Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,890,791 A→C 6.2% intergenic (+79/+41) PP_5164 → / ← plpB penicillin amidase family protein/NLPA lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,890,7910AC 6.2% 200.3 / 4.7 64intergenic (+79/+41)PP_5164/plpBpenicillin amidase family protein/NLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base C (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

AGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTT  >  NC_002947/5890645‑5890936
                                                                                                                                                   |                                                                                                                                                 
aGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                 >  2:310455/1‑149 (MQ=255)
     cAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACCCCGACACAAGGTGTc                                                                                                                                            >  7:189767/1‑149 (MQ=255)
       gCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                          <  4:306602/149‑1 (MQ=255)
        cGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                          <  1:159937/148‑1 (MQ=255)
        cGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                          >  2:159937/1‑148 (MQ=255)
             gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTgg                                                                                                                                    <  1:202230/149‑1 (MQ=255)
             gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTgg                                                                                                                                    <  4:255952/149‑1 (MQ=255)
             gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTgg                                                                                                                                    <  1:151559/149‑1 (MQ=255)
                    ggggTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                             <  3:82412/149‑1 (MQ=255)
                     ggggGATTCCGGCGCAAACTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGTCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATc                                                                                                                            <  8:265887/149‑1 (MQ=255)
                            tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCa                                                                                                                           <  6:72447/143‑1 (MQ=255)
                            tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCa                                                                                                                           >  5:72447/1‑143 (MQ=255)
                            tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGtt                                                                                                                     >  6:107300/1‑149 (MQ=255)
                              cGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGc                                                                                                                   >  5:306665/1‑149 (MQ=255)
                                 cgcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAg                                                                                                                >  2:170427/1‑149 (MQ=255)
                                  gcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGc                                                                                                               <  1:292629/149‑1 (MQ=255)
                                   cAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                             <  5:242301/134‑1 (MQ=255)
                                   cAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                             >  6:242301/1‑134 (MQ=255)
                                         aCCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATc                                                                                                         <  2:20935/148‑1 (MQ=255)
                                          ccTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAAt                                                                                                          >  2:221339/1‑146 (MQ=255)
                                          ccTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCa                                                                                                        <  6:127329/148‑1 (MQ=255)
                                                       aCCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTa                                                                                          >  6:111379/1‑149 (MQ=255)
                                                        cccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTa                                                                                          <  6:306665/148‑1 (MQ=255)
                                                                gTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCtt                                                                                 >  6:236806/1‑149 (MQ=255)
                                                                   agagGCTGCTTTGCGGTCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTg                                                                                <  6:270692/147‑1 (MQ=255)
                                                                   agagGCTGCTTTGCGGACGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTg                                                                                >  5:270692/1‑147 (MQ=255)
                                                                           ctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCAGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTg                                                                      >  1:61569/1‑149 (MQ=255)
                                                                            tttGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                     >  4:154924/1‑149 (MQ=255)
                                                                                  gCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatg                                                                >  8:273966/1‑148 (MQ=255)
                                                                                   ccGCTTCGCAGCAAAAGGCTTCTACGGTGCGTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatg                                                                >  1:29493/1‑148 (MQ=255)
                                                                                    cGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGaa                                                                                                            >  4:22999/1‑102 (MQ=255)
                                                                                    cGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGaa                                                                                                            <  3:22999/102‑1 (MQ=255)
                                                                                    cGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatgaat                                                             >  3:11089/1‑149 (MQ=255)
                                                                                         cGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGcc                                                          <  8:136557/147‑1 (MQ=255)
                                                                                          gcagcaAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                        <  1:221339/148‑1 (MQ=255)
                                                                                          gcagcaAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                        <  8:189767/148‑1 (MQ=255)
                                                                                              caAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACtt                                                   <  2:29493/149‑1 (MQ=255)
                                                                                                 aaGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCg                                                >  3:284008/1‑149 (MQ=255)
                                                                                                 aaGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAAAGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGAATTCCg                                                >  4:198821/1‑149 (MQ=255)
                                                                                                   ggCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                              <  5:111379/149‑1 (MQ=255)
                                                                                                   ggCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                              <  7:273966/149‑1 (MQ=255)
                                                                                                          aCGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGa                                                      >  3:267477/1‑134 (MQ=255)
                                                                                                          aCGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGa                                                      <  4:267477/134‑1 (MQ=255)
                                                                                                            ggTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAgg                                     <  5:107300/149‑1 (MQ=255)
                                                                                                            ggTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAgg                                     >  1:166504/1‑149 (MQ=255)
                                                                                                             gTGC‑TGTGTGGGCAGGGCTGAAGAAAAACGCCTACACAAGTTGTCGGGGTTGGCACATATCAGTCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATTAATGCCTTGACTTCCGGGCTGGTCAggg                                    <  2:283781/149‑1 (MQ=255)
                                                                                                                c‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCt                                 >  5:75847/1‑149 (MQ=255)
                                                                                                                  tgtgCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCtt                                >  2:9213/1‑149 (MQ=255)
                                                                                                                    tgCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTgg                              <  1:170427/149‑1 (MQ=255)
                                                                                                                       gggCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                     >  2:41769/1‑107 (MQ=255)
                                                                                                                       gggCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                     <  1:41769/107‑1 (MQ=255)
                                                                                                                        ggCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAg                          >  6:290749/1‑149 (MQ=255)
                                                                                                                                  aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATTAATGCCTTGACTTCCGGTCTGGTCAGGGCCTTGGCCAGTTTCTGGa                  >  6:101269/1‑147 (MQ=255)
                                                                                                                                  aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                        <  7:295701/109‑1 (MQ=255)
                                                                                                                                  aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                        >  8:295701/1‑109 (MQ=255)
                                                                                                                                  aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                  <  5:101269/147‑1 (MQ=255)
                                                                                                                                  aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATg                >  5:104692/1‑149 (MQ=255)
                                                                                                                                       caaCGCCGACACCTTGTGTCGGCGTTGGCAGATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCa                           >  1:27339/2‑133 (MQ=255)
                                                                                                                                       caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCa                           <  2:27339/132‑1 (MQ=255)
                                                                                                                                       caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                  >  6:198815/2‑142 (MQ=255)
                                                                                                                                       caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                  <  5:198815/141‑1 (MQ=255)
                                                                                                                                        aaCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa          >  2:218925/1‑149 (MQ=255)
                                                                                                                                        aaCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa          <  4:11089/149‑1 (MQ=255)
                                                                                                                                               acacAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCt   >  3:216420/1‑149 (MQ=255)
                                                                                                                                                cacaAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCtt  >  2:13172/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTT  >  NC_002947/5890645‑5890936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑