Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,780,693 T→A 7.3% S333T (TCG→ACG) ‡ cvrA → K(+)/H(+) antiporter NhaP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,780,6930TA 7.3% 191.5 / 5.4 69S333T (TCG→ACG) ‡cvrAK(+)/H(+) antiporter NhaP
Reads supporting (aligned to +/- strand):  ref base T (26/38);  new base A (3/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

CCTGCACATGCTCGACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTT  >  NC_002947/5780545‑5780838
                                                                                                                                                     |                                                                                                                                                  
ccTGCACATGCTCGACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGt                                                                                                                                                    >  2:248062/1‑149 (MQ=255)
    cacaTGCTCGACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCggt                                                                                                                                                <  1:248062/149‑1 (MQ=255)
    cacaTGCTCGACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCgg                                                                                                                                                 <  1:260169/148‑1 (MQ=255)
           tCGACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTcg‑cg                                                                                                                                        >  5:154054/1‑149 (MQ=255)
             gACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑Cgg                                                                                                                                       <  2:310746/148‑1 (MQ=255)
               cGGCATGGCCTGTCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGTGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGACCACCGACAGTGGCcg‑cgcc                                                                                                                                      <  3:234913/147‑3 (MQ=255)
                   aTGGCCTGGCTGGCCCAGATGGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATGGCCCTGCCGGCAGTGGGCCTGGCGCTCTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCctgct                                                                                                                                <  2:124166/149‑1 (MQ=255)
                   aTGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCctgct                                                                                                                                <  7:225074/149‑1 (MQ=255)
                       ccTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCt                                                                                                                            >  6:328999/1‑149 (MQ=255)
                           gctggcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTc                                                                                                                         >  5:129986/1‑148 (MQ=255)
                           agtatcccAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCa                                                                                                                        <  7:174355/144‑1 (MQ=255)
                            ctggcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCaa                                                                                                                       <  1:232075/149‑1 (MQ=255)
                             tggcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAg                                                                                                                      >  6:147285/1‑149 (MQ=255)
                             tggcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAg                                                                                                                      >  4:204888/1‑149 (MQ=255)
                             tggcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAg                                                                                                                      <  5:147285/149‑1 (MQ=255)
                               gcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAgg                                                                                                                     <  4:126950/148‑1 (MQ=255)
                               gcCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAgg                                                                                                                     <  8:208101/148‑1 (MQ=255)
                                 ccAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑Cgg                                                                                                                                       >  2:101184/1‑128 (MQ=255)
                                 ccAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCAGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑Cgg                                                                                                                                       <  1:101184/128‑1 (MQ=255)
                                     aTCGGCATGTTGCGGGTGCTTGGGTTGCTGGGCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGGGGCGCGGCCACTGTCGGTGGGCGTCGGCCCTGCTGCCTTTCAAGGCTTTcc                                                                                                               <  1:173510/149‑1 (MQ=255)
                                          cATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGcc                                                                                                         >  7:92069/1‑149 (MQ=255)
                                               tCCTGGTGCTGGGGTTGGTGGGCCGGCGGCAGTCCCTGTGGCCCATCCCCCTGCCGGCCCGGGGCGTGGGGCGGGGTTTTTTCCATGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTg                                                                                                      <  1:258083/148‑1 (MQ=255)
                                                 cTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGaaaa                                                                                                  <  3:204888/149‑1 (MQ=255)
                                                                cTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGt                                                                                    <  6:129986/148‑1 (MQ=255)
                                                                cTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGt                                                                                    <  8:92069/148‑1 (MQ=255)
                                                                cTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGt                                                                                    <  3:103855/148‑1 (MQ=255)
                                                                   gTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TTGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCACTGCTGCCTTTCAAGGCTTTCCAAGGCCGTGAAAAGGGCTTCATTTCGTGGGt                                                                                >  8:221744/1‑149 (MQ=255)
                                                                   gTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGt                                                                                >  1:281774/1‑149 (MQ=255)
                                                                       cgccgcACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGc                                                                            <  8:118389/149‑1 (MQ=255)
                                                                         ccgcACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCt                                                                          >  3:56915/1‑149 (MQ=255)
                                                                         ccgcACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCt                                                                          >  7:249394/1‑149 (MQ=255)
                                                                            cACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcg                                                                       <  8:249394/149‑1 (MQ=255)
                                                                                aCCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTgg                                                                                  <  8:266486/134‑1 (MQ=255)
                                                                                aCCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTgg                                                                                  >  7:266486/1‑134 (MQ=255)
                                                                                 ccTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcgcggcg                                                                  >  3:229916/1‑149 (MQ=255)
                                                                                 ccTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcgcggcg                                                                  <  5:282175/149‑1 (MQ=255)
                                                                                   tgctgcCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcgcggcgcg                                                                >  7:122878/1‑149 (MQ=255)
                                                                                        cccATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCtt                                                                                            >  8:245727/1‑116 (MQ=255)
                                                                                        cccATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCtt                                                                                            <  7:245727/116‑1 (MQ=255)
                                                                                                 cTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCt                                                  <  1:235651/149‑1 (MQ=255)
                                                                                                           tGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTc                                        >  6:199113/1‑149 (MQ=255)
                                                                                                           tGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTc                                        >  4:55853/1‑149 (MQ=255)
                                                                                                              gCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCg                                     <  4:56915/149‑1 (MQ=255)
                                                                                                                cTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCt                                   <  3:55853/149‑1 (MQ=255)
                                                                                                                        gtgGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGGCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCgg                           <  5:74112/149‑1 (MQ=255)
                                                                                                                         tgGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCg                            <  2:281774/147‑1 (MQ=255)
                                                                                                                           gatgatCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGcc                        >  7:2932/1‑149 (MQ=255)
                                                                                                                           gatgatCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGc                         <  5:199113/148‑1 (MQ=255)
                                                                                                                                tCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTc                                        >  1:979/1‑128 (MQ=255)
                                                                                                                                tCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTc                                        <  2:979/128‑1 (MQ=255)
                                                                                                                                    tggtggCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGAc                >  3:18542/1‑149 (MQ=255)
                                                                                                                                    tggtggCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGAc                >  5:200090/1‑149 (MQ=255)
                                                                                                                                      gtggCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGc              >  4:15728/1‑149 (MQ=255)
                                                                                                                                       ggccgcgACCACCGACAGTGGCCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGcc             >  8:242340/4‑149 (MQ=255)
                                                                                                                                        ggCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCg                                              >  2:244158/1‑115 (MQ=255)
                                                                                                                                        ggCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCg                                              <  1:244158/115‑1 (MQ=255)
                                                                                                                                         gcgcGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCa           <  6:200090/149‑1 (MQ=255)
                                                                                                                                         ccgcgACCACCGACAGTGGCCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGc                                    >  5:59993/2‑124 (MQ=255)
                                                                                                                                         ccgcgACCACCGACAGTGGCCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGc                                    <  6:59993/123‑1 (MQ=255)
                                                                                                                                          cgcgGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTGAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAg          <  5:328999/149‑1 (MQ=255)
                                                                                                                                          cgcgGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAg          <  3:76367/149‑1 (MQ=255)
                                                                                                                                           gcgGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGa                                                         <  3:184191/101‑1 (MQ=255)
                                                                                                                                           gcgGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGa                                                         >  4:184191/1‑101 (MQ=255)
                                                                                                                                           gcgGCCACTGTCGGTGGCCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGc                                    <  7:272160/122‑1 (MQ=255)
                                                                                                                                           gcgACCACCGACAGTGGCCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGc         >  6:87367/1‑149 (MQ=255)
                                                                                                                                              gCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGt      >  2:220255/1‑149 (MQ=255)
                                                                                                                                                 aCTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGttct   <  4:229916/149‑1 (MQ=255)
                                                                                                                                                  cTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGttctt  <  4:7734/149‑1 (MQ=255)
                                                                                                                                                   tGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTAc                                                            <  2:316850/90‑1 (MQ=255)
                                                                                                                                                   tGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTAc                                                            >  1:316850/1‑90 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCTGCACATGCTCGACGGCATGGCCTGGCTGGCCCAGATCGGCATGTTCCTGGTGCTGGGGTTGCTGGTCACGCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCC‑TGGTGGCGCGGCCACTGTCGGTGGTCG‑CGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTT  >  NC_002947/5780545‑5780838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑