Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,890,793	G→T	6.2%	intergenic (+81/+39)	PP_5164 → / ← plpB	penicillin amidase family protein/NLPA lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,890,793	0	G	T	6.2%	205.5 / 3.7	64	intergenic (+81/+39)	PP_5164/plpB	penicillin amidase family protein/NLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base T (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

AGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTTGT  >  NC_002947/5890645‑5890941                                                                                                                                                      |                                                                                                                                                     aGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                      >  2:310455/1‑149 (MQ=255)      cAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACCCCGACACAAGGTGTc                                                                                                                                                 >  7:189767/1‑149 (MQ=255)        gCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                               <  4:306602/149‑1 (MQ=255)         cGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                               <  1:159937/148‑1 (MQ=255)         cGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                               >  2:159937/1‑148 (MQ=255)              gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTgg                                                                                                                                         <  4:255952/149‑1 (MQ=255)              gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTgg                                                                                                                                         <  1:151559/149‑1 (MQ=255)              gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTgg                                                                                                                                         <  1:202230/149‑1 (MQ=255)                     ggggTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                                  <  3:82412/149‑1 (MQ=255)                      ggggGATTCCGGCGCAAACTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGTCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATc                                                                                                                                 <  8:265887/149‑1 (MQ=255)                             tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCa                                                                                                                                <  6:72447/143‑1 (MQ=255)                             tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCa                                                                                                                                >  5:72447/1‑143 (MQ=255)                             tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGtt                                                                                                                          >  6:107300/1‑149 (MQ=255)                               cGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGc                                                                                                                        >  5:306665/1‑149 (MQ=255)                                  cgcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAg                                                                                                                     >  2:170427/1‑149 (MQ=255)                                   gcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGc                                                                                                                    <  1:292629/149‑1 (MQ=255)                                    cAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                                  <  5:242301/134‑1 (MQ=255)                                    cAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                                  >  6:242301/1‑134 (MQ=255)                                          aCCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATc                                                                                                              <  2:20935/148‑1 (MQ=255)                                           ccTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAAt                                                                                                               >  2:221339/1‑146 (MQ=255)                                           ccTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCa                                                                                                             <  6:127329/148‑1 (MQ=255)                                                        aCCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTa                                                                                               >  6:111379/1‑149 (MQ=255)                                                         cccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTa                                                                                               <  6:306665/148‑1 (MQ=255)                                                                 gTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCtt                                                                                      >  6:236806/1‑149 (MQ=255)                                                                    agagGCTGCTTTGCGGTCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTg                                                                                     <  6:270692/147‑1 (MQ=255)                                                                    agagGCTGCTTTGCGGACGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTg                                                                                     >  5:270692/1‑147 (MQ=255)                                                                            ctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCAGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTg                                                                           >  1:61569/1‑149 (MQ=255)                                                                             tttGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                          >  4:154924/1‑149 (MQ=255)                                                                                   gCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatg                                                                     >  8:273966/1‑148 (MQ=255)                                                                                    ccGCTTCGCAGCAAAAGGCTTCTACGGTGCGTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatg                                                                     >  1:29493/1‑148 (MQ=255)                                                                                     cGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGaa                                                                                                                 >  4:22999/1‑102 (MQ=255)                                                                                     cGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGaa                                                                                                                 <  3:22999/102‑1 (MQ=255)                                                                                     cGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatgaat                                                                  >  3:11089/1‑149 (MQ=255)                                                                                          cGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGcc                                                               <  8:136557/147‑1 (MQ=255)                                                                                           gcagcaAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                             <  8:189767/148‑1 (MQ=255)                                                                                           gcagcaAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                             <  1:221339/148‑1 (MQ=255)                                                                                               caAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACtt                                                        <  2:29493/149‑1 (MQ=255)                                                                                                  aaGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCg                                                     >  3:284008/1‑149 (MQ=255)                                                                                                  aaGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAAAGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGAATTCCg                                                     >  4:198821/1‑149 (MQ=255)                                                                                                    ggCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                                   <  5:111379/149‑1 (MQ=255)                                                                                                    ggCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                                   <  7:273966/149‑1 (MQ=255)                                                                                                           aCGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGa                                                           >  3:267477/1‑134 (MQ=255)                                                                                                           aCGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGa                                                           <  4:267477/134‑1 (MQ=255)                                                                                                             ggTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAgg                                          <  5:107300/149‑1 (MQ=255)                                                                                                             ggTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAgg                                          >  1:166504/1‑149 (MQ=255)                                                                                                              gTGC‑TGTGTGGGCAGGGCTGAAGAAAAACGCCTACACAAGTTGTCGGGGTTGGCACATATCAGTCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATTAATGCCTTGACTTCCGGGCTGGTCAggg                                         <  2:283781/149‑1 (MQ=255)                                                                                                                 c‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCt                                      >  5:75847/1‑149 (MQ=255)                                                                                                                   tgtgCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCtt                                     >  2:9213/1‑149 (MQ=255)                                                                                                                     tgCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTgg                                   <  1:170427/149‑1 (MQ=255)                                                                                                                        gggCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                          >  2:41769/1‑107 (MQ=255)                                                                                                                        gggCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                          <  1:41769/107‑1 (MQ=255)                                                                                                                         ggCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAg                               >  6:290749/1‑149 (MQ=255)                                                                                                                                   aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATTAATGCCTTGACTTCCGGTCTGGTCAGGGCCTTGGCCAGTTTCTGGa                       >  6:101269/1‑147 (MQ=255)                                                                                                                                   aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                             <  7:295701/109‑1 (MQ=255)                                                                                                                                   aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                             >  8:295701/1‑109 (MQ=255)                                                                                                                                   aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                       <  5:101269/147‑1 (MQ=255)                                                                                                                                   aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATg                     >  5:104692/1‑149 (MQ=255)                                                                                                                                        caaCGCCGACACCTTGTGTCGGCGTTGGCAGATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCa                                >  1:27339/2‑133 (MQ=255)                                                                                                                                        caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCa                                <  2:27339/132‑1 (MQ=255)                                                                                                                                        caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                       >  6:198815/2‑142 (MQ=255)                                                                                                                                        caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                       <  5:198815/141‑1 (MQ=255)                                                                                                                                         aaCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa               <  4:11089/149‑1 (MQ=255)                                                                                                                                         aaCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa               >  2:218925/1‑149 (MQ=255)                                                                                                                                                acacAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCt        >  3:216420/1‑149 (MQ=255)                                                                                                                                                 cacaAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCtt       >  2:13172/1‑149 (MQ=255)                                                                                                                                                      ggTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTtgttgt  >  5:80446/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     AGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGC‑TGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTTGT  >  NC_002947/5890645‑5890941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑