Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 458,674 C→T 7.6% G36R (GGG→AGG)  pqqC ← pyrroloquinoline‑quinone synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947458,6740CT 7.6% 150.9 / 2.0 52G36R (GGG→AGG) pqqCpyrroloquinoline‑quinone synthase
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base T (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGAGCAGGCGCTGGATCCACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTGTAGAACGATTATCTGGCCTGGCACATCTC  >  NC_002947/458531‑458822
                                                                                                                                                |                                                                                                                                                    
gTCGAGCAGGCGCTGGATCCACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTgg                                                                                                                                                  >  3:125000/1‑148 (MQ=255)
      cAGGCGCTGGATCCACTCGCGGCGCCCTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCg                                                                                                                                           <  1:282178/149‑1 (MQ=255)
         gcgcTGGATCCACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCa                                                                                                                                        >  7:82074/1‑149 (MQ=255)
         gcgcTGGATCCACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCa                                                                                                                                        <  2:233573/149‑1 (MQ=255)
               gATCCACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGt                                                                                                                                  <  6:17152/149‑1 (MQ=255)
                  ccACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCATTCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCTCTGGTACATCGCCACGTGGTAc                                                                                                                                >  1:241955/1‑149 (MQ=255)
                      tCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCCCCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCGCCTGGTACATCGCCACGTGGTACGGGt                                                                                                                            >  8:77974/1‑148 (MQ=255)
                           gcgcACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTgatgga                                                                                                                      >  3:108322/1‑149 (MQ=255)
                            cgcACTTCGCGATCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTgatggat                                                                                                                     >  6:179110/1‑149 (MQ=255)
                             gcACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTgatggatg                                                                                                                    <  2:224740/149‑1 (MQ=255)
                               aCTTCGCGATCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGAtgtg                                                                                                                  <  5:179110/149‑1 (MQ=255)
                                cTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTgg                                                                                                                 <  7:88191/149‑1 (MQ=255)
                                cTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTgg                                                                                                                 >  6:31758/1‑149 (MQ=255)
                                   cgcgGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTaa                                                                                                              >  8:138878/1‑149 (MQ=255)
                                    gcgGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTaa                                                                                                              >  3:54476/1‑148 (MQ=255)
                                              cAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGGTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCTCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCtt                                                                                                   <  2:241955/149‑1 (MQ=255)
                                                   ggCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGgcgc                                                                                              >  5:285853/1‑149 (MQ=255)
                                                    gCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGgcgc                                                                                              <  2:8957/148‑1 (MQ=255)
                                                          aTCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGc                                                                                       <  5:31758/149‑1 (MQ=255)
                                                                         cATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTc                                                                           <  4:65950/147‑1 (MQ=255)
                                                                         cATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCg                                                                          <  2:225975/148‑1 (MQ=255)
                                                                          aTCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGt                                                                                                                >  4:205779/1‑109 (MQ=255)
                                                                          aTCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGt                                                                                                                <  3:205779/109‑1 (MQ=255)
                                                                          aTCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAg                                                                                          <  6:183556/131‑1 (MQ=255)
                                                                          aTCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAg                                                                                          >  5:183556/1‑131 (MQ=255)
                                                                          aTCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGc                                                                        <  7:138878/149‑1 (MQ=255)
                                                                             gggATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGTGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCCCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAgg                                                                     >  3:59334/1‑149 (MQ=255)
                                                                             gggATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAgg                                                                     <  4:54476/149‑1 (MQ=255)
                                                                              ggATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTg                                                                                    >  1:273061/1‑133 (MQ=255)
                                                                              ggATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTg                                                                                    <  2:273061/133‑1 (MQ=255)
                                                                                 tGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGAc                                                                 <  5:186686/149‑1 (MQ=255)
                                                                                      aCCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTgg                                                            >  2:154051/1‑149 (MQ=255)
                                                                                               tagAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGaa                                                                              >  8:273692/1‑122 (MQ=255)
                                                                                               tagAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGaa                                                                              <  7:273692/122‑1 (MQ=255)
                                                                                                 gAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCg                                                  <  1:154051/148‑1 (MQ=255)
                                                                                                  aaGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATCGGCAGTGCGTCGCt                                                >  7:130401/1‑149 (MQ=255)
                                                                                                    gCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCa                                              <  4:221620/149‑1 (MQ=255)
                                                                                                       gTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTc                                           <  3:94934/149‑1 (MQ=255)
                                                                                                       gTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTc                                           >  6:89780/1‑149 (MQ=255)
                                                                                                           gCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggct                                       >  1:273605/1‑149 (MQ=255)
                                                                                                           gCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggct                                       <  4:108322/149‑1 (MQ=255)
                                                                                                           gCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggct                                       >  4:277025/1‑149 (MQ=255)
                                                                                                                   gCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTg                               <  3:277025/149‑1 (MQ=255)
                                                                                                                    cccTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAg                                                                      >  4:232338/1‑109 (MQ=255)
                                                                                                                    cccTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAg                                                                      <  3:232338/109‑1 (MQ=255)
                                                                                                                     ccTGGATCTGCTCGCGGGTGGCGCGCCTCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggctgg                                     >  4:170338/1‑141 (MQ=255)
                                                                                                                     ccTGGATCTGCTCGCGGGTGGCGCGCCTCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggctgg                                     <  3:170338/141‑1 (MQ=255)
                                                                                                                         gATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggctgg                                     <  4:213985/137‑1 (MQ=255)
                                                                                                                         gATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCggctgg                                     >  3:213985/1‑137 (MQ=255)
                                                                                                                               cTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTGTAGAACGATTAt                   >  3:130272/1‑149 (MQ=255)
                                                                                                                                tCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTGTAGAACGATTATc                  >  2:250145/1‑149 (MQ=255)
                                                                                                                                            cgcCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTGTAGAACGATTATCTGGCCTGGcaca      <  2:273605/149‑1 (MQ=255)
                                                                                                                                                ccTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTGTAGAACGATTATCTGGCCTGGCACAtctc  >  7:12693/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GTCGAGCAGGCGCTGGATCCACTCGCGGCGCACTTCGCGGTCCGGGCAGTTGGCCAGGATCGCGGCA‑TCCTTCATCGGGATGTTGACCTGGTAGTAGAAGCGGTTGGCCACCCAGCCCTGGATCTGCTCGCGGGTGGCGCGCCCCTGGTACATCGCCACGTGGTACGGGTGATGGATGTGGTAATAGGCGCCCTTGGCGCGCAGGGCCTGCTCGAACTCGGCAGGGGACATTGGCAGTGCGTCGCTCATTCGGCTGGCTCCTGTAGAACGATTATCTGGCCTGGCACATCTC  >  NC_002947/458531‑458822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑