  7:119810/1‑149 (MQ=255)                                                                                                                                                    aGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGt  >  8:211173/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GCGGGCTTCACGCTCACGCTCGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGT  >  NC_002947/5561086‑5561381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑