Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,666,371 C→T 5.9% P129S (CCC→TCC)  nhaP → NhaP‑type Na+(K+)/H+ antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,666,3710CT 5.9% 207.4 / 2.1 68P129S (CCC→TCC) nhaPNhaP‑type Na+(K+)/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base C (27/37);  new base T (2/2);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TTTGCCGGCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCATCGTCGGCGAGTCACTGTTCAACGACGGCA  >  NC_002947/5666224‑5666509
                                                                                                                                                   |                                                                                                                                          
tttGCCGGCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGcc                                                                                                                                           >  4:261582/1‑149 (MQ=255)
     cGGCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGa                                                                                                                                      >  8:65194/1‑149 (MQ=255)
     cGGCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGa                                                                                                                                      <  3:261582/149‑1 (MQ=255)
      ggCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGAt                                                                                                                                     <  5:17775/149‑1 (MQ=255)
      ggCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGAt                                                                                                                                     >  7:7379/1‑149 (MQ=255)
            cTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTActg                                                                                                                               >  5:303142/1‑149 (MQ=255)
              gCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTAc                                                                                                                                 >  8:174993/1‑145 (MQ=255)
              gCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTAc                                                                                                                                 <  7:174993/145‑1 (MQ=255)
               cACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTActgcc                                                                                                                             <  6:303142/148‑1 (MQ=255)
               cACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGACGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTGATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTActgcc                                                                                                                             <  8:303140/148‑1 (MQ=255)
                aCGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTActgcctg                                                                                                                           <  3:280487/149‑1 (MQ=255)
                  gTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCctgct                                                                                                                         >  5:49533/1‑149 (MQ=255)
                  gTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCctgct                                                                                                                         >  6:998/1‑149 (MQ=255)
                     aaCCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGtt                                                                                                                      <  3:256542/149‑1 (MQ=255)
                       ccTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCg                                                                                                                    <  8:111002/149‑1 (MQ=255)
                       ccTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCg                                                                                                                    <  8:40508/149‑1 (MQ=255)
                       ccTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCCACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCg                                                                                                                    <  6:165684/149‑1 (MQ=255)
                           aGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGt                                                                                                                  <  8:216932/147‑1 (MQ=255)
                              gACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACt                                                                                                             >  2:236422/1‑149 (MQ=255)
                               aCCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTg                                                                                                            <  7:65194/149‑1 (MQ=255)
                                   gcgcAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCCCTGAtct                                                                                                        >  7:116582/1‑149 (MQ=255)
                                   gcgcAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGAtct                                                                                                        >  5:136277/1‑149 (MQ=255)
                                    cgccgcTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGCTCGCCACCGTGGTCAGCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGAtctc                                                                                                       <  3:185416/149‑1 (MQ=255)
                                      cAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCac                                                                                                     <  5:232540/149‑1 (MQ=255)
                                          tACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCaccgac                                                                                                 >  2:101894/1‑149 (MQ=255)
                                                       tCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGcc                                                                                    <  6:49533/149‑1 (MQ=255)
                                                          gCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGTCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGt                                                                                  >  5:314642/1‑148 (MQ=255)
                                                          gCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTAGCCCTGTTCGGCTGGCAGGTGTCCCTGATCTACTGCCTGCTGTTAGGTGCACTGATGTAACCTCAAGACCCGAATCCCGt                                                                                  >  3:189815/1‑148 (MQ=255)
                                                               ctgGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCtt                                                                              <  1:236422/147‑1 (MQ=255)
                                                               ctgGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACAGACCCGATTGCCGTGCTTgg                                                                            >  4:267600/1‑149 (MQ=255)
                                                                    tACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGgcgcgc                                                                       >  4:114410/1‑149 (MQ=255)
                                                                    tACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGgcgcgc                                                                       >  3:199994/1‑149 (MQ=255)
                                                                      ccTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTg                                                                     <  7:87363/149‑1 (MQ=255)
                                                                       cTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGc                                                                    >  3:291065/1‑149 (MQ=255)
                                                                             cGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCg                                                                                                                    >  4:176744/1‑95 (MQ=255)
                                                                             cGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCg                                                                                                                    <  3:176744/95‑1 (MQ=255)
                                                                                gCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTa                                                                 <  8:104628/143‑1 (MQ=255)
                                                                                gCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTa                                                                 >  7:104628/1‑143 (MQ=255)
                                                                                 cTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCaa                                                          <  4:110445/149‑1 (MQ=255)
                                                                                   gATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGc                                                             <  8:96078/144‑1 (MQ=255)
                                                                                   gATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGc                                                             <  8:91614/144‑1 (MQ=255)
                                                                                   gATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGc                                                             >  7:96078/1‑144 (MQ=255)
                                                                                   gATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGc                                                             >  7:91614/1‑144 (MQ=255)
                                                                                    aTCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATgc                                                       <  4:199994/149‑1 (MQ=255)
                                                                                       gCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATgcgc                                                     <  1:16309/148‑1 (MQ=255)
                                                                                       gCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATgcgc                                                     <  1:27074/148‑1 (MQ=255)
                                                                                       gCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGcc                                                    >  3:83128/1‑149 (MQ=255)
                                                                                       gCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGcc                                                    <  6:5433/149‑1 (MQ=255)
                                                                                        ccACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGccc                                                   <  4:291065/149‑1 (MQ=255)
                                                                                                 tCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCt                                           >  4:261103/1‑148 (MQ=255)
                                                                                                    tCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTaa                                        >  7:28991/1‑148 (MQ=255)
                                                                                                      ggattccTGGCTCATTGGGTGTTTGCCCTGTTCGGCTGGCAGTTGTCCCTGATCTTCTGGCTGCTGTTCGGTGCACTGATATCACCGACCGACCCGATTGCCGTGCTTGTCGCGCTTCGTACTTCCAATGCGCCCAAACCGCTTaaaa                                      <  4:189815/143‑1 (MQ=255)
                                                                                                      ggCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGTCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTaaaa                                      <  6:314642/148‑1 (MQ=255)
                                                                                                      ggCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTaaaa                                      <  8:7379/148‑1 (MQ=255)
                                                                                                       gCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAacc                                    <  4:83128/149‑1 (MQ=255)
                                                                                                          aCCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGc                                                             <  4:127995/121‑1 (MQ=255)
                                                                                                          aCCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGc                                                             >  3:127995/1‑121 (MQ=255)
                                                                                                             tGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCAtc                              >  6:133838/1‑149 (MQ=255)
                                                                                                               gcgcATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCAGTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCAtcgt                            >  2:299735/1‑149 (MQ=255)
                                                                                                               gcgcATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCAtcgt                            <  5:998/149‑1 (MQ=255)
                                                                                                               gcgcATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCAtc                              <  3:261103/147‑1 (MQ=255)
                                                                                                                 gcATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCATTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCAtcgtcg                          <  1:299735/149‑1 (MQ=255)
                                                                                                                 gcATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCAtcgtcg                          <  6:136277/149‑1 (MQ=255)
                                                                                                                   aTTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGc                                                                    <  7:66196/105‑1 (MQ=255)
                                                                                                                   aTTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTAACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGc                                                                    >  8:66196/1‑105 (MQ=255)
                                                                                                                                       ggCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCATCGTCGGCGAGTCACTGTTCAacgacg     <  3:123649/148‑1 (MQ=255)
                                                                                                                                        gCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTaa                                        <  2:3300/112‑1 (MQ=255)
                                                                                                                                        gCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTaa                                        >  1:3300/1‑112 (MQ=255)
                                                                                                                                         cTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCATCGTCGGCGAGTCACTGTTCAACGacggca  >  4:301295/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
TTTGCCGGCGCCCTGCACGTCAACCTCAGCGACCTGCGCAGCTACCGCTGGCCGATCGGCCTGCTGGCTACCTTGGGCGTGCTGATCGCCACCGTGGTCATCGGCTACCTGGCGCATTGGGTGTTTGCCCTGTTCGGCTGGCAGGTGCCCCTGATCTACTGCCTGCTGTTCGGTGCACTGATCTCACCGACCGACCCGATTGCCGTGCTTGGCGCGCTGCGTACTGCCAATGCGCCCAAACCGCTTAAAACCACCATCGTCGGCGAGTCACTGTTCAACGACGGCA  >  NC_002947/5666224‑5666509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑