Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,579,597	T→G	11.3%	intergenic (+56/+20)	psd → / ← serB	phosphatidylserine decarboxylase/phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,579,597	0	T	G	11.3%	172.1 / 12.4	62	intergenic (+56/+20)	psd/serB	phosphatidylserine decarboxylase/phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (26/29);  new base G (3/4);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TTCGGGCCAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTGGCACGAAACGCCACGCCCAACCCAG  >  NC_002947/5579449‑5579744                                                                                                                                                     |                                                                                                                                                     ttCGGGCCAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCtt                                                                                                                                                      >  1:68577/1‑149 (MQ=255)     ggCCAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTAGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCgg                                                                                                                                                  >  3:124031/1‑149 (MQ=255)      gCCAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGc                                                                                                                                                 <  7:303871/149‑1 (MQ=255)       ccAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCTGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTGGGGCCGCAAGGCGGcc                                                                                                                                                >  4:289212/1‑149 (MQ=255)       ccAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCTGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTGGGGCCGCAAGGCGGcc                                                                                                                                                >  4:294022/1‑149 (MQ=255)          gagCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCAAGGCGGcccaa                                                                                                                                             <  8:296245/149‑3 (MQ=255)           agCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCTGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTGGGGCCGCAAGGCGGcccaac                                                                                                                                            <  3:294022/149‑4 (MQ=255)           agCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCTGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTGGGGCCGCAAGGCGGcccaac                                                                                                                                            <  3:289212/149‑4 (MQ=255)                 tGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCAc                                                                                                                                      >  6:221403/1‑149 (MQ=255)                 tGAAAGGGGCTGAGCGCCTGGGGGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCAc                                                                                                                                      <  2:236037/149‑1 (MQ=255)                             agagCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGcccc                                                                                                                                              >  7:256764/1‑129 (MQ=255)                             agagCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGcccc                                                                                                                                              <  8:256764/129‑1 (MQ=255)                               agCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCggg                                                                                                                        <  5:244380/149‑1 (MQ=255)                                  cTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGt                                                                                                                     >  5:29561/1‑149 (MQ=255)                                    gggTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTcg                                                                                                                   >  3:217416/1‑149 (MQ=255)                                         cAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATc                                                                                                              <  2:68577/149‑1 (MQ=255)                                           ggCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCAc                                                                                                            >  5:151956/1‑149 (MQ=255)                                            gCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGCTACGCCGCGGGCGTCGCGATCACg                                                                                                           >  4:16190/1‑149 (MQ=255)                                              tctGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGa                                                                                                                >  7:65275/1‑142 (MQ=255)                                              tctGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGa                                                                                                                <  8:65275/142‑1 (MQ=255)                                                tGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGt                                                                                                                     >  5:7200/1‑135 (MQ=255)                                                tGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGt                                                                                                                     <  6:7200/135‑1 (MQ=255)                                                tGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑gg                                                                                                      >  4:245892/1‑149 (MQ=255)                                                 gCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGc                                                                                                     >  3:179608/1‑149 (MQ=255)                                                       cGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGa                                                                                                                                           >  1:258381/1‑106 (MQ=255)                                                       cGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGa                                                                                                                                           <  2:258381/106‑1 (MQ=255)                                                        gCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCcagca                                                                                               <  4:99620/148‑1 (MQ=255)                                                             gggCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTAc                                                                                          <  5:221403/148‑1 (MQ=255)                                                             gggCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTAc                                                                                          <  5:181906/148‑1 (MQ=255)                                                                  gcagcTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCAcc                                                                                    <  4:293725/149‑1 (MQ=255)                                                                         gcggcgCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCa                                                                             <  4:217416/149‑1 (MQ=255)                                                                          cggcgCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCAc                                                                                                                                      >  6:87991/1‑92 (MQ=255)                                                                          cggcgCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCAc                                                                                                                                      <  5:87991/92‑1 (MQ=255)                                                                            gcggCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGGCCTGCATATGGAGGGCCTTTTTCGTTGGGGCGCCTTGCGGCCCCGGTTTCACGGCTCAGCCGCGGGCGTCGCGATCACGCATGCCCCAGCAGGTACAGCACCCCGTCCAGc                                                                           <  1:4526/149‑1 (MQ=255)                                                                                        gCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGaaa                                                              >  5:309174/1‑149 (MQ=255)                                                                                          ttGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATc                                                            >  3:78051/1‑149 (MQ=255)                                                                                             aTTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGc                                                          <  6:29561/148‑1 (MQ=255)                                                                                               tCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCt                                                        <  4:124031/148‑1 (MQ=255)                                                                                                cGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGc                                                      >  1:319816/1‑149 (MQ=255)                                                                                                 gAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCt                                                     >  4:86644/1‑149 (MQ=255)                                                                                                    ccATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTgg                                                  <  4:78051/149‑1 (MQ=255)                                                                                                        atGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGa                                              >  3:77100/1‑149 (MQ=255)                                                                                                          gAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGCCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACt                                            >  8:127620/1‑149 (MQ=255)                                                                                                           aaaaGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACt                                            <  1:78578/148‑1 (MQ=255)                                                                                                             aaGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTg                                           <  3:265767/147‑1 (MQ=255)                                                                                                             aaGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTg                                           <  3:261229/147‑1 (MQ=255)                                                                                                             aaGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTg                                           >  4:265767/1‑147 (MQ=255)                                                                                                             aaGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTg                                           >  4:261229/1‑147 (MQ=255)                                                                                                                  ccTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCAcc                                    <  4:77100/149‑1 (MQ=255)                                                                                                                  ccTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCAc                                     >  2:67711/1‑148 (MQ=255)                                                                                                                          tGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCtt                            <  6:309174/149‑1 (MQ=255)                                                                                                                            cAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTgg                          <  4:179608/149‑1 (MQ=255)                                                                                                                            cAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTg                           <  3:86644/148‑1 (MQ=255)                                                                                                                                   tttttCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAg                                                                     <  4:203909/99‑1 (MQ=255)                                                                                                                                   tttttCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAg                                                                     >  3:203909/1‑99 (MQ=255)                                                                                                                                       atccggggCCGCAAGGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTg                           >  4:47832/6‑137 (MQ=255)                                                                                                                                       atccggggCCGCAAGGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTg                           <  3:47832/132‑1 (MQ=255)                                                                                                                                        cGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTGGCACGAAacgcca              >  7:99942/1‑149 (MQ=255)                                                                                                                                         gTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTgg                          >  7:213295/1‑136 (MQ=255)                                                                                                                                         gTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTgg                          <  8:213295/136‑1 (MQ=255)                                                                                                                                               ccgccTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTgg                          <  2:193860/130‑1 (MQ=255)                                                                                                                                               ccgccTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTgg                          >  1:193860/1‑130 (MQ=255)                                                                                                                                                   cTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTGGCACGAAACGCCACGCCCAACcca   <  2:319816/149‑1 (MQ=255)                                                                                                                                                    ttGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTGGCACGAAACGCCACGCCCAACccag  >  6:28636/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TTCGGGCCAGAGCAGGTGAAATGGGCTGAGAGCCTGGGTGCAGGCTCTGCAGTTCGCATGGGGCAGCAGCTGGCGGCGCCTGCGCAGGCTTGATTCGAGCCATATGAAAAGGCCCTGCATATGCAGGGCCTTTTTCGTTGGGCCGCCTTGCGGCCCCGGATTCACGGATCAGCCGCGGGCGTCGCGATCACGCA‑GGCCCAGCAGGTACAGCACCCCGTCCAGCCCCAAGGTGGAAATCGCCTGCTTGGCCGACTGGCGCACCAGCGGCTTGGCACGAAACGCCACGCCCAACCCAG  >  NC_002947/5579449‑5579744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑