Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,609,000 G→C 7.9% P200A (CCT→GCT)  waaA ← 3‑deoxy‑D‑manno‑octulosonic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,609,0000GC 7.9% 232.5 / 7.1 75P200A (CCT→GCT) waaA3‑deoxy‑D‑manno‑octulosonic acid transferase
Reads supporting (aligned to +/- strand):  ref base G (34/35);  new base C (3/3);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCAG  >  NC_002947/5608852‑5609144
                                                                                                                                                    |                                                                                                                                                
gCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAgg                                                                                                                                                  <  5:34774/149‑1 (MQ=255)
      tCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTg                                                                                                                                             >  1:288411/1‑148 (MQ=255)
       cACCGTCGTGGGTGCTGGCGTCAATCCACTCAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                              <  5:253835/146‑1 (MQ=255)
       cACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCTACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGc                                                                                                                                            <  5:281050/148‑1 (MQ=255)
       cACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCg                                                                                                                                               <  5:167941/145‑1 (MQ=255)
         ccGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCAcc                                                                                                                                         >  1:124944/1‑149 (MQ=255)
          cgtcgtGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCAcc                                                                                                                                         >  8:162505/1‑148 (MQ=255)
          cgtcgtGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaagt                                                                                                                                        <  2:288411/149‑4 (MQ=255)
                gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCa                                                                                                                                  >  7:147660/1‑149 (MQ=255)
                gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCa                                                                                                                                  <  7:162505/149‑1 (MQ=255)
                gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                          <  5:93674/141‑2 (MQ=255)
                gggTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                          >  6:93674/1‑140 (MQ=255)
                     cTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTTCACGCGCTCAGGGCGTGCACCCAGGTCACGGaa                                                                                                                             <  8:197664/149‑1 (MQ=255)
                                ccACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACgg                                                                                                                               >  8:129952/1‑136 (MQ=255)
                                ccACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACgg                                                                                                                               <  7:129952/136‑1 (MQ=255)
                                ccACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACATGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCt                                                                                                                  >  4:138702/1‑149 (MQ=255)
                                 cacacaGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTc                                                                                                                 <  1:203911/149‑1 (MQ=255)
                                   cacaGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCgg                                                                                                               >  7:149656/1‑149 (MQ=255)
                                        ggCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCTAACTTGATCGAGCCGGTGAGTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCg                                                                                                          <  2:24861/149‑1 (MQ=255)
                                        ggCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCg                                                                                                          >  6:74402/1‑149 (MQ=255)
                                        ggCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGGTTCg                                                                                                          >  2:201275/1‑149 (MQ=255)
                                        ggCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGGCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCg                                                                                                          <  5:311116/149‑1 (MQ=255)
                                          cgcTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGt                                                                                                        >  2:15364/1‑149 (MQ=255)
                                           gcTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                       >  3:167345/1‑149 (MQ=255)
                                           gcTGGCCGCCCCCAAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                       >  2:227872/1‑149 (MQ=255)
                                               gccgccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGc                                                                                                    <  8:149656/148‑1 (MQ=255)
                                               gccgccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGc                                                                                                    <  1:15364/148‑1 (MQ=255)
                                                 cgccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACg                                                                                                 >  5:48034/1‑149 (MQ=255)
                                                  gccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACgg                                                                                                >  5:92139/1‑149 (MQ=255)
                                                              ttCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCTTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGc                                                                                                    <  7:292558/133‑1 (MQ=255)
                                                              ttCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGc                                                                                                    >  8:292558/1‑133 (MQ=255)
                                                               gcACGCACTGCCCGCGCGGGGGGCTGCCGCTGTTCGGCGATGGTCATGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCAt                                                                                   <  8:203564/148‑1 (MQ=255)
                                                                 aCGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCAtct                                                                                 >  4:150270/1‑149 (MQ=255)
                                                                    cAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCgg                                                                              <  3:254438/149‑1 (MQ=255)
                                                                                  gTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCgg                                                                <  4:7563/149‑1 (MQ=255)
                                                                                     gcagcaGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCg                                                                 <  7:201437/145‑1 (MQ=255)
                                                                                     gcagcaGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCg                                                                 >  8:201437/1‑145 (MQ=255)
                                                                                      cagcagCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCa                                                            <  5:211002/149‑1 (MQ=255)
                                                                                        gcagcTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGc                                                          <  3:150270/149‑1 (MQ=255)
                                                                                          agcTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGc                                                          >  4:135143/1‑147 (MQ=255)
                                                                                          agcTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGc                                                          <  3:135143/147‑1 (MQ=255)
                                                                                           gcTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTg                                                       <  4:141403/149‑1 (MQ=255)
                                                                                           gcTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTg                                                       >  5:216845/1‑149 (MQ=255)
                                                                                            cTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTgg                                                      <  2:124944/149‑1 (MQ=255)
                                                                                             tGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCggg                                                               >  5:77063/1‑139 (MQ=255)
                                                                                             tGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCggg                                                               <  6:77063/139‑1 (MQ=255)
                                                                                              gTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                     >  4:229496/1‑148 (MQ=255)
                                                                                                  gtcgATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                     >  5:313830/1‑144 (MQ=255)
                                                                                                  gtcgATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                     <  6:313830/144‑1 (MQ=255)
                                                                                                  gtcgATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgc                                                >  2:222416/1‑149 (MQ=255)
                                                                                                     gATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgcgc                                              <  4:167345/148‑1 (MQ=255)
                                                                                                      agggTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGcc                                             >  1:198429/3‑148 (MQ=255)
                                                                                                         gTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATa                                         <  5:70777/149‑1 (MQ=255)
                                                                                                           cAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCACTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGc                                       >  7:252015/1‑149 (MQ=255)
                                                                                                              gTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGcc                                             <  6:152447/140‑1 (MQ=255)
                                                                                                              gTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGcc                                             >  5:152447/1‑140 (MQ=255)
                                                                                                              gTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCAc                                    >  6:2375/1‑149 (MQ=255)
                                                                                                              gTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCAc                                    <  8:147660/149‑1 (MQ=255)
                                                                                                                 gAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACggg                                 <  6:92139/149‑1 (MQ=255)
                                                                                                                  aaCTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGc                                <  2:2148/149‑1 (MQ=255)
                                                                                                                    cTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGcc                               >  3:243122/1‑148 (MQ=255)
                                                                                                                     ttGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCGTCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGcc                               >  1:137051/1‑147 (MQ=255)
                                                                                                                     ttGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCACTATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGa                             <  1:227872/149‑1 (MQ=255)
                                                                                                                         tCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAgcgc                         >  3:7517/1‑149 (MQ=255)
                                                                                                                         tCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAgcgc                         >  8:76773/1‑149 (MQ=255)
                                                                                                                              ccGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGa                    >  8:11336/1‑149 (MQ=255)
                                                                                                                                ggTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGAc                   >  5:202678/1‑148 (MQ=255)
                                                                                                                                    aCTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgcg              <  1:222416/149‑1 (MQ=255)
                                                                                                                                     ggtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAg                            <  7:43800/132‑1 (MQ=255)
                                                                                                                                     ggtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAg                            >  8:43800/3‑134 (MQ=255)
                                                                                                                                      ttGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgcgcg            >  1:218327/1‑149 (MQ=255)
                                                                                                                                          aCGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgcgc                                              <  2:235122/111‑1 (MQ=255)
                                                                                                                                          aCGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgcgc                                              >  1:235122/1‑111 (MQ=255)
                                                                                                                                           cgcgCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTg       <  4:7517/149‑1 (MQ=255)
                                                                                                                                           cgcgCTCAGGGCGTGCACCCAGGGCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTg       <  3:229496/149‑1 (MQ=255)
                                                                                                                                                  aGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCAg  <  5:74402/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGATCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTGCACGGCGATCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTGGCCAG  >  NC_002947/5608852‑5609144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑