Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,890,599 T→G 7.0% F752V (TTC→GTC)  PP_5164 → penicillin amidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,890,5990TG 7.0% 160.8 / 4.1 57F752V (TTC→GTC) PP_5164penicillin amidase family protein
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base G (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCCATGAAGTGCCGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGC  >  NC_002947/5890453‑5890746
                                                                                                                                                  |                                                                                                                                                   
cccATGAAGTGCCGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGG‑AGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTc                                                                                                                                                   <  2:219661/148‑1 (MQ=255)
cccATGAAGTGCCGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTCACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTc                                                                                                                                                   <  8:282236/149‑1 (MQ=255)
   aTGAAGTGCCGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGAc                                                                                                                                                >  3:175814/1‑149 (MQ=255)
           ccGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCAtt                                                                                                                                         <  4:79780/148‑1 (MQ=255)
            cGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTAc                                                                                                                                       >  4:107871/1‑149 (MQ=255)
              aacaacCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTAcgc                                                                                                                                     <  7:134571/149‑1 (MQ=255)
                    ctctCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCa                                                                                                                               <  4:263239/149‑1 (MQ=255)
                    ctctCGGCGAAGATCAGCTCTGCGCCATCGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGAc                                                                                                                                 >  1:182365/1‑147 (MQ=255)
                      ctcGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCaag                                                                                                                             >  5:216719/1‑147 (MQ=255)
                            gAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAgg                                                                                                                       <  8:269945/149‑1 (MQ=255)
                               gttCAGCTCTGGGCATTGGCGGGTGAGCTATTGGCCGTCGACGCGGAGGATGATCGACTTTGACGATGCCGGGCAGGCGCTGACCATCAACCCGGTCTGGCAGAGTGGCGTGCCGTTCTACCGGCATTACGCTGACCAGGCCTAGGGGt                                                                                                                    <  4:136424/147‑1 (MQ=255)
                                 tCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTAt                                                                                                                  <  3:311077/149‑1 (MQ=255)
                                          cgcCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGTGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAg                                                                                                            <  8:85294/146‑1 (MQ=255)
                                           gcCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAgggg                                                                                                         >  3:138662/1‑148 (MQ=255)
                                              aTGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAg                                                                                                      >  4:125334/1‑148 (MQ=255)
                                                  ccGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCa                                                                                                 <  4:138662/149‑1 (MQ=255)
                                                      tGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCgg                                                                                             <  3:107871/149‑1 (MQ=255)
                                                        aCCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAgggg                                                                                                                     <  6:94785/123‑1 (MQ=255)
                                                          cTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGgcg                                                                                          >  6:305617/1‑148 (MQ=255)
                                                               ggCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGAt                                                                                     <  5:305617/148‑1 (MQ=255)
                                                                gCCGGCGACGCGGGGGCTGATCGACTTAGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATgg                                                                                   <  4:175814/149‑1 (MQ=255)
                                                                  cGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATgggg                                                                                 <  7:230592/149‑1 (MQ=255)
                                                                           ggcggcTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCg                                                                        >  4:298028/1‑149 (MQ=255)
                                                                            gcggcTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCgg                                                                       >  2:172751/1‑149 (MQ=255)
                                                                              ggcTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGgcg                                                                     >  1:20935/1‑149 (MQ=255)
                                                                                        cTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCt                                                           >  7:7520/1‑149 (MQ=255)
                                                                                         tttGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCt                                                           >  8:70375/1‑148 (MQ=255)
                                                                                            gCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACg                                                       >  3:31290/1‑149 (MQ=255)
                                                                                            gCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACg                                                       >  7:265887/1‑149 (MQ=255)
                                                                                                    cGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACgg                                                      >  2:284021/1‑142 (MQ=255)
                                                                                                    cGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACgg                                                      <  1:284021/142‑1 (MQ=255)
                                                                                                      ggCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTAccc                                             <  4:31290/149‑1 (MQ=255)
                                                                                                           gcgcTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGaa                                        <  2:182365/149‑1 (MQ=255)
                                                                                                             gcTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTACGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAg                                       >  2:292629/1‑148 (MQ=255)
                                                                                                                 aCCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTaa                                    <  3:298028/147‑1 (MQ=255)
                                                                                                                   cATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTaa                                    <  6:30791/145‑1 (MQ=255)
                                                                                                                   cATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTaa                                    >  5:30791/1‑145 (MQ=255)
                                                                                                                    aTCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGaa                                        <  4:2071/140‑1 (MQ=255)
                                                                                                                    aTCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGaa                                        >  3:2071/1‑140 (MQ=255)
                                                                                                                     tCAACCAGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCAATACGCCGACCAGTCCGAGGGGGAAGTGCAGGGGCAGTATCAGCGGGGGCAGATGGGGGTGGTTACGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGgct                              >  1:283781/1‑149 (MQ=255)
                                                                                                                       aaCCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTgg                                                  <  5:60105/127‑1 (MQ=255)
                                                                                                                       aaCCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTgg                                                  >  6:60105/1‑127 (MQ=255)
                                                                                                                            ggTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGc                       >  1:160036/1‑149 (MQ=255)
                                                                                                                             gTCGGGCAGAGGGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCg                      <  8:7520/149‑1 (MQ=255)
                                                                                                                                  gCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATggggg                                                                                >  6:27320/1‑86 (MQ=255)
                                                                                                                                  gCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATggggg                                                                                <  5:27320/86‑1 (MQ=255)
                                                                                                                                   cAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCt                >  4:89826/1‑149 (MQ=255)
                                                                                                                                     gagTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTc              >  7:271529/1‑149 (MQ=255)
                                                                                                                                       gcgtaatGCCGGTCGAACGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTaa                                    <  6:87661/119‑1 (MQ=255)
                                                                                                                                       gcgtaatGCCGGTCGAACGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTaa                                    >  5:87661/7‑125 (MQ=255)
                                                                                                                                        tGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATAAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCg             >  8:227899/1‑147 (MQ=255)
                                                                                                                                         gtaatGCCGGTCGAACGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGgc                               >  4:117023/5‑128 (MQ=255)
                                                                                                                                         gtaatGCCGGTCGAACGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGgc                               <  3:117023/124‑1 (MQ=255)
                                                                                                                                                cGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAgg   <  3:89826/149‑1 (MQ=255)
                                                                                                                                                 gTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAg    <  7:228894/147‑1 (MQ=255)
                                                                                                                                                 gTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAg    >  8:228894/1‑147 (MQ=255)
                                                                                                                                                 gTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGc  <  6:216719/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCCATGAAGTGCCGAACAACCTCTCGGCGAAGATCAGCTCTGCGCCATGGCCGGTGACCTATGGGCCGTCGACGCGGCGGCTGATCGACTTTGCCGATGCCGGGCAGGCGCTGACCATCAACCCGGTCGGGCAGAGTGGCGTGCCGTTCGACCGGCATTACGCGGACCAGGCCGAGGGGTATGTGCAGGGGCAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGC  >  NC_002947/5890453‑5890746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑