Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,980,189	G→A	7.3%	W278* (TGG→TGA)	PP_5242 →	GAF domain‑containing sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,980,189	0	G	A	7.3%	200.4 / 3.4	68	W278* (TGG→TGA)	PP_5242	GAF domain‑containing sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base A (2/3);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAA  >  NC_002947/5980044‑5980333                                                                                                                                                  |                                                                                                                                                  aGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCCCGGCCGCGGGCAGGGGgcc                                                                                                                                                >  1:202783/1‑149 (MQ=255)   ccGCGGGCGGCCAGCGCAGCGGACAGCAAGGGGCCGCACTCCCGCAGCCTGGGCCTGGCAACGGCCGTGGCCAACGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCCCACTGCCGCTGGCAGTGAgccaa                                                                                                                                              <  4:189903/149‑3 (MQ=255)   ccGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGgccgc                                                                                                                                              <  8:123435/149‑1 (MQ=255)   ccGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGgccgc                                                                                                                                              <  8:127741/149‑1 (MQ=255)    cgcgGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGgccgcc                                                                                                                                             >  6:9191/1‑149 (MQ=255)       gggcggCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCca                                                                                                                                           >  1:116717/1‑148 (MQ=255)        ggcggcCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCcac                                                                                                                                          >  2:305928/1‑148 (MQ=255)           ggcCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACaaaa                                                                                                                                      >  6:165636/1‑149 (MQ=255)           ggcCAGCGAAGCGGACAGCAAGGCGCCGCACTCCCTCAGCCTGGGCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACaaaa                                                                                                                                      <  5:233661/149‑1 (MQ=255)            gcCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAAc                                                                                                                                     <  5:9191/149‑1 (MQ=255)             ccAGCGGAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAAc                                                                                                                                     >  8:290894/1‑148 (MQ=255)                     gCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAcc                                                                                                                            >  1:216500/1‑149 (MQ=255)                           aGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCAc                                                                                                                      <  2:205132/149‑1 (MQ=255)                             cAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCAccc                                                                                                                    <  8:3817/149‑1 (MQ=255)                                ggTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAg                                                                                                                 <  6:233535/149‑1 (MQ=255)                                 gTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAgg                                                                                                                <  2:216500/149‑1 (MQ=255)                                   gCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATg                                                                                                                                 >  3:142786/1‑130 (MQ=255)                                   gCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATg                                                                                                                                 <  4:142786/130‑1 (MQ=255)                                          tCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCCCACTGCCGCTGGCAGTGAgccaa                                                                                                                                              >  3:145138/1‑108 (MQ=255)                                          tCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCCCACTGCCGCTGGCAGTGAgccaa                                                                                                                                              <  4:145138/110‑3 (MQ=255)                                            ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGAcc                                                                                                     >  6:40832/1‑149 (MQ=255)                                            ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGAcc                                                                                                     <  2:305305/149‑1 (MQ=255)                                            ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGAcc                                                                                                     >  5:259351/1‑149 (MQ=255)                                            ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGAcc                                                                                                     >  4:301197/1‑149 (MQ=255)                                             cTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGAccc                                                                                                    <  2:116717/149‑1 (MQ=255)                                                aGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCgg                                                                                                  <  7:290894/148‑1 (MQ=255)                                                aGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCgg                                                                                                  <  5:165636/148‑1 (MQ=255)                                                      gTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATg                                                                                                                                 >  8:40686/1‑111 (MQ=255)                                                      gTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATg                                                                                                                                 <  7:40686/111‑1 (MQ=255)                                                        ccTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGCGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCCGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCg                                                                                         >  5:261235/1‑149 (MQ=255)                                                         cTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCgg                                                                                        >  2:134737/1‑149 (MQ=255)                                                            gCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCa                                                                                     >  2:110368/1‑149 (MQ=255)                                                             cAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCAc                                                                                    >  6:48825/1‑149 (MQ=255)                                                             cAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCAc                                                                                    <  1:134737/149‑1 (MQ=255)                                                              aaaGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCAcg                                                                                   >  4:222883/1‑149 (MQ=255)                                                                 ggCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCAcgcgc                                                                                <  5:48825/149‑1 (MQ=255)                                                                        gCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCCCCCCAGGCTGCGCCGCCCCGGTAGCGCCCGGCCACGCGCACCT‑Gtt                                                                        >  4:82059/1‑149 (MQ=255)                                                                          cAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGTCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTg                                                                      >  4:224001/1‑149 (MQ=255)                                                                          cAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTg                                                                      >  5:96763/1‑149 (MQ=255)                                                                          cAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTg                                                                      >  3:306036/1‑149 (MQ=255)                                                                           aaaGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTgg                                                                     <  3:222883/149‑1 (MQ=255)                                                                                 ccGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCgcgg                                                               <  1:305928/149‑1 (MQ=255)                                                                                     aCGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGcc                                                           >  5:152345/1‑149 (MQ=255)                                                                                         ccGCTACCAGA‑‑GCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCa                                                      >  1:45370/1‑148 (MQ=255)                                                                                          cGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGc                                                       >  1:98326/1‑148 (MQ=255)                                                                                               ccAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCaaaa                                                   <  5:290673/147‑1 (MQ=255)                                                                                                        gcgcgcCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCAccc                                        >  5:90293/1‑149 (MQ=255)                                                                                                            gcCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCctgct                                    >  7:2384/1‑149 (MQ=255)                                                                                                                ttGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTgctgct                                 >  5:120812/1‑148 (MQ=255)                                                                                                                  gCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCctgct                                    <  4:299533/143‑1 (MQ=255)                                                                                                                  gCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCctgct                                    >  3:299533/1‑143 (MQ=255)                                                                                                                   cAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAg                             <  4:306036/149‑1 (MQ=255)                                                                                                                    aGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAgg                            >  1:292994/1‑149 (MQ=255)                                                                                                                        gACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCtt                        <  6:259351/149‑1 (MQ=255)                                                                                                                        gACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCt                         <  8:2384/148‑1 (MQ=255)                                                                                                                          ggcggCCCACTGCCGCTGGCAGTGAGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTAt                                             <  6:298890/123‑1 (MQ=255)                                                                                                                          ggcggCCCACTGCCGCTGGCAGTGAGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTAt                                             >  5:298890/4‑126 (MQ=255)                                                                                                                             ggCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTCGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAg                             <  1:71762/140‑1 (MQ=255)                                                                                                                             ggCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCgcgg                                                               <  6:149347/105‑1 (MQ=255)                                                                                                                             ggCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCgcgg                                                               >  5:149347/1‑105 (MQ=255)                                                                                                                             ggCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAg                             >  2:71762/1‑139 (MQ=255)                                                                                                                              gCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCt                         <  2:273106/142‑1 (MQ=255)                                                                                                                                  aCTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgcg                <  6:120812/147‑1 (MQ=255)                                                                                                                                  aCTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgcg                <  3:301197/147‑1 (MQ=255)                                                                                                                                  aCTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgcg                <  5:40832/147‑1 (MQ=255)                                                                                                                                           ggCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACca     <  2:292994/149‑1 (MQ=255)                                                                                                                                            gCAGTGGGTCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACcac    <  3:224001/149‑1 (MQ=255)                                                                                                                                              aGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACaa  <  2:45370/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  AGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCT‑GTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAA  >  NC_002947/5980044‑5980333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑