Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,013,885	T→C	100%	intergenic (+90/+20)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,885	0	T	C	100.0%	123.1 / NA	37	intergenic (+90/+20)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAA  >  NC_002947/6013740‑6014030                                                                                                                                                    |                                                                                                                                                  aaTCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAACCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGc                                                                                                                                                 <  6:93595/148‑1 (MQ=255)     gCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTTGCGGGCACGCCCGCTCCCACAGGTACGGCCCAGCGGTCGAGCCCTGTGCTGGACCGGTGGG‑‑GGCGGGCGTGCCCGCGaag                                                                                                                                             >  3:309684/1‑147 (MQ=21)                  aaGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCtt                                                                                                                                <  8:303352/148‑1 (MQ=255)                   aGTCGCTGGAAGGGGGTGGGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCtttt                                                                                                                              <  1:231274/149‑1 (MQ=255)                        cTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                                                         <  8:253331/149‑1 (MQ=255)                                 ggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgcc                                                                                                                <  5:129602/149‑1 (MQ=255)                                  gtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCGCGCCCCCGCCCACACGTGCGGCGCACCGGTCGAACCCGGTGGGGGGCCGGTGGG‑‑GGCGCGCGGGGCCCCGAAAGGCCGCCACCCGTTTTTATGGTTggccccc                                                                                                               >  2:103622/1‑143 (MQ=255)                                            cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCAACCACAGGTGCAACACACGGCTCGAACCCAGTGCCGTACCATTGGG‑‑AGCGGGCGTGCCCGCGAAATGCCGCAACCCATTTATCTGGTTGCCGACAGACCCGCca                                                                                                      >  2:251035/1‑148 (MQ=255)                                                      tAAACGCGACACCTGCTGCGCGGGCACGCCCGCTCCCACAGGTTCAGCACAGGTCTCTAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAgcgc                                                                                           <  6:178544/149‑1 (MQ=255)                                                      tAAACGAGACCCCTGCTTCGCGGTCACGCCCGCTCCCTCAGGTGCAGCACACGTCTCGAACGCTGTGCCTTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAgcgc                                                                                           <  7:115453/149‑1 (MQ=38)                                                                 ccTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                                                                                <  1:239791/149‑1 (MQ=255)                                                                        cgcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                                                                           <  6:278847/147‑1 (MQ=255)                                                                             gCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGccc                                                                    <  1:214130/149‑1 (MQ=255)                                                                                     gccccccCAGGTGCACCACACGGCTCCTACGCTGTGCCGTACCTGGGGGTTCCCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                                              <  1:103622/146‑1 (MQ=255)                                                                                          cacaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                                        >  3:59946/1‑148 (MQ=255)                                                                                          cacaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                                        >  1:309492/1‑148 (MQ=255)                                                                                            caGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCaa                                                     <  2:309492/149‑1 (MQ=255)                                                                                                   agcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                              >  3:57718/1‑149 (MQ=255)                                                                                                      accggTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGc                                           <  2:96166/146‑1 (MQ=255)                                                                                                        aGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                                         >  3:79236/1‑101 (MQ=255)                                                                                                        aGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                                         <  4:79236/101‑1 (MQ=255)                                                                                                           tctcGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGc                                                                                                        <  1:241002/83‑1 (MQ=255)                                                                                                           tctcGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCACCGc                                                                                                        >  2:241002/1‑83 (MQ=255)                                                                                                               gAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                                  >  4:96008/1‑149 (MQ=255)                                                                                                               gAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                                   <  4:59946/148‑1 (MQ=255)                                                                                                                 aCGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTCCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt                                >  8:78442/1‑149 (MQ=255)                                                                                                                     tgtgCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTc                             >  8:203865/1‑148 (MQ=255)                                                                                                                       tgCCGTACCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATa                          >  4:58735/1‑149 (MQ=255)                                                                                                                           gcaCCTGTGGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAcccc                      >  5:188890/3‑149 (MQ=255)                                                                                                                                 gtgGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGc                >  1:64734/1‑149 (MQ=255)                                                                                                                                  tgGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTcc                   <  2:130002/145‑1 (MQ=255)                                                                                                                                  tgGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTcc                   >  1:130002/1‑145 (MQ=255)                                                                                                                                  tgGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCgg                 <  5:229150/147‑1 (MQ=255)                                                                                                                                  tgGG‑‑AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCgg                 >  6:229150/1‑147 (MQ=255)                                                                                                                                         gCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGt          <  1:12484/149‑1 (MQ=255)                                                                                                                                              cGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATc     >  2:11344/1‑149 (MQ=255)                                                                                                                                                 gCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCaa  >  4:215924/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGG‑‑AGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAA  >  NC_002947/6013740‑6014030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑