Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,622,932	A→C	10.9%	M269R (ATG→AGG) ‡	PP_4941 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,622,932	0	A	C	10.9%	177.0 / 11.3	63	M269R (ATG→AGG) ‡	PP_4941	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base C (4/3);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGG  >  NC_002947/5622787‑5623078                                                                                                                                                  |                                                                                                                                                   ggCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATgg                                                                                                                                                 <  6:49336/149‑1 (MQ=255)    tCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggcag                                                                                                                                              >  2:129960/1‑149 (MQ=255)      gCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggcag                                                                                                                                              <  1:129960/147‑1 (MQ=255)      gCAGGTCGGCCAGCAGGGTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggcaggc                                                                                                                                            <  7:242107/149‑1 (MQ=255)                      gTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAg                                                                                                                             >  5:274644/1‑148 (MQ=255)                         gtgCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAg                                                                                                                          <  5:18756/148‑1 (MQ=255)                         gtgCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGc                                                                                                                         <  4:212691/149‑1 (MQ=255)                          tgCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGTAGGCAAAAGTAGcc                                                                                                                        <  8:39095/149‑1 (MQ=255)                            ccAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggca                                                                                                                                               >  1:28386/1‑124 (MQ=255)                            ccAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggca                                                                                                                                               <  2:28386/124‑1 (MQ=255)                            ccAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAg                                                                                                                          <  6:154070/145‑1 (MQ=255)                            ccAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAg                                                                                                                          >  5:154070/1‑145 (MQ=255)                             cAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCCATGCAGGCAGca                                                                                                                                        <  7:123232/130‑2 (MQ=255)                               ggtgggtgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCt                                                                                                                   <  5:237124/149‑1 (MQ=255)                                 tgggtgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGt                                                                                                                 <  6:274644/149‑1 (MQ=255)                                    gtgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATg                                                                                                              <  6:327460/149‑1 (MQ=255)                                       cgcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCaa                                                                                                           <  1:185182/149‑1 (MQ=255)                                              gcgGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACt                                                                                                    >  3:106108/1‑149 (MQ=255)                                               cgGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTc                                                                                                   >  7:239440/1‑149 (MQ=255)                                                    gCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTTCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCa                                                                                              <  8:239440/149‑1 (MQ=255)                                                    gCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCa                                                                                              >  2:259371/1‑149 (MQ=255)                                                      ccGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACg                                                                                            >  2:173498/1‑149 (MQ=255)                                                          tAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCgg                                                                                        >  6:185579/1‑149 (MQ=255)                                                            acgggaacgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGc                                                                                       <  4:106108/148‑1 (MQ=255)                                                              gggaacgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATg                                                                                                                                                  >  7:282534/1‑87 (MQ=255)                                                              gggaacgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATg                                                                                                                                                  <  8:282534/87‑1 (MQ=255)                                                                      gTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGc                                                                                                             >  1:232258/1‑116 (MQ=255)                                                                      gTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGc                                                                                                             <  2:232258/116‑1 (MQ=255)                                                                      gTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCa                                                                            >  6:39006/1‑149 (MQ=255)                                                                      gTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCa                                                                            >  4:194112/1‑149 (MQ=255)                                                                          aaCTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCacgac                                                                        >  2:154389/1‑149 (MQ=255)                                                                               gCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATg                                                                    <  5:39006/148‑1 (MQ=255)                                                                               gCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGc                                                                   >  5:176791/1‑149 (MQ=255)                                                                                  gAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGt                                                                <  1:259371/149‑1 (MQ=255)                                                                                      acGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTcc                                                              >  1:17391/1‑147 (MQ=255)                                                                                      acGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCa                                                            <  6:176791/149‑1 (MQ=255)                                                                                         cgGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGGCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGc                                                         <  2:216387/149‑1 (MQ=255)                                                                                                cGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTAGGGTGCTGCCAGCATGGCAGGCAGCTGCATGGAAAAGTAGCCCAGCTGTATTCAACGCGACTCCCGCACGCCGGCCCAACGCGGGACCACGATGCTGTCCCACGAACGCAgg                                                  >  2:302294/1‑149 (MQ=255)                                                                                                   ccAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGgcgc                                               <  3:194112/149‑1 (MQ=255)                                                                                                        tCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCtt                                          >  4:64727/1‑149 (MQ=255)                                                                                                        tCGGCTTCACGTGAGGCGGCCGGCTGGACGGGTGCTGCCAGAATGGCAGGAAGCGGCAGCGAAAAATAGCCAAGCAGTATGCAAACCGACTACGGCAAGCCGGCCAAACCCGGCACGCCGAAGCATTCCCAGGCACGCCGGCccccaa                                           >  6:44648/1‑146 (MQ=255)                                                                                                          ggCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTc                                         <  3:209865/148‑1 (MQ=255)                                                                                                               cACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgcgc                                   >  7:98189/1‑149 (MQ=255)                                                                                                                 cGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgcgcgc                                 <  8:98189/149‑1 (MQ=255)                                                                                                                  gTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGcgcgcg                                <  3:64727/149‑1 (MQ=255)                                                                                                                   tGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcgg                               >  3:77583/1‑149 (MQ=255)                                                                                                                        cggccggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcg                          <  4:77583/149‑1 (MQ=255)                                                                                                                                tGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGc                  <  1:154389/149‑1 (MQ=255)                                                                                                                                   tCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAg               <  2:51098/149‑1 (MQ=255)                                                                                                                                    ctgcagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAg                                       <  4:247142/119‑1 (MQ=255)                                                                                                                                    ctgcagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAg                                       >  3:247142/6‑124 (MQ=255)                                                                                                                                     gggTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc             <  1:173498/149‑1 (MQ=255)                                                                                                                                      gcagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc             <  4:166902/145‑1 (MQ=255)                                                                                                                                      gcagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGc             >  3:166902/4‑148 (MQ=255)                                                                                                                                       cagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGcc            >  2:123578/3‑148 (MQ=255)                                                                                                                                         gctgcCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCAt         >  3:8087/1‑149 (MQ=255)                                                                                                                                          ctgcctgcCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCCGGCACGCAGGCGCCCCGTCAGGCGCGCGGCCTCGGCCTTTGCCCGGCCCATc        >  7:258735/1‑149 (MQ=255)                                                                                                                                            gcctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGc                                                     >  6:95834/1‑102 (MQ=255)                                                                                                                                              ctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgct    <  5:185579/149‑1 (MQ=255)                                                                                                                                               tgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgctg   >  7:219880/1‑149 (MQ=255)                                                                                                                                                gcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTgg  >  5:119276/1‑149 (MQ=255)                                                                                                                                                 cATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCAt         >  7:143910/1‑141 (MQ=255)                                                                                                                                                 cATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCAt         <  8:143910/141‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGG  >  NC_002947/5622787‑5623078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑