Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	464,322	G→C	7.7%	H259D (CAC→GAC) ‡	davB ←	lysine 2‑monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	464,322	0	G	C	7.7%	195.8 / 6.7	65	H259D (CAC→GAC) ‡	davB	lysine 2‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base C (2/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

GCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTG  >  NC_002947/464174‑464470                                                                                                                                                        |                                                                                                                                                     GCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTg                                                                                                                                                      <  2:269758‑M1/149‑1 (MQ=255)    GCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCtg                                                                                                                                                   >  7:268710‑M1/1‑149 (MQ=255)       CGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCtggtg                                                                                                                                                >  4:230090‑M1/1‑149 (MQ=255)        gggCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCtggtgg                                                                                                                                               >  7:160120/1‑149 (MQ=255)           cAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcg                                                                                                                                            <  2:295496/149‑1 (MQ=255)                cgcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTGCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCa                                                                                                                                       <  5:231539/149‑1 (MQ=255)                      aCCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCTCTTGGt                                                                                                                                 >  7:298716/1‑149 (MQ=255)                       ccgcGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtc                                                                                                                                <  5:212838/146‑1 (MQ=255)                        cccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                         <  8:187125/139‑1 (MQ=255)                        cccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                         >  7:187125/1‑139 (MQ=255)                        cccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtca                                                                                                                               >  5:118436/1‑149 (MQ=255)                         ccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtcat                                                                                                                              <  3:52871/149‑1 (MQ=255)                             tACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATcacc                                                                                                                          <  6:241007/149‑1 (MQ=255)                               ttgcccgcgccgccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATcaccac                                                                                                                        <  8:86570‑M2/135‑1 (MQ=255)                                gcgGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCAcg                                                                                                                       >  4:119187/1‑149 (MQ=255)                                      cgccgccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGaa                                                                                                                  <  3:82582‑M2/141‑1 (MQ=255)                                             atggcgCGTGCTCAGGGGTGGGCCCTCTGGCCAAATCACGGATCGGTTCGGTACGTCGGGCCATATTCCTTGTT‑GGACCAAAGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                            <  1:100135/144‑1 (MQ=255)                                                  gCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATc                                                                                                     <  6:118436/149‑1 (MQ=255)                                                    gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATc                                                                                                     >  6:238487/1‑147 (MQ=255)                                                       cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCa                                                                                                          >  4:207322/1‑139 (MQ=255)                                                       cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCa                                                                                                          <  3:207322/139‑1 (MQ=255)                                                         cAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                              >  3:22759/1‑149 (MQ=255)                                                          aGGCTTGTGCCCGCTGGCCAATGCACGCAGCATTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCTACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGCGTTgg                                                                                              <  2:233197/148‑1 (MQ=255)                                                          aGGCTTGTGCACTGTGTCCAATGAACTCATCTTTCCGGTAAGTCGCGTCAGATGCCTTGTG‑GCAC‑‑TTGTTGCAGGCTCCCGAGGACCTGTTGCTGGTGGTCTTCGCAGTTGGTCTTTACCACTTGTAAGATTTCCAGCATCTAGTTgg                                                                                              <  8:160120/148‑1 (MQ=255)                                                                 tggccTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCGTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                      <  4:280962/146‑1 (MQ=255)                                                                   cccTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGa                                                                                    <  1:269758/149‑1 (MQ=255)                                                                        tGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                 <  4:17058/147‑1 (MQ=255)                                                                           ccAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                            <  5:235386/149‑1 (MQ=255)                                                                                   cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTg                                                                    >  5:260768/1‑149 (MQ=255)                                                                                    gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGTGCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTg                                                                    >  1:48880/1‑149 (MQ=255)                                                                                    gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccgcc                                                                        <  7:129999/144‑1 (MQ=255)                                                                                    gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccgcc                                                                        >  8:129999/1‑144 (MQ=255)                                                                                    gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                   >  4:112858/1‑149 (MQ=255)                                                                                              ggTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAc                                                         >  4:9645/1‑149 (MQ=255)                                                                                               gTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGt                                                                                  <  1:305700/123‑1 (MQ=255)                                                                                               gTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGt                                                                                  >  2:305700/1‑123 (MQ=255)                                                                                                       cgcCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGaa                                                <  7:45357/149‑1 (MQ=255)                                                                                                       cgcCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGaa                                                <  3:112858/149‑1 (MQ=255)                                                                                                              tGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcg                                         <  3:119187/149‑1 (MQ=255)                                                                                                                 cTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTGGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                                      >  1:225980/1‑149 (MQ=255)                                                                                                                        gCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGcc                                                                  <  6:195942/115‑1 (MQ=255)                                                                                                                        gCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGcc                                                                  >  5:195942/1‑115 (MQ=255)                                                                                                                        gCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTg                                 <  6:260768/148‑1 (MQ=255)                                                                                                                          aC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGa                                                                                    <  3:35946/95‑1 (MQ=255)                                                                                                                          aC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGa                                                                                    >  4:35946/1‑95 (MQ=255)                                                                                                                          aC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                                >  3:94217/1‑147 (MQ=255)                                                                                                                           c‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAg                             >  4:125691/1‑149 (MQ=255)                                                                                                                                 ttCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCt                           >  2:173467/1‑147 (MQ=255)                                                                                                                                 ttCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGAAGTTGGTCATCACCACGCGGAAGATTTCCATCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCa                         <  8:298716/149‑1 (MQ=255)                                                                                                                                   ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGc                       >  2:32470/1‑149 (MQ=255)                                                                                                                                    cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGaagctaagct                      >  7:109879/1‑149 (MQ=255)                                                                                                                                    cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                      >  2:14835/1‑149 (MQ=255)                                                                                                                                    cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                      <  2:48880/149‑1 (MQ=255)                                                                                                                                    cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                      >  3:135699/1‑149 (MQ=255)                                                                                                                                       gCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTgg                   >  8:216038/1‑149 (MQ=255)                                                                                                                                       gCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTgg                   <  1:173467/149‑1 (MQ=255)                                                                                                                                         cgacGACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTgg                   >  6:180145/4‑147 (MQ=255)                                                                                                                                         cgacGACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTgg                   <  5:180145/144‑1 (MQ=255)                                                                                                                                           ccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa                >  8:81842/1‑148 (MQ=255)                                                                                                                                             gACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGt                                                                     <  6:203049/93‑1 (MQ=255)                                                                                                                                             gACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGt                                                                     >  5:203049/1‑93 (MQ=255)                                                                                                                                                   caGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAgcgc        >  7:75487/1‑148 (MQ=255)                                                                                                                                                      gTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGcgcg       <  1:285486/146‑1 (MQ=255)                                                                                                                                                      gTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGcgcg       <  1:280995/146‑1 (MQ=255)                                                                                                                                                      gTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGcgcg       >  2:280995/1‑146 (MQ=255)                                                                                                                                                      gTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGcgcg       >  2:285486/1‑146 (MQ=255)                                                                                                                                                        gCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTg  >  4:67826/1‑149 (MQ=255)                                                                                                                                                        |                                                                                                                                                     GCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTG‑GCAC‑‑TTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTG  >  NC_002947/464174‑464470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑