Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,672,366 T→G 7.9% Y206D (TAC→GAC) ‡ PP_4979 → substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,672,3660TG 7.9% 151.4 / 2.2 49Y206D (TAC→GAC) ‡PP_4979substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base G (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAG  >  NC_002947/5672223‑5672512
                                                                                                                                               |                                                                                                                                                   
aagaagCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCACGCGCAGTACCTg                                                                                                                                                >  6:277109/1‑149 (MQ=255)
aagaagCCAACTTCGGCAAGCTGATACTGGGCCGCATAGACCTGGTGATCACCGACCGCAGGTTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAAGAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTg                                                                                                                                                <  1:176740/149‑1 (MQ=255)
    agCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGc                                                                                                                                             <  6:310327/148‑1 (MQ=255)
      ccAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTg                                                                                                                                          >  7:225849/1‑149 (MQ=255)
      ccAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTg                                                                                                                                          >  3:271015/1‑149 (MQ=255)
       cAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTgg                                                                                                                                         >  3:280961/1‑149 (MQ=255)
         aCTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcg                                                                                                                                       <  4:280961/149‑3 (MQ=255)
                  aGCTGATACTGGGCCGCTTCGACCTGGTGATCAGCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAATAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcggctg                                                                                                                                   <  6:16735/144‑7 (MQ=255)
                  aGCTGATACTGGGCCGCATCGACCTCGTGATCACCGACCGCAGGGTGGGGCATTACATGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcggctg                                                                                                                                   >  5:16735/1‑138 (MQ=255)
                    cttaTACTGGGCCGCATCGACCTGGTGCTCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTgg                                                                                                                            <  7:284966/146‑1 (MQ=255)
                      gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGt                                                                                                                                        >  1:240345/1‑135 (MQ=255)
                      gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGt                                                                                                                                        <  2:240345/135‑1 (MQ=255)
                         aCTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGgcgc                                                                                                                        >  8:125088/1‑148 (MQ=255)
                           tGGGCCGCATCGACCTCGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGACGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAg                                                                                                                     >  2:15960/1‑149 (MQ=255)
                                  cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                              >  8:177129/1‑149 (MQ=255)
                                  cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                              <  7:177129/149‑1 (MQ=255)
                                            gTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGcc                                                                                                      <  7:125088/147‑1 (MQ=255)
                                               aTCCCCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGc                                                                                                 <  5:215251/149‑1 (MQ=255)
                                                 cACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGcc                                                                                                >  2:241412/1‑148 (MQ=255)
                                                  accgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                               >  7:283929/1‑148 (MQ=255)
                                                    cgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAag                                                                                            <  6:222731/149‑1 (MQ=255)
                                                      accgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                      >  1:251525/1‑121 (MQ=255)
                                                      accgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                      <  2:251525/121‑1 (MQ=255)
                                                         gCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTg                                                                                       <  2:108053/149‑1 (MQ=255)
                                                           aGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTg                                                                                       >  8:35534/1‑147 (MQ=255)
                                                                ggggCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                               <  5:231990/134‑1 (MQ=255)
                                                                ggggCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                               >  6:231990/1‑134 (MQ=255)
                                                                   gCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTc                                                                              >  7:293729/1‑148 (MQ=255)
                                                                     aTTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCaat                                                                           <  1:15960/149‑2 (MQ=255)
                                                                       tACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAg                                                                           >  5:190033/1‑147 (MQ=255)
                                                                             cTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCACGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAg                                                                     <  5:277109/147‑1 (MQ=255)
                                                                             cTCCGCCAGCTCGGCCTGCAACAGCAAGTAGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTAACTGGCCCTGGTGCGAAAGCCTGGTCGCGAGGCACTGTCCGTGCCCATTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGcc                                                                   >  8:195516/1‑149 (MQ=255)
                                                                                 gCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGcc                                                               >  5:303651/1‑149 (MQ=255)
                                                                                  ccAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCt                                                              >  7:95415/1‑149 (MQ=255)
                                                                                     gCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATg                                                           <  4:271015/149‑1 (MQ=255)
                                                                                                 aCAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGc                                               >  8:187037/1‑148 (MQ=255)
                                                                                                        gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCttt                                                                                                   <  2:153838/90‑1 (MQ=255)
                                                                                                        gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTta                                       <  8:95415/149‑1 (MQ=255)
                                                                                                        gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTta                                       <  6:303651/149‑1 (MQ=255)
                                                                                                                    acggtGGTAATCAGCCGGCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGacac                           <  8:283929/145‑1 (MQ=255)
                                                                                                                          gTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTg                         >  1:124227/1‑145 (MQ=255)
                                                                                                                          gTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTg                         <  2:124227/145‑1 (MQ=255)
                                                                                                                                  ccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCa             <  7:35534/149‑1 (MQ=255)
                                                                                                                                        ggCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAAcc                                <  6:136742/124‑1 (MQ=255)
                                                                                                                                          cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt     >  7:51941/1‑149 (MQ=255)
                                                                                                                                          cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt     <  6:190033/149‑1 (MQ=255)
                                                                                                                                           gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGt      >  8:160362/1‑147 (MQ=255)
                                                                                                                                           gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGt      <  7:160362/147‑1 (MQ=255)
                                                                                                                                              gTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAATAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATTCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag  <  1:241412/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
AAGAAGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAG  >  NC_002947/5672223‑5672512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑