| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,758,996 | G→A | 7.3% | A27T (GCC→ACC) | trmL → | tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,758,996 | 0 | G | A | 7.3% | 153.7 / 2.6 | 55 | A27T (GCC→ACC) | trmL | tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL |
| Reads supporting (aligned to +/- strand): ref base G (26/24); new base A (2/2); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
AGCATCACCCGTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCTTGGCAA > NC_002947/5758849‑5759144 | aGCATCACCCGTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑Tgc‑gc < 1:313618/149‑1 (MQ=255) gCATCACCCGTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑Gcc < 3:141302/149‑1 (MQ=255) aCCCGTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGTGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAg > 1:160598/1‑149 (MQ=255) gTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGc > 1:128701/1‑149 (MQ=255) gTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGc < 1:287762/149‑1 (MQ=255) ggAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTgc < 6:148105/149‑1 (MQ=255) ttCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCCTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑Ca > 2:275613/1‑149 (MQ=255) gTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAAc < 3:82168/149‑1 (MQ=255) tCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAAcc < 4:248994/149‑1 (MQ=255) tCCGGGTCATGGGTATAATTCCCGTCATCTTCACGTTTGGATCCGTTCATGTTTCACGTCCTTCTTTTTGAACCAGTAATTCCGGCGTTGATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGaa < 1:253945/149‑1 (MQ=255) ccGTCATCTTCACGTTTGGAGCCGTGCATGGTTCACGTCATCCTTTTTCAACCTGAAATTCCGCCG‑‑AATAACGGCACCATCAATCGGCTA‑TGC‑TCCACCAGCGGCTG‑GCGCAGTCAGCTGATCGAACCCCTCAGCAACGAGCTTGATgt > 4:213427/1‑148 (MQ=255) tCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAgcg > 5:272323/1‑149 (MQ=255) ttCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGcc > 5:97008/1‑149 (MQ=255) tCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCt > 6:228138/1‑149 (MQ=255) tCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCt > 3:205202/1‑149 (MQ=255) cACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCAG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTg > 4:257717/1‑149 (MQ=255) aCGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTgc < 5:182792/149‑1 (MQ=255) gTTTGGAGCCGTGAATGTTACACGTAATCCTTTTTCAACCAGAAAATACGCCG‑‑AATACCGGCAACATCATTACTCTA‑TGA‑GCCAACAGCGGAAGCGCCCTg > 7:124995/1‑101 (MQ=255) tttGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑ACCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGcgcc > 8:180740/1‑149 (MQ=255) aGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATc > 4:136154/1‑113 (MQ=255) aGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATc < 3:136154/113‑1 (MQ=255) ccGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCAGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGAt > 8:263498/1‑126 (MQ=255) ccGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCAGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGAt < 7:263498/126‑1 (MQ=255) cATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTg < 3:257717/148‑1 (MQ=255) cATGTTTCACGTCATCCTATTTCAACCAGACATTCCCCCG‑‑AATACCGGCAACCTCATTCTTCTA‑TCC‑GCCAACAGCGGCTGCGCCCTG‑CTCCTGATCGAACCCATCAGCTTCCTACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTg < 6:272323/148‑1 (MQ=255) tttCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACt < 5:228138/148‑1 (MQ=255) tttCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTa > 6:192708/1‑149 (MQ=255) cATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCAGCGCCCTG‑CACCTGa > 7:196654/1‑83 (MQ=255) aTCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGAAGGGCTGGACTACCACGAGTa > 4:88252/1‑149 (MQ=255) aTCCTTTTTCAACCAGAAAATCCCCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTa < 4:241374/149‑1 (MQ=255) ccTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTAGTGC‑ACCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTAt > 1:237824/1‑149 (MQ=255) tttttCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAAGCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGc > 6:105408/1‑148 (MQ=255) tttttCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGc > 2:95093/1‑148 (MQ=255) tttCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGCGGCAAACAGCGGCTGCGCCATG‑CACATGATCGAACCAAACAGCTTCGACATGGATGACAAGCGCCTGCGCCGCGCAGGGATGGAATACAACGAGTATGCCa > 1:237833/1‑149 (MQ=255) caccccgAAATTCCGCCC‑‑ACTACCCCCAACATCCTGCGCCTA‑TGC‑GCCCCCCCCCGCCGCGCCCGC‑CCCCGGAGCGACCCCCTCAGCTTCGAACTGGCGCACACGCGCCTGCGCCGCGCAGGGCGGGACTACCCCGAGCATGCCCCGc < 6:97008/142‑1 (MQ=255) ccAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTc > 2:33405/1‑147 (MQ=255) aaTTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGcc < 1:95093/149‑1 (MQ=255) aTTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAg > 1:276054/1‑112 (MQ=255) aTTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAg < 2:276054/112‑1 (MQ=255) aaTACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCtt < 1:33405/149‑1 (MQ=255) cGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCg > 3:175760/1‑149 (MQ=255) ggCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCgg < 2:160598/149‑1 (MQ=255) gCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGa > 6:211619/1‑149 (MQ=255) gCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGa > 6:263169/1‑149 (MQ=255) cAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGa < 6:108542/111‑1 (MQ=255) cAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGa > 5:108542/1‑111 (MQ=255) atcatTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCt < 2:237833/149‑1 (MQ=255) catTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGACTg > 4:196926/1‑148 (MQ=255) cGTCTA‑TGC‑ACCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGaaa < 7:180740/148‑1 (MQ=255) gTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGc < 3:88252/149‑1 (MQ=255) tCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGcc < 1:275613/149‑1 (MQ=255) tCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGcc < 4:175760/149‑1 (MQ=255) a‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCTTg > 1:36047/1‑149 (MQ=255) tGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCtt < 5:263169/147‑1 (MQ=255) tGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCTTgg > 6:129413/1‑149 (MQ=255) tGC‑ACCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCTTgg < 2:237824/149‑1 (MQ=255) gcCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCTTGGCaa > 3:314468/1‑149 (MQ=255) | AGCATCACCCGTGGGAAGGCCTTCGAACGCACGTCCGGTTCAGGGGTAGAATGCCCGTCATCTTCACGTTTGGAGCCGTGCATGTTTCACGTCATCCTTTTTCAACCAGAAATTCCGCCG‑‑AATACCGGCAACATCATTCGTCTA‑TGC‑GCCAACAGCGGCTGCGCCCTG‑CACCTGATCGAACCCATCAGCTTCGAACTGGATGACAAGCGCCTGCGCCGCGCAGGGCTGGACTACCACGAGTATGCCACGCTCAAGCGCCACGAGAGCTTGGCCGGATGCCTGGAAAGCCTTGGCAA > NC_002947/5758849‑5759144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |