Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,959,995	A→T	7.8%	noncoding (74/103 nt)	PP_mr63 ←	rnk_pseudo ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,959,995	0	A	T	7.8%	209.6 / 5.6	73	noncoding (74/103 nt)	PP_mr63	rnk_pseudo ncRNA
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base T (2/4);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

CGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCGT  >  NC_002947/5959849‑5960143                                                                                                                                                   |                                                                                                                                                     cGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAg                                                                                                                                                    <  2:167831/149‑1 (MQ=255)      tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCgg                                                                                                                                               >  1:144069/1‑149 (MQ=255)      tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCgg                                                                                                                                               >  6:36941/1‑149 (MQ=255)       ctcGTCGAGGCTGTCGAGCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                              <  2:115540/149‑1 (MQ=255)        tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCgg                                                                                                                                               <  4:196472/147‑1 (MQ=255)        tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTATTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                              >  5:276876/1‑148 (MQ=255)          gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                              <  6:276876/146‑1 (MQ=255)           tcgAGGCTGTCGATCCGACGCGCCAGACGCTGGACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCACTTTTCAATGTCCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGttt                                                                                                                                          <  2:93849/149‑1 (MQ=255)            cgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGtttt                                                                                                                                         <  7:273483/149‑2 (MQ=255)             gAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTg                                                                                                                                         <  2:231508/148‑1 (MQ=255)              aGGCGGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgc                                                                                                                                       <  6:332846/149‑4 (MQ=255)                 cTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAg                                                                                                                                    >  1:114846/1‑149 (MQ=255)                 cTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAg                                                                                                                                    <  1:139251/149‑1 (MQ=255)                    tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCt                                                                                                                                 >  8:128336/1‑149 (MQ=255)                      gATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                       >  1:297781/1‑141 (MQ=255)                      gATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTgag                                                                                                                                       <  2:297781/141‑1 (MQ=255)                          aGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                            <  2:299568/148‑1 (MQ=255)                                tCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAcc                                                                                                                      >  7:144697/1‑148 (MQ=255)                                 ccAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGa                                                                                                                    >  8:178224/1‑149 (MQ=255)                                     aCGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCa                                                                                                                >  7:192986/1‑149 (MQ=255)                                     aCGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCa                                                                                                                <  3:91904/149‑1 (MQ=255)                                      cGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAg                                                                                                               <  5:36941/149‑1 (MQ=255)                                          gTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAg                                                                                                                                    >  3:167707/1‑124 (MQ=255)                                          gTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAg                                                                                                                                    <  4:167707/124‑1 (MQ=255)                                           tACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAAcc                                                                                                           <  7:178224/148‑1 (MQ=255)                                            aCGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGAc                                                                                                                   >  8:14143/1‑139 (MQ=255)                                            aCGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGAc                                                                                                                   <  7:14143/139‑1 (MQ=255)                                             cGTGCAATAGGGTGAGGATGAGGGAAGGCTTTGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCTCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                        <  2:114846/149‑1 (MQ=255)                                             cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                        <  7:128336/149‑1 (MQ=255)                                             cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                        <  8:174826/149‑1 (MQ=255)                                                 cAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGacac                                                                                                     >  5:108802/1‑148 (MQ=255)                                                 cAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGacac                                                                                                     >  3:300208/1‑148 (MQ=255)                                                         tGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGc                                                                                            <  5:293253/149‑1 (MQ=255)                                                             gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                            <  8:121380/113‑1 (MQ=255)                                                             gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                            >  7:121380/1‑113 (MQ=255)                                                             gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCggg                                                                                        >  6:225942/1‑149 (MQ=255)                                                             gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCggg                                                                                        <  8:154567/149‑1 (MQ=255)                                                             gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCgg                                                                                         <  6:108802/148‑1 (MQ=255)                                                                gAGGGAAGGCTTGGTGCTCCTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCCATGTGCAACTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGCCGCAGACCCGGACGCTACCACAGCCGGGcc                                                                                      >  6:143197/1‑147 (MQ=255)                                                                  gggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAAt                                                                                   <  8:144697/149‑1 (MQ=255)                                                                   ggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATa                                                                                  >  2:296586/1‑149 (MQ=255)                                                                      aGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTc                                                                               <  5:225942/149‑1 (MQ=255)                                                                         cTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCCCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCaa                                                                             <  5:250163/148‑1 (MQ=255)                                                                                 tCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTgcg                                                                    >  8:66056/1‑149 (MQ=255)                                                                                 tCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTgcg                                                                    >  6:92646/1‑149 (MQ=255)                                                                                      aTCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCa                                                                <  1:304841/148‑1 (MQ=255)                                                                                        ccAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                            <  3:6478/86‑1 (MQ=255)                                                                                        ccAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                            >  4:6478/1‑86 (MQ=255)                                                                                                  tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGcc                                                   >  2:304096/1‑149 (MQ=255)                                                                                                  tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGcc                                                   >  7:66797/1‑149 (MQ=255)                                                                                                     tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACt                                                                                <  8:159901/117‑1 (MQ=255)                                                                                                     tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACt                                                                                >  7:159901/1‑117 (MQ=255)                                                                                                        gAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTcg                                             >  7:142114/1‑149 (MQ=255)                                                                                                         aaCAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTc                                                                               <  2:72612/114‑1 (MQ=255)                                                                                                         aaCAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTc                                                                               >  1:72612/1‑114 (MQ=255)                                                                                                              gCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                          >  6:70345/1‑146 (MQ=255)                                                                                                                  ttttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCTACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCt                                   <  8:142114/149‑1 (MQ=255)                                                                                                                     tCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                                 <  1:296586/148‑1 (MQ=255)                                                                                                                      cAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACCCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGc                               >  8:97526/1‑149 (MQ=255)                                                                                                                       aaTGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGACACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGcc                              >  1:88104/1‑149 (MQ=255)                                                                                                                            gCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                          <  2:88104/148‑1 (MQ=255)                                                                                                                             cAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGACTCGCgg                        >  8:116763/1‑149 (MQ=255)                                                                                                                               aaTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAATTACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                        >  4:94393/1‑147 (MQ=255)                                                                                                                                     gCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTg                >  2:25924/1‑149 (MQ=255)                                                                                                                                      cAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGc               >  7:182384/1‑149 (MQ=255)                                                                                                                                       caaaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGcc              >  8:29232/2‑149 (MQ=255)                                                                                                                                       aaaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGcc              <  2:144069/149‑1 (MQ=255)                                                                                                                                       aaaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATATGcc              >  7:76915/1‑149 (MQ=255)                                                                                                                                          aCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgc           >  8:142360/1‑149 (MQ=255)                                                                                                                                            cccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgcgc         <  8:66797/149‑1 (MQ=255)                                                                                                                                             ccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCTCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGcc        >  1:165042/1‑149 (MQ=255)                                                                                                                                                cccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                                 >  6:253507/1‑121 (MQ=255)                                                                                                                                                cccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                                 <  5:253507/121‑1 (MQ=255)                                                                                                                                                   aaGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCGt  >  1:324006/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTCGT  >  NC_002947/5959849‑5960143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑