Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 456,864 C→T 6.4% R296H (CGT→CAT)  pqqE ← coenzyme PQQ synthesis protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947456,8640CT 6.4% 182.6 / 2.7 62R296H (CGT→CAT) pqqEcoenzyme PQQ synthesis protein E
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base T (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

GATGATGCCGTGGTCGGTCGACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGTGATGGTGAGGAACAGGTTGCCCCAGC  >  NC_002947/456716‑457011
                                                                                                                                                    |                                                                                                                                                   
gatgatGCCGTGGTCGGTCGACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCAc                                                                                                                                                     >  2:10572/1‑149 (MQ=255)
       ccGTGGTCGGTCGACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCg                                                                                                                                              <  3:267033/149‑1 (MQ=255)
         gTGGTCGGTCGACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGt                                                                                                                                            <  3:229091/149‑1 (MQ=255)
          tGGTCGGTCGACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGtt                                                                                                                                           <  3:258051/149‑1 (MQ=255)
            gtcggtcgACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGa                                                                                                                                         <  1:121339/149‑1 (MQ=255)
            gtcggtcgACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGa                                                                                                                                         <  6:218737/149‑1 (MQ=255)
            ggcggtcgACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGGTGa                                                                                                                                         <  1:116703/147‑1 (MQ=255)
              cggtcgACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGaaa                                                                                                                                       >  6:330627/1‑149 (MQ=255)
               ggtcgACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAAc                                                                                                                                      <  2:49321/149‑1 (MQ=255)
                gtcgACTTGGCGCACACCGGGTAGGCGTAGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCGGCCGAAGTGCTTTTCTTTCTCGTCGCAGGAACGGCAATGTTCGAGCCTACAATCAAAGCCACGGAAGCAGATGaaa                                                                                                                                       >  4:205498/1‑147 (MQ=255)
                    aCTTGGCTCACACCGGGTCGGCGTAGCTGTCATAACCGGTCAGCATGAAGGCCTGTCAGCGACAGACGCCGAAGTCCTTTTCTTTCTCGTCGCAGTAGCGGCACGGTTCGCGCATACACTCATACCCACGGAAGCGGtt                                                                                                                                           >  6:316693/1‑139 (MQ=255)
                    aCTTGGAGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGtt                                                                                                                                           <  5:316693/139‑1 (MQ=255)
                      ttGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTATCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGa                                                                                                                               >  1:102089/1‑149 (MQ=255)
                         gcgcACACCGGGTAGGCGTTGCTTGCATAACCGGTCAGCATGAAGGCCTGGCAGCGAAAGCCGCCGAAGTCCTTTTCTTTCTCGTAGCAGGAGCGGCACGGTTAGCGCATACAATCATAGCCACGGAAGCGGTTGAAACCGACCTAtt                                                                                                                             >  4:222227/1‑148 (MQ=255)
                             acacCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATAc                                                                                                                        <  5:173136/149‑1 (MQ=255)
                               acCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATAc                                                                                                                        >  5:85274/1‑147 (MQ=255)
                                ccGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATa                                                                                                                         >  2:208919/1‑145 (MQ=255)
                                ccGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATa                                                                                                                         <  1:208919/145‑1 (MQ=255)
                                 cGGGTCTGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGtt                                                                                                                                           <  3:93845/126‑1 (MQ=255)
                                 cGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGa                                                                                                                    <  2:143447/149‑1 (MQ=255)
                                                   aTCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGGACTTTTCTTTCTCGTCGCAGGAGCGGCACGGATCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGt                                                                                                  >  8:186631/1‑149 (MQ=255)
                                                    tCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTc                                                                                                 <  1:14070/149‑1 (MQ=255)
                                                     cACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTg                                                                                                    >  6:107684/1‑145 (MQ=255)
                                                     cACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTg                                                                                                    <  5:107684/145‑1 (MQ=255)
                                                        cGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTg                                                                                                    >  2:116459/1‑142 (MQ=255)
                                                        cGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTg                                                                                                    <  1:116459/142‑1 (MQ=255)
                                                              gCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTc                                                                                       <  3:177416/149‑1 (MQ=255)
                                                                aTGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCgg                                                                                     <  1:10572/149‑1 (MQ=255)
                                                                aTGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCg                                                                                      >  5:102881/1‑148 (MQ=255)
                                                                 tGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCgg                                                                                     >  5:117912/1‑148 (MQ=255)
                                                                   aaGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGaaa                                                                                  <  5:330627/149‑1 (MQ=255)
                                                                   aaGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGaa                                                                                   >  7:103759/1‑148 (MQ=255)
                                                                    aGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGaaa                                                                                  <  8:103759/148‑1 (MQ=255)
                                                                    aGGCCTGGCAGCGACAGCCGCCGAAGTCCTTATCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAAc                                                                                 <  2:102089/149‑1 (MQ=255)
                                                                     ggCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACt                                                                                <  4:97665/149‑1 (MQ=255)
                                                                                gACAGCCGACGCAGTCCTTTTCTTTCTCGTCGCAGGAGCTGCACGCTTCGCGCAGCCACTCATAGCAACGGAAGCTGTTGAAACCGAACGAGTCATACCAGATATGGTGGAAGGCGTGGTCGCGGACGTTCGGAAACGGACCCGCCAGc                                                                     >  2:130483/1‑149 (MQ=255)
                                                                                   aGCCGCCGAAGTCCTTTTCTTTCTCGTCGCACGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTgg                                                                  >  5:68112/1‑149 (MQ=255)
                                                                                     ccgccgAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGACACGGAAGCGGTTGAAACCGAACGATTCATACAAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCTGCAGCTggcg                                                                >  6:29549/1‑149 (MQ=255)
                                                                                      cgccgAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCATGGAAGCGGTTGAAACCGAACGATTCa                                                                                                                           <  6:158612/89‑1 (MQ=255)
                                                                                      cgccgAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCATGGAAGCGGTTGAAACCGAACGATTCa                                                                                                                           >  5:158612/1‑89 (MQ=255)
                                                                                        ccgAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCAATCATAGCCACGGAAGCGGTTGAAACCGAACTATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTggcgggc                                                             >  2:135991/1‑149 (MQ=255)
                                                                                          gAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGgcgc                                                           <  6:85274/149‑1 (MQ=255)
                                                                                              tCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTg                                                       >  4:123383/1‑149 (MQ=255)
                                                                                                 ttttCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTgg                                                      >  6:256869/1‑147 (MQ=255)
                                                                                                   ttctttctCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCa                                                    <  6:117912/147‑1 (MQ=255)
                                                                                                   ttctttctCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAg                                                   <  5:256869/148‑1 (MQ=255)
                                                                                                     ctttctCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCATGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAggg                                                 >  1:209264/1‑148 (MQ=255)
                                                                                                               gCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTa                                       <  6:102881/148‑1 (MQ=255)
                                                                                                               gCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTAc                                      >  5:116721/1‑149 (MQ=255)
                                                                                                                cAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTAcc                                     >  8:167043/1‑149 (MQ=255)
                                                                                                                 aGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGcc                                                          <  8:123355/127‑1 (MQ=255)
                                                                                                                 aGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGcc                                                          >  7:123355/1‑127 (MQ=255)
                                                                                                                        gCACGGTTCGCGCATCCACTCATAGCCATGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCgggg                             <  2:209264/149‑1 (MQ=255)
                                                                                                                           cGGTTCGCGCATCCAATCAAAGCCACGGAATCGGTTTAAACCGAACGATTCATACCAGAAATGGTGCAAGTCTTGGTCGCGTACATTCGGAAACTGCACCCGCAGCTGGCGGGCGCCGTGGCATGGCAATGCGGTACCGTCCGGGGTGa                          <  3:222227/149‑1 (MQ=255)
                                                                                                                              ttCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGGGATgg                       >  6:246549/1‑149 (MQ=255)
                                                                                                                                 gcgcATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGTGATGGTg                     <  6:116721/148‑1 (MQ=255)
                                                                                                                                     aTCCACTCATAGCCTCGGAAGCGTTTGAAACCGAACGATTCATACCAGATATGTTGCAAGTCGTTGTCGCGTACATTCGGAAACTGCACAGGCAGCTGGCGGGCGCCTTGTCAGGGCAATGCGGTACCGTCCGGGGTGATTGTGAGGaa                <  1:151862/149‑1 (MQ=255)
                                                                                                                                          cTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCACCGTGGCAGGGCAATg                                            <  3:278461/116‑1 (MQ=255)
                                                                                                                                          cTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCACCGTGGCAGGGCAATg                                            >  4:278461/1‑116 (MQ=255)
                                                                                                                                              tAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGATACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGTGATGGTGAGGAACAGGTTGcc       <  6:68112/149‑1 (MQ=255)
                                                                                                                                               aGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCTGTACCGTCCGGGGTGATGGTGAGGAACAGGTTGccc      >  7:125941/1‑149 (MQ=255)
                                                                                                                                                 ccACGGAAGCGGTTGAAACCGAACGATTCATACCAAATATGGTGCAAGTCGTGGTCGCGTACATTCGAAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGTGATGGTGAGGAACAGGTTGccc      >  8:48292/1‑147 (MQ=255)
                                                                                                                                                   aCGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGTGATGGTGAGGAACAGGTTGCCCCAGc  >  8:86107/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATGATGCCGTGGTCGGTCGACTTGGCGCACACCGGGTCGGCGTTGCTGGCATCACCGGTCAGCATGAAGGCCTGGCAGCGACAGCCGCCGAAGTCCTTTTCTTTCTCGTCGCAGGAGCGGCACGGTTCGCGCATCCACTCATAGCCACGGAAGCGGTTGAAACCGAACGATTCATACCAGATATGGTGCAAGTCGTGGTCGCGTACATTCGGAAACTGCACCGGCAGCTGGCGGGCGCCGTGGCAGGGCAATGCGGTACCGTCCGGGGTGATGGTGAGGAACAGGTTGCCCCAGC  >  NC_002947/456716‑457011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑