Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,966,333 A→T 7.6% intergenic (‑402/+28) PP_5232 ← / ← amtB hypothetical protein/ammonium transporter AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,966,3330AT 7.6% 184.8 / 3.2 63intergenic (‑402/+28)PP_5232/amtBhypothetical protein/ammonium transporter AmtB
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base T (2/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGACGA  >  NC_002947/5966188‑5966479
                                                                                                                                                 |                                                                                                                                                  
gcgcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGcc                                                                                                                                                 >  2:97998/1‑149 (MQ=255)
   cgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTATACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCggg                                                                                                                                              <  2:163048/149‑1 (MQ=255)
      aTTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCat                                                                                                                                           >  2:193381/1‑148 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtttt                                                                                                                                      <  8:186099/148‑1 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtttt                                                                                                                                      <  5:226876/148‑1 (MQ=255)
              gcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCATttttttgc                                                                                                                                   <  6:322744/149‑3 (MQ=255)
                gcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGcca                                                                                                                                 <  1:193381/149‑2 (MQ=38)
                    gTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTc                                                                                                                             <  8:39705/149‑1 (MQ=255)
                         aaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGt                                                                                                                        >  6:22764/1‑149 (MQ=255)
                         aaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGt                                                                                                                        >  6:113418/1‑149 (MQ=255)
                            agcaaaccaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                                      >  6:262522/1‑148 (MQ=255)
                              caaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAg                                                                                                                   <  1:212004/149‑1 (MQ=255)
                                   caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCCGGAAATGCTGAAAAACTGGCAAAAAAATGCCCTGCAAGCCGGGCAAGTTTTTAAAGCGTAGAAGTTACAGGTTg                                                                                                               >  4:102167/1‑148 (MQ=255)
                                    aCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGt                                                                                                              <  5:59111/148‑1 (MQ=255)
                                     ccATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAg                                                                                                            >  1:136630/1‑149 (MQ=255)
                                          aaCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcgcg                                                                                                       >  3:46854/1‑149 (MQ=255)
                                             gCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTc                                                                                                    >  6:124086/1‑149 (MQ=255)
                                                cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                                 <  2:136630/149‑1 (MQ=255)
                                                cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                                 <  5:113418/149‑1 (MQ=255)
                                                   cGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtg                                                                                                <  1:97998/147‑1 (MQ=255)
                                                        tGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAg                                                                                         >  1:278245/1‑149 (MQ=255)
                                                         gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCATGCCGTGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAg                                                                                         <  3:102167/148‑1 (MQ=255)
                                                          tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGc                                                                                        <  5:22764/148‑1 (MQ=255)
                                                            aGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                                     <  5:262522/149‑1 (MQ=255)
                                                            aGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                                     >  5:23454/1‑149 (MQ=255)
                                                               tttttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGt                                                                                   <  2:278245/148‑1 (MQ=255)
                                                                     ccTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGcc                                                                            >  1:317053/1‑149 (MQ=255)
                                                                         cGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgac                                                                         <  5:124086/148‑1 (MQ=255)
                                                                            cACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTACTGAGCCAGGTCGAGGCcgaccgac                                                                     >  5:280288/1‑149 (MQ=255)
                                                                             aCGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCcgaccgac                                                                     <  8:119524/148‑1 (MQ=255)
                                                                               gAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGTAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGActct                                                                  >  4:8421/1‑149 (MQ=255)
                                                                               gAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgacc                                                                        <  6:207925/143‑1 (MQ=255)
                                                                               gAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgacc                                                                        >  5:207925/1‑143 (MQ=255)
                                                                                    tgtCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttcttc                                                             >  8:140814/1‑149 (MQ=255)
                                                                                      tCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGTAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTcttcttctt                                                           <  3:8421/149‑1 (MQ=255)
                                                                                      tCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTcttcttctt                                                           >  5:145822/1‑149 (MQ=255)
                                                                                        gAATAACCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                                          <  6:9250/148‑1 (MQ=255)
                                                                                        gAATAAACCGTTTGCCGTGCGGTAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCg                                                         <  6:324209/149‑1 (MQ=255)
                                                                                         aaTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttct                                                               >  1:10417/1‑142 (MQ=255)
                                                                                         aaTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttct                                                               <  2:10417/142‑1 (MQ=255)
                                                                                         aaTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                                        >  5:262208/1‑149 (MQ=255)
                                                                                           tAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGtt                                                       >  8:117089/1‑148 (MQ=255)
                                                                                             aaCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAc                                                    <  2:317053/149‑1 (MQ=255)
                                                                                              aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAcc                                                   >  3:137025/1‑149 (MQ=255)
                                                                                              aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAcc                                                   >  5:218943/1‑149 (MQ=255)
                                                                                                      ccGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGc                                            <  7:140814/148‑1 (MQ=255)
                                                                                                            gggAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATcac                                     >  8:18695/1‑149 (MQ=255)
                                                                                                                aaagcagAAAAACTGGCAAAAAAATCCCCGGCAAGCCCGGCAACTAATTACCGCGTCGCAGTTACAGGTTGTACCCGCCCTCGTGCTGCTGCGCCAGGTCGAGGCCGCCCGCCTGTTCTAGTTCGTTCACCCGCAGGCGCATcccca                                   >  8:257051/7‑147 (MQ=255)
                                                                                                                       aaaaaCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                                        >  2:299977/1‑119 (MQ=255)
                                                                                                                       aaaaaCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                                        <  1:299977/119‑1 (MQ=255)
                                                                                                                           aCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCAc                         >  6:229662/1‑146 (MQ=255)
                                                                                                                           aCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCAc                         <  5:229662/146‑1 (MQ=255)
                                                                                                                             tGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCtt                      >  1:70612/1‑147 (MQ=255)
                                                                                                                               gCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTgag                   <  4:137025/148‑1 (MQ=255)
                                                                                                                                 aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGaa                 >  8:190112/1‑148 (MQ=255)
                                                                                                                                      aaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGt           <  7:190112/149‑1 (MQ=255)
                                                                                                                                       ttGCCCGGCTTGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACg            >  4:207999/2‑147 (MQ=255)
                                                                                                                                          cccGGCAAGCCGGGCAAGTTTTTAGCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGCCCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGAACCTTGAGAATCACGTAGGt       >  7:44605/1‑149 (MQ=255)
                                                                                                                                           ccGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAgg                                             >  7:267141/1‑110 (MQ=255)
                                                                                                                                           ccGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAgg                                             <  8:267141/110‑1 (MQ=255)
                                                                                                                                            cGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttcttc                                                             >  4:91589/1‑93 (MQ=255)
                                                                                                                                            cGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttcttc                                                             <  3:91589/93‑1 (MQ=255)
                                                                                                                                               cAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGACGa  <  6:145822/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGACGA  >  NC_002947/5966188‑5966479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑