Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,672,372 G→C 7.4% G208R (GGC→CGC)  PP_4979 → substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,672,3720GC 7.4% 159.4 / 2.6 51G208R (GGC→CGC) PP_4979substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGG  >  NC_002947/5672227‑5672519
                                                                                                                                                 |                                                                                                                                                    
agCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGc                                                                                                                                                    <  6:310327/148‑1 (MQ=255)
  ccAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTg                                                                                                                                                 >  7:225849/1‑149 (MQ=255)
  ccAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTg                                                                                                                                                 >  3:271015/1‑149 (MQ=255)
   cAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTgg                                                                                                                                                >  3:280961/1‑149 (MQ=255)
     aCTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcg                                                                                                                                              <  4:280961/149‑3 (MQ=255)
              aGCTGATACTGGGCCGCTTCGACCTGGTGATCAGCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAATAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcggctg                                                                                                                                          <  6:16735/144‑7 (MQ=255)
              aGCTGATACTGGGCCGCATCGACCTCGTGATCACCGACCGCAGGGTGGGGCATTACATGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCCCAGGTACTGCGCCTggcggctg                                                                                                                                          >  5:16735/1‑138 (MQ=255)
                cttaTACTGGGCCGCATCGACCTGGTGCTCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTgg                                                                                                                                   <  7:284966/146‑1 (MQ=255)
                  gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGt                                                                                                                                               >  1:240345/1‑135 (MQ=255)
                  gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGt                                                                                                                                               <  2:240345/135‑1 (MQ=255)
                     aCTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGgcgc                                                                                                                               >  8:125088/1‑148 (MQ=255)
                       tGGGCCGCATCGACCTCGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGACGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAg                                                                                                                            >  2:15960/1‑149 (MQ=255)
                              cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                                     >  8:177129/1‑149 (MQ=255)
                              cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                                     <  7:177129/149‑1 (MQ=255)
                                        gTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGcc                                                                                                             <  7:125088/147‑1 (MQ=255)
                                           aTCCCCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGc                                                                                                        <  5:215251/149‑1 (MQ=255)
                                             cACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGcc                                                                                                       >  2:241412/1‑148 (MQ=255)
                                              accgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                                      >  7:283929/1‑148 (MQ=255)
                                                cgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAag                                                                                                   <  6:222731/149‑1 (MQ=255)
                                                  accgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                             <  2:251525/121‑1 (MQ=255)
                                                  accgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGa                                                                                                                             >  1:251525/1‑121 (MQ=255)
                                                     gCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTg                                                                                              <  2:108053/149‑1 (MQ=255)
                                                       aGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTg                                                                                              >  8:35534/1‑147 (MQ=255)
                                                            ggggCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                                      <  5:231990/134‑1 (MQ=255)
                                                            ggggCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                                      >  6:231990/1‑134 (MQ=255)
                                                               gCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTc                                                                                     >  7:293729/1‑148 (MQ=255)
                                                                 aTTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCaat                                                                                  <  1:15960/149‑2 (MQ=255)
                                                                   tACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAg                                                                                  >  5:190033/1‑147 (MQ=255)
                                                                         cTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCACGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAg                                                                            <  5:277109/147‑1 (MQ=255)
                                                                         cTCCGCCAGCTCGGCCTGCAACAGCAAGTAGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTAACTGGCCCTGGTGCGAAAGCCTGGTCGCGAGGCACTGTCCGTGCCCATTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGcc                                                                          >  8:195516/1‑149 (MQ=255)
                                                                             gCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGcc                                                                      >  5:303651/1‑149 (MQ=255)
                                                                              ccAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCt                                                                     >  7:95415/1‑149 (MQ=255)
                                                                                 gCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATg                                                                  <  4:271015/149‑1 (MQ=255)
                                                                                             aCAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGc                                                      >  8:187037/1‑148 (MQ=255)
                                                                                                    gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCttt                                                                                                          <  2:153838/90‑1 (MQ=255)
                                                                                                    gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTta                                              <  8:95415/149‑1 (MQ=255)
                                                                                                    gTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTta                                              <  6:303651/149‑1 (MQ=255)
                                                                                                                acggtGGTAATCAGCCGGCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGacac                                  <  8:283929/145‑1 (MQ=255)
                                                                                                                      gTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTg                                >  1:124227/1‑145 (MQ=255)
                                                                                                                      gTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTg                                <  2:124227/145‑1 (MQ=255)
                                                                                                                              ccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCa                    <  7:35534/149‑1 (MQ=255)
                                                                                                                                    ggCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAAcc                                       <  6:136742/124‑1 (MQ=255)
                                                                                                                                      cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt            >  7:51941/1‑149 (MQ=255)
                                                                                                                                      cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt            <  6:190033/149‑1 (MQ=255)
                                                                                                                                       gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGt             >  8:160362/1‑147 (MQ=255)
                                                                                                                                       gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGt             <  7:160362/147‑1 (MQ=255)
                                                                                                                                          gTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAATAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATTCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag         <  1:241412/149‑1 (MQ=255)
                                                                                                                                             ccTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGagcag      >  7:227332/1‑148 (MQ=255)
                                                                                                                                             ccTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGagcag      >  8:63145/1‑148 (MQ=255)
                                                                                                                                              cTGGGCCTGGTGCGCAAGGCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGTAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAgcagca    <  7:195516/149‑1 (MQ=255)
                                                                                                                                               tGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGcagcag   <  8:227332/149‑1 (MQ=255)
                                                                                                                                                 ggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGGCGTTGagaatgttt  <  7:81304/148‑8 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCCAACTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGA‑TCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGG  >  NC_002947/5672227‑5672519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑