| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,608,300 | G→C | 10.4% | intergenic (‑162/+26) | cyaB ← / ← waaA | cyclolysin secretion/processing ATP‑binding protein CyaB/3‑deoxy‑D‑manno‑octulosonic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,608,300 | 0 | G | C | 10.4% | 101.0 / 2.4 | 37 | intergenic (‑162/+26) | cyaB/waaA | cyclolysin secretion/processing ATP‑binding protein CyaB/3‑deoxy‑D‑manno‑octulosonic acid transferase |
| Reads supporting (aligned to +/- strand): ref base G (13/20); new base C (2/2); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
CACAAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACC > NC_002947/5608153‑5608440 | cacaAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCgg < 8:84538/149‑1 (MQ=255) acaAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGGGAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCgg > 1:80293/1‑149 (MQ=255) caAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGt < 1:255052/149‑1 (MQ=255) cTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCttt < 2:80293/149‑1 (MQ=255) gCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TCAAAGACCCGGTC‑TAGAGTGGat < 5:180865/149‑1 (MQ=39) gCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGat < 8:91193/149‑1 (MQ=255) gCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGat < 5:157062/149‑1 (MQ=255) gCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGat < 7:205437/149‑1 (MQ=255) cAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TCAAAGACCCGGTC‑TAGAGTGGata < 5:132489/149‑1 (MQ=39) gtgtGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCg < 2:300246/149‑1 (MQ=255) cTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGGGGATATGTCGGTTATCAg < 6:242532/149‑1 (MQ=255) aCTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCATTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCACACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCgg < 4:42883/146‑1 (MQ=255) ttAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAg < 1:285589/148‑1 (MQ=255) cTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGccccct > 2:250587/1‑147 (MQ=255) aaGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAg < 1:96086/148‑1 (MQ=255) gagaAATGCAGTGTATGAAGTTACAACTTACGCCGTAGTAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGTGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGc < 4:245550/142‑1 (MQ=255) gagaAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TATACCCGCTCTTTGAGTGGATATGTCGGATATCACCGGATCAAGCCCCCCAGAACCTCGAGCAATCGATGCaagatcg > 3:245550/1‑144 (MQ=255) gagaAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGGCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGccc < 2:256069/149‑1 (MQ=255) aaGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATc < 8:213894/149‑1 (MQ=255) tACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTc < 8:164456/108‑1 (MQ=255) tACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTc > 7:164456/1‑108 (MQ=255) cTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCg < 6:329824/136‑1 (MQ=255) cTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCg > 5:329824/1‑136 (MQ=255) aCGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGccc > 1:253277/1‑149 (MQ=255) gCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGGCCCCAGGCCCTCGAGCAATCGCTGCACAGCCCCCTGCGTGGCCCGCATCACCGCCCTGCCCGc > 7:50216/1‑149 (MQ=255) aataaataACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCa > 3:277178/1‑149 (MQ=255) taaataaCCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCAc < 2:74197/144‑1 (MQ=255) taaataaCCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCAc > 1:74197/1‑144 (MQ=255) aCCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTgg > 4:11197/1‑149 (MQ=255) ccAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTTCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTggg < 1:250587/149‑1 (MQ=255) cAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGccc < 7:164072/112‑1 (MQ=255) cAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGccc > 8:164072/1‑112 (MQ=255) aaTCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTc > 2:66550/1‑149 (MQ=255) aTCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTcc < 2:253277/149‑1 (MQ=255) tCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCgg > 2:297551/1‑144 (MQ=255) tCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCgg < 1:297551/144‑1 (MQ=255) ccAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTc > 3:265307/1‑149 (MQ=255) cactcaaaGACCCGGTC‑TAGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTc > 6:68694/8‑148 (MQ=255) tc‑tAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGCCCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCACGCCTCCCCCATGAGCTGGGCGTCCTGCGGCAGTTccacc > 3:159621/1‑149 (MQ=255) | CACAAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTC‑GTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCAC‑TC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACC > NC_002947/5608153‑5608440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |