Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,634,457 T→G 14.9% intergenic (+33/+350) putA → / ← PP_5718 proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,634,4570TG14.9% 118.2 / 10.6 46intergenic (+33/+350)putA/PP_5718proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (20/19);  new base G (3/4);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGTCCAGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTC  >  NC_002947/5634312‑5634603
                                                                                                                                                 |                                                                                                                                                   
ggCTGTCCAGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCgg                                                                                                                                                  <  6:130294/149‑1 (MQ=255)
      ccAGCGGGGATGTCCTGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGGGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTTAAGAAGAAGGAGAGCCTCGGCtctc                                                                                                                                             <  4:24463/148‑1 (MQ=255)
        aGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑tt                                                                                                                                         >  1:81132/1‑149 (MQ=255)
         gCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑ttt                                                                                                                                        >  5:80385/1‑149 (MQ=255)
         gCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTCC‑ttc                                                                                                                                        <  6:143154/149‑2 (MQ=255)
           ggggATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑ttttt                                                                                                                                      <  4:312712/149‑1 (MQ=255)
            gggATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTCc‑ttcttc                                                                                                                                     <  8:245847/149‑2 (MQ=255)
                 cACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTCC‑tt                                                                                                                                         >  3:115298/1‑140 (MQ=255)
                 cACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGAGGCTCTCC‑tt                                                                                                                                         <  4:115298/140‑1 (MQ=255)
                   ccAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑tttt                                                                                                                                       <  4:99342/140‑1 (MQ=255)
                   ccAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑tttt                                                                                                                                       >  3:99342/1‑140 (MQ=255)
                    cAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTTATGCGt                                                                                                                               >  7:44588/1‑148 (MQ=255)
                        ttGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATg                                                                                                                          >  4:2978/1‑148 (MQ=255)
                            gcTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCAAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCt                                                                                                                      >  7:204466/1‑148 (MQ=255)
                              tGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTg                                                                                                                     >  4:10041/1‑147 (MQ=255)
                                gAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGc                                                                                                                 <  3:2978/149‑1 (MQ=255)
                                   cgcCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAg                                                                                                               <  7:113893/148‑1 (MQ=255)
                                     ccTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAgggg                                                                                                            >  4:58610/1‑149 (MQ=255)
                                      cTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGt                                                                                                                               >  7:165717/1‑129 (MQ=255)
                                      cTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGt                                                                                                                               <  8:165717/129‑1 (MQ=255)
                                          tCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGa                                                                                                       <  3:10041/149‑1 (MQ=255)
                                          tCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGa                                                                                                       >  5:180188/1‑149 (MQ=255)
                                             tCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGa                                                                                                       >  7:25985/1‑146 (MQ=255)
                                             tCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGa                                                                                                       <  8:25985/146‑1 (MQ=255)
                                                  cgcgcgGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAgg                                                                                               <  2:237529/149‑1 (MQ=255)
                                                      cgGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCt                                                                                           >  3:204419/1‑149 (MQ=255)
                                                            gCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCCTTTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCa                                                                                      <  8:44588/149‑1 (MQ=255)
                                                                        cAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTATTGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCGCGGCTCTCC‑TTTTTTATGGGTGCATGTCCTGAAGCTGGGGCTGGCGTAACAGGTGGTCACAAGGGCGTGGTCATg                                                                         >  8:129521/1‑149 (MQ=255)
                                                                        cAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCAt                                                                          <  4:204419/148‑1 (MQ=255)
                                                                              cggGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACg                                                                   >  6:145087/1‑149 (MQ=255)
                                                                                    gtAACGTCATCCTGATGTTCTTTGGCTATTTGTTGTTGGTTTTTTTAGAAGAAGGTGATCCTCTGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCAGCAGAACGTTGTCATGCCAACGGTGAc                                                              <  8:204466/148‑1 (MQ=255)
                                                                                         gCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGgt                                                        <  2:81132/149‑1 (MQ=255)
                                                                                              ccTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGtctt                                                   <  3:58610/149‑1 (MQ=255)
                                                                                                  atgaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGCAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCtg                                                <  6:180188/148‑1 (MQ=255)
                                                                                                   tgaCCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCtgtt                                              <  5:145087/149‑1 (MQ=255)
                                                                                                                         ggTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTg                            <  4:257834/145‑1 (MQ=255)
                                                                                                                         ggTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTg                            >  3:257834/1‑145 (MQ=255)
                                                                                                                                gaagaagaaGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCg                  >  4:311778/1‑148 (MQ=255)
                                                                                                                                 ataaaaaagGAGAGCCGAGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGc                >  6:152611/7‑149 (MQ=255)
                                                                                                                                 aagaagaagGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCg                  <  5:320973/147‑1 (MQ=255)
                                                                                                                                 aagaagaagGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCg                  >  6:320973/1‑147 (MQ=255)
                                                                                                                                      gaagGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCt           >  6:69899/1‑149 (MQ=255)
                                                                                                                                      gaagGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGATCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCt           >  2:93998/1‑149 (MQ=255)
                                                                                                                                         ggAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTggg        >  2:50742/1‑149 (MQ=255)
                                                                                                                                            gagCCGAGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCg     <  5:152611/149‑1 (MQ=255)
                                                                                                                                               ccTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTc  >  3:147323/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGCTGTCCAGCGGGGATCACCAGATTGCGCTGGAGCGCCTGGTCATCGAGCGCGCGGTGAGCGTGAACACTGCAGCGGCGGGTGGTAACGCCAGCCTGATGACCATTGGCTAATGGCTGAGGGTTGTTGAAGAAGAAGGAGAGCCTCGGCTCTCC‑TTTTTTATGCGTGCATGTCCTGAAGCAGGGGCTGGACTAACAGGTGCTCACCAGAACGTGGTCATGCCAACGGTGACTTGGGTGTCTTCTGTTGTTACCGTTTATCCCCTGGAAATTCTCGGCTGCCTGGGCCGGTC  >  NC_002947/5634312‑5634603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑