Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,672,362 G→C 8.5% A204A (GCG→GCC)  PP_4979 → substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,672,3620GC 8.5% 132.1 / 2.6 45A204A (GCG→GCC) PP_4979substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base C (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTACTCAAGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTT  >  NC_002947/5672216‑5672509
                                                                                                                                                  |                                                                                                                                                   
ccTACTCAAGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCa                                                                                                                                                   <  5:93188/149‑1 (MQ=255)
       aagaagCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCACGCGCAGTACCTg                                                                                                                                            >  6:277109/1‑149 (MQ=255)
       aagaagCCAACTTCGGCAAGCTGATACTGGGCCGCATAGACCTGGTGATCACCGACCGCAGGTTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAAGAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTg                                                                                                                                            <  1:176740/149‑1 (MQ=255)
           agCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGc                                                                                                                                         <  6:310327/148‑1 (MQ=255)
             ccAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTg                                                                                                                                      >  7:225849/1‑149 (MQ=255)
             ccAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTg                                                                                                                                      >  3:271015/1‑149 (MQ=255)
              cAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTgg                                                                                                                                     >  3:280961/1‑149 (MQ=255)
                aCTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcg                                                                                                                                   <  4:280961/149‑3 (MQ=255)
                         aGCTGATACTGGGCCGCTTCGACCTGGTGATCAGCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAATAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcggctg                                                                                                                               <  6:16735/144‑7 (MQ=255)
                         aGCTGATACTGGGCCGCATCGACCTCGTGATCACCGACCGCAGGGTGGGGCATTACATGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcggctg                                                                                                                               >  5:16735/1‑138 (MQ=255)
                           cttaTACTGGGCCGCATCGACCTGGTGCTCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTgg                                                                                                                        <  7:284966/146‑1 (MQ=255)
                             gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGt                                                                                                                                    <  2:240345/135‑1 (MQ=255)
                             gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGt                                                                                                                                    >  1:240345/1‑135 (MQ=255)
                                aCTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGgcgc                                                                                                                    >  8:125088/1‑148 (MQ=255)
                                  tGGGCCGCATCGACCTCGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGACGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAg                                                                                                                 >  2:15960/1‑149 (MQ=255)
                                         cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                          >  8:177129/1‑149 (MQ=255)
                                         cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                          <  7:177129/149‑1 (MQ=255)
                                                   gTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGcc                                                                                                  <  7:125088/147‑1 (MQ=255)
                                                      aTCCCCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGc                                                                                             <  5:215251/149‑1 (MQ=255)
                                                        cACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGcc                                                                                            >  2:241412/1‑148 (MQ=255)
                                                         accgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                           >  7:283929/1‑148 (MQ=255)
                                                           cgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAag                                                                                        <  6:222731/149‑1 (MQ=255)
                                                             accgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                  <  2:251525/121‑1 (MQ=255)
                                                             accgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                  >  1:251525/1‑121 (MQ=255)
                                                                gCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTg                                                                                   <  2:108053/149‑1 (MQ=255)
                                                                  aGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTg                                                                                   >  8:35534/1‑147 (MQ=255)
                                                                       ggggCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                           <  5:231990/134‑1 (MQ=255)
                                                                       ggggCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                           >  6:231990/1‑134 (MQ=255)
                                                                          gCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTc                                                                          >  7:293729/1‑148 (MQ=255)
                                                                            aTTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCaat                                                                       <  1:15960/149‑2 (MQ=255)
                                                                              tACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAg                                                                       >  5:190033/1‑147 (MQ=255)
                                                                                    cTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCACGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAg                                                                 <  5:277109/147‑1 (MQ=255)
                                                                                    cTCCGCCAGCTCGGCCTGCAACAGCAAGTAGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTAACTGGCCCTGGTGCGAAAGCCTGGTCGCGAGGCACTGTCCGTGCCCATTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGcc                                                               >  8:195516/1‑149 (MQ=255)
                                                                                        gCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGcc                                                           >  5:303651/1‑149 (MQ=255)
                                                                                         ccAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCt                                                          >  7:95415/1‑149 (MQ=255)
                                                                                            gCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATg                                                       <  4:271015/149‑1 (MQ=255)
                                                                                                        aCAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGc                                            >  8:187037/1‑148 (MQ=255)
                                                                                                               gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCttt                                                                                               <  2:153838/90‑1 (MQ=255)
                                                                                                               gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTta                                    <  8:95415/149‑1 (MQ=255)
                                                                                                               gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTta                                    <  6:303651/149‑1 (MQ=255)
                                                                                                                           acggtGGTAATCAGCCGGCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGacac                        <  8:283929/145‑1 (MQ=255)
                                                                                                                                 gTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTg                      >  1:124227/1‑145 (MQ=255)
                                                                                                                                 gTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTg                      <  2:124227/145‑1 (MQ=255)
                                                                                                                                         ccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCa          <  7:35534/149‑1 (MQ=255)
                                                                                                                                               ggCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAAcc                             <  6:136742/124‑1 (MQ=255)
                                                                                                                                                 cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt  >  7:51941/1‑149 (MQ=255)
                                                                                                                                                 cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt  <  6:190033/149‑1 (MQ=255)
                                                                                                                                                  gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGt   >  8:160362/1‑147 (MQ=255)
                                                                                                                                                  gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGt   <  7:160362/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCTACTCAAGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTT  >  NC_002947/5672216‑5672509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑