Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,822,596 A→G 8.1% E42G (GAA→GGA)  rdgB → dITP/XTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,822,5960AG 8.1% 137.4 / 3.3 49E42G (GAA→GGA) rdgBdITP/XTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base G (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

CAGGAAGTGTTCCCAGGCGAATGATGAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCGGGCATCTACTCGGCGCGCTATGC  >  NC_002947/5822452‑5822743
                                                                                                                                                |                                                                                                                                                   
cAGGAAGTGTTCCCAGGCGAATGATGAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGACCTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGgaaga                                                                                                                                                  >  4:242954/1‑148 (MQ=255)
   gAAGTGTTCCCAGTCGAATGATGAATTTCCAGCAACTCGTTTTGGCCAGCCACAATGCCGGCAAACTTAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAAc                                                                                                                                               <  4:219091/148‑1 (MQ=255)
           cccAGGCGAATGATGAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTc                                                                                                                                      >  7:155611/1‑149 (MQ=255)
            ccAGGCGAATGATGAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCg                                                                                                                                     >  1:66356/1‑149 (MQ=255)
                  gAATGATGAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGTCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTc                                                                                                                               <  7:210492/149‑1 (MQ=255)
                    atgatgAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCg                                                                                                                              <  8:34563/148‑1 (MQ=255)
                        tgaATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAAc                                                                                                                         >  5:222435/1‑149 (MQ=255)
                        tgaATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAAc                                                                                                                         <  3:234939/149‑1 (MQ=255)
                        tgaATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAAc                                                                                                                         <  2:66356/149‑1 (MQ=255)
                           aTTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGc                                                                                                                                           >  2:291379/1‑128 (MQ=255)
                            tttCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCa                                                                                                                     >  6:309627/1‑149 (MQ=255)
                                cAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGttt                                                                                                                                  >  4:73961/1‑132 (MQ=255)
                                   cAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCaa                                                                                                              <  1:149902/149‑1 (MQ=255)
                                       tCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGgcg                                                                                                          >  7:92140/1‑149 (MQ=255)
                                           aTTGGCCAGCCACAATGCGGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGgcgcgc                                                                                                       >  3:122528/1‑148 (MQ=255)
                                                   gccaCAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACgccgcc                                                                                              <  2:242790/149‑1 (MQ=255)
                                                      acaATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGccgcccg                                                                                            >  4:120172/1‑148 (MQ=255)
                                                             cGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCg                                                                                    <  8:92140/149‑1 (MQ=255)
                                                                     tGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCg                                                                            >  7:28712/1‑149 (MQ=255)
                                                                          gAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCtt                                                                       <  5:309627/149‑1 (MQ=255)
                                                                              tCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGc                                                                                                                                           <  2:219715/77‑1 (MQ=255)
                                                                              tCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGc                                                                                                                                           >  1:219715/1‑77 (MQ=255)
                                                                              tCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGcc                                                                   >  3:90810/1‑149 (MQ=255)
                                                                                cAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGa                                                                 <  7:54200/149‑1 (MQ=255)
                                                                                   gCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGGAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCg                                                                                    <  7:82237/127‑1 (MQ=255)
                                                                                   gCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGGAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCg                                                                                    >  8:82237/1‑127 (MQ=255)
                                                                                   gCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGGAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCg                                                                                    >  6:280378/1‑127 (MQ=255)
                                                                                   gCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGGAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCg                                                                                    <  5:280378/127‑1 (MQ=255)
                                                                                   gCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGa                                                              >  1:158447/1‑149 (MQ=255)
                                                                                       tGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGActct                                                          <  1:170071/149‑1 (MQ=255)
                                                                                         cTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTgg                                                        <  8:155611/149‑1 (MQ=255)
                                                                                          tCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTg                                                         >  5:284704/1‑147 (MQ=255)
                                                                                          tCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTg                                                         <  6:284704/147‑1 (MQ=255)
                                                                                          tCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTg                                                         >  1:39197/1‑147 (MQ=255)
                                                                                          tCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTg                                                         <  2:39197/147‑1 (MQ=255)
                                                                                          tCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGa                                                       >  8:100358/1‑149 (MQ=255)
                                                                                                 ttCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCgg                                                >  3:11927/1‑149 (MQ=255)
                                                                                                   ccGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGt                                               <  7:100358/148‑1 (MQ=255)
                                                                                                     gTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGa                                            >  4:160942/1‑149 (MQ=255)
                                                                                                     gTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTGTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGa                                            <  3:242954/149‑1 (MQ=255)
                                                                                                           cTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCt                                      <  6:222435/149‑1 (MQ=255)
                                                                                                              cACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCg                                    <  8:28712/148‑1 (MQ=255)
                                                                                                                cTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTg                                 <  4:122528/149‑1 (MQ=255)
                                                                                                                cTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTg                                 <  4:90810/149‑1 (MQ=255)
                                                                                                                 tCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTgg                                >  3:41798/1‑149 (MQ=255)
                                                                                                                      tGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGgcgcgc                           >  7:195748/1‑149 (MQ=255)
                                                                                                                          gAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCggg                       >  8:298746/1‑149 (MQ=255)
                                                                                                                             ttCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCGGGCAt                    <  3:120172/149‑1 (MQ=255)
                                                                                                                                            ccGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCGAGTAGATGCCCGgcgcgcca     <  3:160942/149‑3 (MQ=255)
                                                                                                                                              ggAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCGGGCATCTACTCGGCGCGCTATg   >  7:155464/1‑149 (MQ=255)
                                                                                                                                               gaagaaACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCGGGCATCTACTCGGCGCGCTATGc  >  8:30926/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CAGGAAGTGTTCCCAGGCGAATGATGAATTTCCAGCAACTCGTATTGGCCAGCCACAATGCCGGCAAACTGAAGGAACTCCAGGCCATGCTCGGCCATTCCGTGCAGCTGCACTCGATTGGTGAGTTCAGCCAGGTGGAGCCGGAAGAAACCGGCCTGTCGTTTGTCGAGAACGCCATCCTCAAGGCGCGCAACGCCGCCCGCATTTCCGGCCTGCCGGCCTTGGCCGATGACTCTGGACTGGCGGTGGACTTCCTCGGTGGCGCGCCGGGCATCTACTCGGCGCGCTATGC  >  NC_002947/5822452‑5822743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑