Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,561,241	G→C	6.1%	L143V (CTG→GTG)	hflC ←	integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,561,241	0	G	C	6.1%	210.7 / 2.7	65	L143V (CTG→GTG)	hflC	integral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base C (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCTCACGCTCGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGTCCTTGACG  >  NC_002947/5561097‑5561389                                                                                                                                                 |                                                                                                                                                      gctcacgctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCa                                                                                                                                                   >  5:329021/1‑149 (MQ=255)    cacgctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGt                                                                                                                                                      <  4:53330/143‑1 (MQ=255)    cacgctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGt                                                                                                                                                      >  3:53330/1‑143 (MQ=255)        ctcGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGtt                                                                                                                                            <  5:16125/149‑1 (MQ=255)                  aTGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGaa                                                                                                                                    <  4:46998/147‑1 (MQ=255)                  aTGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGaa                                                                                                                                    <  6:329021/147‑1 (MQ=255)                   tGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAAc                                                                                                                                  >  2:76326/1‑148 (MQ=255)                   tGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGCTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTACTTGCTCGCCATGCTATTCAGAGATGCACTGATGGCTGACATCACTGCATc                                                                                                                                                 >  1:307815/1‑133 (MQ=255)                   tGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGAATGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCTATGCACTGATGGCTGACATCACTGCATc                                                                                                                                                 <  2:307815/133‑1 (MQ=255)                     cGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTg                                                                                                                                                     <  6:49414/127‑1 (MQ=255)                     cGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTg                                                                                                                                                     >  5:49414/1‑127 (MQ=255)                               cGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAg‑g                                                                                                                    <  6:260118/149‑1 (MQ=255)                                 cTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGt                                                                                                                  <  8:141120/149‑1 (MQ=255)                                  tGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTa                                                                                                                 >  7:229154/1‑149 (MQ=255)                                    cGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACg                                                                                                               >  1:23160/1‑149 (MQ=255)                                          aCTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAAc                                                                                                                                  >  4:92197/1‑125 (MQ=255)                                          aCTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAAc                                                                                                                                  <  3:92197/125‑1 (MQ=255)                                            ttccttcGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCg                                                                                                        >  4:157886/1‑148 (MQ=255)                                              ccttcGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGa                                                                                                       <  2:23160/147‑1 (MQ=255)                                                    gCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTcg                                                                                               >  5:203710/1‑149 (MQ=255)                                                      aGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTcgcg                                                                                             >  3:99635/1‑149 (MQ=255)                                                           gATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGc                                                                                        >  6:43503/1‑149 (MQ=255)                                                                 cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAg‑g                                                                                                                    >  2:191071/1‑115 (MQ=255)                                                                 cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCa                                                                                           <  1:151746/140‑1 (MQ=255)                                                                 cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCa                                                                                           >  2:151746/1‑140 (MQ=255)                                                                 cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACttt                                                                                  >  1:185835/1‑149 (MQ=255)                                                                 cTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACAACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACttt                                                                                  >  4:247001/1‑149 (MQ=255)                                                                   tGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTAc                                                                                                          >  5:305855/1‑123 (MQ=255)                                                                   tGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTAc                                                                                                          <  6:305855/123‑1 (MQ=255)                                                                    gACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTcc                                                                                <  5:43503/148‑1 (MQ=255)                                                                     aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTc                                                                                 >  4:66216/1‑146 (MQ=255)                                                                     aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTc                                                                                 >  6:278430/1‑146 (MQ=255)                                                                     aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTc                                                                                 >  4:198070/1‑146 (MQ=255)                                                                     aCGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCa                                                                               <  8:140265/148‑1 (MQ=255)                                                                      cgcgCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAga                                                                             <  3:155287/149‑1 (MQ=255)                                                                             gTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGAcgcg                                                                      <  8:229154/149‑1 (MQ=255)                                                                               ccagcaccTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGa                                                                     >  5:44617/5‑148 (MQ=255)                                                                                gACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAGAGGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGa                                                                     <  1:76326/148‑1 (MQ=255)                                                                                  ccaccTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGa                                                                 >  7:206101/1‑149 (MQ=255)                                                                                   caccTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGCCCACTTTCCAGACGACGCGACAGAc                                                                >  4:252413/1‑149 (MQ=255)                                                                                         gATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCtc                                                            <  4:99635/147‑1 (MQ=255)                                                                                                 gTTCCTTGCTCGCCATGCGATTCAGCGATGCAGGGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATc                                                  <  3:223820/149‑1 (MQ=255)                                                                                                    ccTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTg                                                <  6:203710/148‑1 (MQ=255)                                                                                                     cTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCt                                              <  2:185835/149‑1 (MQ=255)                                                                                                     cTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCt                                              <  3:157886/149‑1 (MQ=255)                                                                                                     cTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCt                                              <  3:198070/149‑1 (MQ=255)                                                                                                            gCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGc                                        <  6:44617/148‑1 (MQ=255)                                                                                                                 gCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACg                                  >  3:176006/1‑149 (MQ=255)                                                                                                                  cGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGt                                 >  1:87312/1‑149 (MQ=255)                                                                                                                    aTTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                             >  7:63691/1‑135 (MQ=255)                                                                                                                    aTTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                             <  8:63691/135‑1 (MQ=255)                                                                                                                    aTTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTgg                               >  8:151467/1‑149 (MQ=255)                                                                                                                       cAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCg                             <  2:87312/148‑1 (MQ=255)                                                                                                                        cgtgATGCACTGATGGCTGACATCACTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgt                           <  6:230281/146‑1 (MQ=255)                                                                                                                        cgtgATGCACTGATGGCTGACATCACTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgt                           >  5:230281/4‑149 (MQ=255)                                                                                                                        aGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgt                           >  2:231544/1‑149 (MQ=255)                                                                                                                         gCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGgtg                          >  4:297522/1‑149 (MQ=255)                                                                                                                                  tGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                             >  2:69253/1‑121 (MQ=255)                                                                                                                                  tGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCtt                                             <  1:69253/121‑1 (MQ=255)                                                                                                                                    aTGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGtt                <  5:278430/148‑1 (MQ=255)                                                                                                                                    aTGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGtt                <  3:66216/148‑1 (MQ=255)                                                                                                                                     tGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGtt                >  3:275182/1‑147 (MQ=255)                                                                                                                                        cAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCg           >  7:119810/1‑149 (MQ=255)                                                                                                                                         aGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGt          >  8:211173/1‑149 (MQ=255)                                                                                                                                          gCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGTc         >  8:183232/1‑149 (MQ=255)                                                                                                                                                 gTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGTCCTTGAcg  <  7:211173/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      GCTCACGCTCGGTGCTCATGCGGTCGAACACGCTGCGGTTGACTTCCTTCGGCAGGTCGATGGCCTTGACGCGCACGTCGACCACCTCGATGCCCAGTTCCTTGCTCGCCATGCGATTCAGCGATGCAGTGATGTCAGCCATCAGTGCATCACGTTCACCGGAAACCACTTCGTGCAG‑GGTACGTTTACCGAACTGGTCGCGCAGGCCACTTTCCAGACGACGCGACAGACGCTCGTCGGCGATCTGCTTCATGCCGGACGTGGCGGTGTAGAAGCGTTCAGCGTCCTTGACG  >  NC_002947/5561097‑5561389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑